FITC Anti-Myc tag 抗体 (ab1393)

Reviews (1)Q&A (1)References (3)

Key features and details

  • FITC Rabbit polyclonal to Myc tag
  • Suitable for: WB, ELISA
  • Conjugation: FITC. Ex: 493nm, Em: 528nm
  • Isotype: IgG

製品の概要

  • 製品名

    FITC Anti-Myc tag antibody
    Myc tag 一次抗体 製品一覧

  • 製品の詳細

    FITC Rabbit polyclonal to Myc tag

  • 由来種

    Rabbit

  • 標識

    FITC. Ex: 493nm, Em: 528nm

  • アプリケーション

    適用あり: WB, ELISAmore details

  • 免疫原

    EQKLISEEDL (C-MYC) conjugated with KLH.

  • 特記事項

    Molar F/P ratio is 4.6:1

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C.

  • バッファー

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 1.19% HEPES, 0.58% Sodium chloride, 0.2% BSA
  • Concentration information loading...

  • 精製度

    Immunogen affinity purified

  • 特記事項（精製）

    Antibodies were immunoaffinity purified using the peptide conjugated to a solid-phase support and conjugated to fluorescein isothiocyanate.

  • ポリ/モノ

    ポリクローナル

  • アイソタイプ

    IgG

  • 研究分野

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab1393 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000 - 1/5000. Can be blocked with Human c-Myc peptide (ab13837).
ELISA 1/1000.

ターゲット情報

  • 関連性

    Epitope tags are short peptide sequences that are easily recognized by tag-specific antibodies. Due to their small size, epitope tags do not affect the tagged protein’s biochemical properties. Most often sequences encoding the epitope tag are included with target DNA at the time of cloning to produce fusion proteins containing the epitope tag sequence. This allows anti-epitope tag antibodies to serve as universal detection reagents for any tag containing protein produced by recombinant means. This means that anti-epitope tag antibodies are a useful alternative to generating specific antibodies to identify, immunoprecipitate or immunoaffinity purify a recombinant protein. The anti-epitope tag antibody is usually functional in a variety of antibody-dependent experimental procedures. Expression vectors producing epitope tag fusion proteins are available for a variety of host expression systems including bacteria, yeast, insect and mammalian cells.

  • 細胞内局在

    Nuclear

  • 別名

    • c-myc tag antibody
    • Myc Epitope Tag antibody

参考文献 (3)

ab1393 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab1393 は 3 報の論文で使用されています。

  • Najjar F  et al. Sex differences in depression-like behavior and neuroinflammation in rats post-MI: role of estrogens. Am J Physiol Heart Circ Physiol 315:H1159-H1173 (2018). PubMed: 30052050
  • Jain M  et al. Linear assembly of a human centromere on the Y chromosome. Nat Biotechnol 36:321-323 (2018). PubMed: 29553574
  • Xiao C  et al. Targeting HSP70 to motoneurons protects locomotor activity from hyperthermia in Drosophila. Dev Neurobiol 67:438-55 (2007). PubMed: 17443800

レビューと Q&A

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1-2 of 2 Abreviews or Q&A

Flow Cytometry abreview for Anti-Myc tag antibody (FITC)

 Excellent
Abreviews
abreview image
Application
Flow Cytometry
Sample
Saccharomyces cerevisiae Cell (Yeast cells)
Permeabilization
No
Gating Strategy
Cells that were Myc-FITC positive
Specification
Yeast cells
Preparation
Cell harvesting/tissue preparation method: Harvested them in standard PBS with 0.1% BSA
Sample buffer: PBS with 0.1% BSA
Fixation
No fixiation
Read More

Dr. Kaushik Thakkar

Verified customer

投稿 Jul 03 2019

Question

To the attention of: Abcam, Technical support
Concerns : ab1393, rabbit polyclonal to MYC tag, FITC
Dear Mrs, Mr,
I searched the Abcam website about this product. There is one scientific reference, but the application is very specific and does not help us.
Do you have more information to use this product for immunofluorescence on cultured cells ? (blocking solution, incubation time,…)
Sincerely yours,

Read More
Answer

Thank you very much for your interest in ab1393.

Unfortunately we do not have more information about the use of the anti-Myc tag antibody (FITC) ab1393 in ICC/IF than the one available in the publication (PubMed: 17443800) and we do not currently have an image.

However by participating in our AbTrial program you can now use our products in an untested application or species without financial risk.

Simply follow these easy steps below to apply for our AbTrial Program:
1. Reply to this email, letting us know you are interested in testing this product.
2. Our scientists will email you an inactive personal discount code for the value of the product.
3. Purchase and test the product at the regular price.
4. Submit an image of your results, including your discount code in the additional notes section of your Abreview.
5. Once the Abreview is submitted, the discount code will become active.
6. Apply your discount code on your next order to receive that value off.

Please let me know if you have any questions about this offer and I would be happy to help you further.

The Terms and Conditions of this offer can be found at: https://www.abcam.com/abtrial.

About the ICC/IF protocol, we recommend the following procedure: https://www.abcam.com/index.html?pageconfig=resource&rid=11417.

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