FITC Anti-Ctip2 抗体 [25B6] (ab123449)
Key features and details
- FITC Rat monoclonal [25B6] to Ctip2
- Suitable for: ICC/IF, Flow Cyt (Intra)
- Reacts with: Human
- Conjugation: FITC. Ex: 493nm, Em: 528nm
- Isotype: IgG2a
Related conjugates and formulations
製品の概要
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製品名
FITC Anti-Ctip2 antibody [25B6]
Ctip2 一次抗体 製品一覧 -
製品の詳細
FITC Rat monoclonal [25B6] to Ctip2 -
由来種
Rat -
標識
FITC. Ex: 493nm, Em: 528nm -
アプリケーション
適用あり: ICC/IF, Flow Cyt (Intra)more details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Zebrafish -
免疫原
Recombinant fragment corresponding to Human Ctip2 aa 1-150.
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ポジティブ・コントロール
- Jurkat cells.
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特記事項
Fluorochrome/Protein Ratio: at least 3 moles FITC per mole of IgG.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Product was previously marketed under the MitoSciences sub-brand.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. Avoid freeze / thaw cycle. Store In the Dark. -
バッファー
Preservative: 0.02% Sodium azide
Constituents: 98% PBS, 1% BSA -
Concentration information loading...
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精製度
Ammonium Sulphate Precipitation -
特記事項(精製)
Purity is near homogeneity as judged by SDS-PAGE. ab123449 was produced in vitro using hybridomas grown in serum-free medium, and then concentrated by ammonium sulfate precipitation. -
ポリ/モノ
モノクローナル -
クローン名
25B6 -
アイソタイプ
IgG2a -
研究分野
関連製品
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Alternative Versions
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab123449の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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ICC/IF |
Use a concentration of 0.125 - 1 µg/ml.
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Flow Cyt (Intra) |
Use a concentration of 0.125 - 1 µg/ml.
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特記事項 |
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ICC/IF
Use a concentration of 0.125 - 1 µg/ml. |
Flow Cyt (Intra)
Use a concentration of 0.125 - 1 µg/ml. |
ターゲット情報
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機能
Tumor-suppressor protein involved in T-cell lymphomas. May function on the P53-signaling pathway. May be a key regulator of both differentiation and survival during thymocyte development. Repress transcription through direct, TFCOUP2-independent binding to a GC-rich response element. -
組織特異性
Highly expressed in brain and in malignant T-cell lines derived from patients with adult T-cell leukemia/lymphoma. -
配列類似性
Contains 6 C2H2-type zinc fingers. -
細胞内局在
Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 64919 Human
- Entrez Gene: 58208 Mouse
- Omim: 606558 Human
- SwissProt: Q9C0K0 Human
- SwissProt: Q99PV8 Mouse
- Unigene: 709690 Human
- Unigene: 392694 Mouse
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別名
- ATL1 alpha antibody
- ATL1 antibody
- ATL1 beta antibody
see all
画像
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ab123449 staining CTIP2 in Jurkat cells. The cells were fixed with 80% methanol (5min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab123449 at 1/100 dilution (shown in green) and ab190573, Rabbit monoclonal to alpha Tubulin (Alexa Fluor® 647), at 1/1000 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
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ab123449 at 0.125µg/ml staining Ctip2 in Jurkat cells by Flow Cytometry (red) or an equal amount of an FITC conjugated control antibody (black) in 3% BSA block.The Jurkat cells show a clear CTIP2 specific signal
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ab123449 at 0.125µg/ml staining Ctip2 in SH-SY5Y cells by Flow Cytometry (red) or an equal amount of an FITC conjugated control antibody (black) in 3% BSA block. The SH-SY5Y cells do not show significant CTIP2 signal over the negative control signal.
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (3)
ab123449 は 3 報の論文で使用されています。
- Del Toro D et al. Structural Basis of Teneurin-Latrophilin Interaction in Repulsive Guidance of Migrating Neurons. Cell 180:323-339.e19 (2020). PubMed: 31928845
- Zhong C et al. Differential Expression of the Transcription Factor GATA3 Specifies Lineage and Functions of Innate Lymphoid Cells. Immunity 52:83-95.e4 (2020). PubMed: 31882362
- Forouzanfar F et al. HIV-1 Vpr mediates the depletion of the cellular repressor CTIP2 to counteract viral gene silencing. Sci Rep 9:13154 (2019). PubMed: 31511615