FITC Anti-Myeloperoxidase 抗体 [2C7] (ab11729)
![Flow Cytometry (Intracellular) - FITC Anti-Myeloperoxidase antibody [2C7] (ab11729)](https://www.abcam.co.jp/ps/products/11/ab11729/Images/ab11729-273583-anti-myeloperoxidase-antibody-2c7-fitc-flow-cytometry.gif "Flow Cytometry (Intracellular) - FITC Anti-Myeloperoxidase antibody [2C7] (ab11729)")
Key features and details
- FITC Mouse monoclonal [2C7] to Myeloperoxidase
- Suitable for: Flow Cyt (Intra)
- Reacts with: Human
- Conjugation: FITC. Ex: 493nm, Em: 528nm
- Isotype: IgG1
Related conjugates and formulations
製品の概要
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製品名
FITC Anti-Myeloperoxidase antibody [2C7]
Myeloperoxidase 一次抗体 製品一覧 -
製品の詳細
FITC Mouse monoclonal [2C7] to Myeloperoxidase -
由来種
Mouse -
標識
FITC. Ex: 493nm, Em: 528nm -
アプリケーション
適用あり: Flow Cyt (Intra)more details -
種交差性
交差種: Human -
免疫原
Recombinant full length protein corresponding to Human Myeloperoxidase.
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ポジティブ･コントロール
- human peripheral blood granulocytes: Flow Cyt (Intra).
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特記事項
This product should be stored undiluted. Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. -
バッファー
pH: 7.40
Preservative: 0.09% Sodium azide
Constituents: PBS, 1% BSA -
Concentration information loading... -
精製度
Protein G purified -
特記事項（精製）
Purified IgG prepared by affinity chromatography. -
ポリ/モノ
モノクローナル -
クローン名
2C7 -
ミエローマ
x63-Ag8.653 -
アイソタイプ
IgG1 -
研究分野
関連製品
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Alternative Versions
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Assay kits
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab11729の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| Flow Cyt (Intra) |
1/1 - 1/10.
Use 10µl of the suggested working dilution to label 106 cells in 100µl. This antibody may be used for the flow cytometric detection of myeloid cells following membrane permeablisation. ab91356 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
| 特記事項 |
|---|
|
Flow Cyt (Intra)
1/1 - 1/10. Use 10µl of the suggested working dilution to label 106 cells in 100µl. This antibody may be used for the flow cytometric detection of myeloid cells following membrane permeablisation. ab91356 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
ターゲット情報
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機能
Part of the host defense system of polymorphonuclear leukocytes. It is responsible for microbicidal activity against a wide range of organisms. In the stimulated PMN, MPO catalyzes the production of hypohalous acids, primarily hypochlorous acid in physiologic situations, and other toxic intermediates that greatly enhance PMN microbicidal activity. -
関連疾患
Defects in MPO are the cause of myeloperoxidase deficiency (MPD) [MIM:254600]. MPD is an autosomal recessive defect that results in disseminated candidiasis. -
配列類似性
Belongs to the peroxidase family. XPO subfamily. -
細胞内局在
Lysosome. - Information by UniProt
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参照データベース
- Entrez Gene: 4353 Human
- Omim: 606989 Human
- SwissProt: P05164 Human
- Unigene: 458272 Human
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別名
- 84 kDa myeloperoxidase antibody
- 89 kDa myeloperoxidase antibody
- EC 1.11.1.7 antibody
see all
画像
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Flow Cytometry (Intracellular) - FITC Anti-Myeloperoxidase antibody [2C7] (ab11729)
Staining of human peripheral blood granulocytes with ab11729 following permeabilisation with Leucoperm
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (12)
ab11729 は 12 報の論文で使用されています。
- Schneck E et al. Flow Cytometry-Based Quantification of Neutrophil Extracellular Traps Shows an Association with Hypercoagulation in Septic Shock and Hypocoagulation in Postsurgical Systemic Inflammation-A Proof-of-Concept Study. J Clin Med 9:N/A (2020). PubMed: 31936385
- Schneck E et al. Blood Levels of Free-Circulating Mitochondrial DNA in Septic Shock and Postsurgical Systemic Inflammation and Its Influence on Coagulation: A Secondary Analysis of a Prospective Observational Study. J Clin Med 9:N/A (2020). PubMed: 32629885
- Manda-Handzlik A et al. Nitric oxide and peroxynitrite trigger and enhance release of neutrophil extracellular traps. Cell Mol Life Sci 77:3059-3075 (2020). PubMed: 31650185
- Mroczek A et al. Overexpression of ATG5 Gene Makes Granulocyte-Like HL-60 Susceptible to Release Reactive Oxygen Species. Int J Mol Sci 21:N/A (2020). PubMed: 32707918
- Kuzmicka W et al. Zinc Supplementation Modulates NETs Release and Neutrophils' Degranulation. Nutrients 13:N/A (2020). PubMed: 33375275
