FITC Mouse IgG1, monoclonal [HybIgG1]  - Isotype Control (ab1264)

Q&A (3)References (1)

製品の概要

  • 製品名

    FITC Mouse IgG1, monoclonal [HybIgG1]  - Isotype Control
    Mouse アイソタイプ・コントロール 製品一覧

  • 製品の詳細

    FITC Mouse IgG1, monoclonal [HybIgG1] - Isotype Control

  • 標識

    FITC. Ex: 493nm, Em: 528nm

  • アプリケーション

    適用あり: Flow Cytmore details

  • 特記事項

    This antibody is a direct immunofluorescence reagent used as an isotype (negative) control for flow cytometric immunophenotyping of erythrocyte-lysed whole blood with monoclonal antibodies.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C.

  • バッファー

    Preservative: 0.1% Sodium azide
    Constituent: 0.5% BSA
  • Concentration information loading...

  • アイソタイプ・コントロール 備考

    This antibody is a direct immunofluorescence reagent used as an isotype (negative) control for flow cytometric immunophenotyping of erythrocyte-lysed whole blood with monoclonal antibodies.

  • ポリ/モノ

    モノクローナル

  • クローン名

    HybIgG1

  • ミエローマ

    unknown

  • アイソタイプ

    IgG1

  • 軽鎖の種類

    unknown

  • 研究分野

  • 別名

    • Mouse Isotype Control

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab1264の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
Flow Cyt
Use at an assay dependent concentration. This antibody is used to determine the unstained lymphocyte population and to determine any non-antigen-specific antibody binding on mononuclear cells separated by density gradient or on lysed whole blood. IgG1 (FITC) immunofluorescence analysis can be performed on a flow cytometer equipped with an excitation source of 488nm and fitted with logarithmic amplifiers. 10µl ofIgG1 (FITC) is sufficient for labelling of 1x106 cells. Molecular F/P ratio: 3.0-6.0
特記事項
Flow Cyt
Use at an assay dependent concentration. This antibody is used to determine the unstained lymphocyte population and to determine any non-antigen-specific antibody binding on mononuclear cells separated by density gradient or on lysed whole blood. IgG1 (FITC) immunofluorescence analysis can be performed on a flow cytometer equipped with an excitation source of 488nm and fitted with logarithmic amplifiers. 10µl ofIgG1 (FITC) is sufficient for labelling of 1x106 cells. Molecular F/P ratio: 3.0-6.0

参考文献 (1)

ab1264 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab1264 は 1 報の論文で使用されています。

  • Flores S  et al. Characterization of the Glucocorticoid Receptor in Children Undergoing Cardiac Surgery. Pediatr Crit Care Med 19:705-712 (2018). PubMed: 29677033

レビューと Q&A

Q&A
質問を送る

1-3 of 3 Q&A

Question

Testing discount codes for ab105439 & ab97715 to test in flowcytometry.

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Answer

DISCOUNT CODE: *********- For ab105439
DISCOUNT CODE: *******- For ab97715
Expiration date: *************

I am very pleased to hear you would like to accept our offer and test ab105439 & ab97715in Flow Cytometry. This code will give you: 1 free PRIMARY ANTIBODY per discount code before the expiration date. To redeem this offer, please submit an Abreview forFlow Cytometryand include this code in the “Additional Comments” section so we know the Abreview is for this promotion. Please remember that submission of the Abreview is sufficient for the discount code to become active.
For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.


Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

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Question

We spoke over the telephone regarding product code ab1286 which is a mixture of two clones: 1) Isotype: IgG1, Clone:HybIgG1, Label:FITC 2) Isotype: IgG2a, Clone:HybIgG2a, Label:PE Ideally I want just clone HybIgG1 labelled with FITC. Do you stock this product?

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Answer

I think ab1264 is what you are looking for!

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Question

Is this antibody a suitable negative control for ICC applications?

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Answer

Thank you for your enquiry. To our knowledge, this antibody has yet to be tested in ICC. All tested applications are specified on Abcam product datasheets. If you decide to go ahead and purchase this product, please let us know how you get on and in return we will forward a reward of your choice, typically an Amazon gift voucher.

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