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FITC Rat IgG1, kappa [RTK2071] - isotype control (ab18404)

Q&A (2)

Conjugates logo Related conjugates and formulations

Biotin Unconjugated

製品の概要

  • 製品名

    FITC Rat IgG1, kappa [RTK2071] - isotype control
    Rat アイソタイプ・コントロール 製品一覧

  • 標識

    FITC. Ex: 493nm, Em: 528nm

  • 特記事項（標識）

    The antibody was conjugated with FITC under optimal conditions. The solution is free of unconjugated FITC.

  • 特異性

    The RTK2071 immunoglobulin reacts with KLH. The RTK2071 immunoglobulin was chosen as an isotype control after screening on a variety of resting, activated, live, and fixed mouse and human tissues.

  • アプリケーション

    適用あり: Flow Cytmore details

  • 免疫原

    Trinitrophenol and KLH.

  • 特記事項

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C.

  • バッファー

    pH: 7.20
    Preservative: 0.09% Sodium azide
    Constituent: PBS
  • Concentration information loading...

  • 精製度

    Immunogen affinity purified

  • ポリ/モノ

    モノクローナル

  • クローン名

    RTK2071

  • アイソタイプ

    IgG1

  • 軽鎖の種類

    kappa

  • 研究分野

  • 別名

    • Rat Isotype Control

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab18404の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
Flow Cyt
Use at an assay dependent dilution.
特記事項
Flow Cyt
Use at an assay dependent dilution.

参考文献 (0)

ab18404 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab18404 は論文での使用が確認できていません。

レビューと Q&A

Q&A
質問を送る

1-2 of 2 Q&A

Question

Thank you for your help.
I have two additional questions:
- what do you mean as " - no primary - only secondary control" ?
- can you provide a protocol to use the isotype control you suggest?
Thank you for your collaboration

Read More
Answer

Thank you for your response.

"No primary control" means that you perform the staining protocol as normal (fixation, blocking, secondary antibody, detection) - except you need to omit the incubation step with the primary antibody. Instead of applying the primary antibody, you only incubate the samples with the dilution buffer you would normally use for diluting the primary antibody.

Isotype control meansyou perform the staining protocol as normal butt the samples will be treated with the isotype control instead of the primary antibody. The isotype of this primary antibody is IgG1, so the isotype control is rat IgG1 .

I would advise you to visit this site to get some further information about positive control and isotype control:

https://www.abcam.com/index.html?pageconfig=technicalfaqs

I hope this helps and if I can assist further, please do not hesitate to contact me.

Read More

Question

Inquiry: Dear custumer service, we recently used your HCN4 antibody (ab32675) to perform an immunofluorescence assay on human atrial and ventricular myocytes. Staining was succesful and this data were submitted for publication. One of the reviewer asked to have a negative control, e.g. a cell stained with the HCN4 antibody pre-absorbed with the specific peptide. How can we meet this request? do you have a specific peptide and a suitable protocol to perform this assay? thank you for your assistance. Best

Read More
Answer

Thank you for your enquiry and your interest in our products.

The immunogen used to raise this antibody was a synthetic peptide: SHGSLLLPPA SSPPPPQVPQ RRGTPPLTPG RLTQDLKL, corresponding to amino acids 1048-1085 of Human HCN4:SHGSLLLPPASSPPPPQVPQRRGTPPLTPGRLTQDLKL. Unfortunately, the peptide is not commercially available.

Even though the immunogen peptide is not available, you could apply several controls to check the specificity of this antibody:

- isotype control,

- negative control,

- no primary - only secondary control

The isotype of this antibody is IgG1 so you can apply an isotype control using rat IgG1 (ab46745, ab128477, ab18403, ab18404, ab18407).

HCN4 is highly expressed in thalamus, testis and in heart, both in ventricle and atrium. It is detected at much lower levels in amygdala, substantia nigra, cerebellum and hippocampus. Generally, negative controls are cells/tissue which do not express the target protein or at very low level. You may find some useful information about expression level of HCN4 at this site: http://www.proteinatlas.org/ENSG00000138622

I hope this helps and if I can assist further, please do not hesitate to contact me.

Read More

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