FITC Anti-CD90 / Thy1 抗体 [MRC OX-7] (ab226)

Submit a review Q&A (2)References (16)
Immunocytochemistry/ Immunofluorescence - FITC Anti-CD90 / Thy1 antibody [MRC OX-7] (ab226)
  • Flow Cytometry - FITC Anti-CD90 / Thy1 antibody [MRC OX-7] (ab226)

Key features and details

  • FITC Mouse monoclonal [MRC OX-7] to CD90 / Thy1
  • Suitable for: Flow Cyt, ICC/IF
  • Reacts with: Mouse, Rat, Rabbit, Guinea pig
  • Conjugation: FITC. Ex: 493nm, Em: 528nm
  • Isotype: IgG1

製品の概要

  • 製品名

    FITC Anti-CD90 / Thy1 antibody [MRC OX-7]
    CD90 / Thy1 一次抗体 製品一覧

  • 製品の詳細

    FITC Mouse monoclonal [MRC OX-7] to CD90 / Thy1

  • 由来種

    Mouse

  • 標識

    FITC. Ex: 493nm, Em: 528nm

  • 特異性

    Recognises the Thy-1.1 antigenic determinant which is a monomorphic determinant within rat strains but polymorphic in mice. Thus MRC OX-7 reacts with Thy-1.1 mice eg. AKR strain, but not Thy-1.2 mice eg. CBA, BALB/c.

  • アプリケーション

    適用あり: Flow Cyt, ICC/IFmore details

  • 種交差性

    交差種: Mouse, Rat, Rabbit, Guinea pig

  • 免疫原

    corresponding to CD90/ Thy1.

  • ポジティブ･コントロール

    • Flow Cyt: PC12 cells. ICC/IF: PC12 cells

  • 特記事項

    The affinity of the Fab' of MRC OX-7 for rat Thy-1 is 3 x 109m-1 and for mouse Thy-1.1 is 3 x 108m-1.

    Conjugated to Fluorescein Isothiocyanite Isomer 1 (FITC). FITC : Protein (molar ratio): 5.0 : 1.0.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab226 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Flow Cyt 1/500.

ab91356 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

 
ICC/IF 1/100.

This product gave a positive signal in PC12 cells fixed with 100% methanol (5 min)

ターゲット情報

  • 機能

    May play a role in cell-cell or cell-ligand interactions during synaptogenesis and other events in the brain.

  • 配列類似性

    Contains 1 Ig-like V-type (immunoglobulin-like) domain.

  • 細胞内局在

    Cell membrane.
  • Information by UniProt

  • 参照データベース

    • 別名

      • CD7 antibody
      • CD90 antibody
      • CD90 antigen antibody
      • CDw90 antibody
      • FLJ33325 antibody
      • MGC128895 antibody
      • T25 antibody
      • Theta antigen antibody
      • Thy 1 antibody
      • Thy 1 cell surface antigen antibody
      • Thy 1 membrane glycoprotein antibody
      • Thy 1 T cell antigen antibody
      • Thy 1.2 antibody
      • Thy-1 antigen antibody
      • Thy-1 membrane glycoprotein antibody
      • Thy1 antibody
      • Thy1 antigen antibody
      • Thy1 T cell antigen antibody
      • Thy1.1 antibody
      • Thy1.2 antibody
      • THY1_HUMAN antibody
      • Thymus cell antigen 1, theta antibody
      see all

    画像

    • Immunocytochemistry/ Immunofluorescence - FITC Anti-CD90 / Thy1 antibody [MRC OX-7] (ab226)
      Immunocytochemistry/ Immunofluorescence - FITC Anti-CD90 / Thy1 antibody [MRC OX-7] (ab226)

      ab226 staining CD90 / Thy1 in PC12 cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab226 at 1/100 dilution (shown in green) and ab195889, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 594), at 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).

      Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    • Flow Cytometry - FITC Anti-CD90 / Thy1 antibody [MRC OX-7] (ab226)
      Flow Cytometry - FITC Anti-CD90 / Thy1 antibody [MRC OX-7] (ab226)

      Overlay histogram showing PC12 cells stained with ab226 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab226, 1/500 dilution) for 30 min at 22°C.

      Isotype control antibody (black line) was Mouse IgG1 (monoclonal) FITC (ab18435) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.

      Acquisition of >5,000 events were collected using a 50 mW Blue laser (488nm) and 530/30 bandpass filter.

    参考文献 (16)

    ab226 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab226 は 16 報の論文で使用されています。

    • Tarafder S  et al. Engineered Healing of Avascular Meniscus Tears by Stem Cell Recruitment. Sci Rep 8:8150 (2018). PubMed: 29802356
    • Pak S  et al. Endoscopic Transplantation of Mesenchymal Stem Cell Sheets in Experimental Colitis in Rats. Sci Rep 8:11314 (2018). PubMed: 30054522
    • Li J  et al. Carbon monoxide releasing molecule-3 promotes the osteogenic differentiation of rat bone marrow mesenchymal stem cells by releasing carbon monoxide. Int J Mol Med 41:2297-2305 (2018). PubMed: 29393384
    • Chopra H  et al. Lapine periodontal ligament stem cells for musculoskeletal research in preclinical animal trials. J Transl Med 16:174 (2018). PubMed: 29929550
    • Sun AJ  et al. Comparison of mouse brown and white adipose-derived stem cell differentiation into pacemaker-like cells induced by TBX18 transduction. Mol Med Rep 17:7055-7064 (2018). PubMed: 29568953
    View all Publications for this product

    レビューと Q&A

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    Question

    LOT NUMBER GR22543 ORDER NUMBER xxxxx DESCRIPTION OF THE PROBLEM Positive marker CD90 shows negative SAMPLE Rabbit mesenchymal stem cell PRIMARY ANTIBODY Ab226 1:100 dilution in FACS buffer(2%FBS(hear inactivated) in PBS(pH7.2) with 0.05%NaN3)for 30min-1hr DETECTION METHOD Analysis: FACS instrument BD FACS Cailbur FACS instrument is located in a core facility? Cells gated yes/no Yes POSITIVE AND NEGATIVE CONTROLS USED Positive control N/A Negative control IgG1 (Southern biotech, FITC, Cat. 0102-02) ANTIBODY STORAGE CONDITIONS -20C SAMPLE PREPARATION FACS buffer (2%FBS(hear inactivated) in PBS(pH7.2) with 0.05%NaN3) NUMBER OF CELLS USED 5X10[5] PERMEABILIZATION STEP N/A BLOCKING CONDITIONS 1% BSA in PBS 30-1 hr at 4C SECONDARY ANTIBODY N/A HOW MANY TIMES HAVE YOU TRIED THE APPLICATION? 3

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    Answer

    Thank you for contacting us and reporting the problems you have experienced in using ab226. The details provided will enable us to investigate this case and will provide us with vital information for monitoring product quality. Having reviewed this case, I would like to offer some suggestions to help optimize the results from ab226. I would also appreciate if you can confirm some further details: 1. What washing steps were preformed? And with what buffers? 2. What volume of buffer was the cells suspended in for the primary antibody incubation? 3. Was the antibody kept in the dark at all times? 4. Was any analysis performed to detect the dead cells within the sample? I would suggest trying to use a higher concentration of primary antibody. The directions supplied with the datasheet are only guidelines and may need to be optimised for your particular experiment. I would suggest using a 1/10 dilution. It may also be worth trying a different blocking agent such as 3% BSA in PBS. Care should also be taken to keep the sample ice cold when preparing and I would suggest incubating the antibody with the cells (in the dark) for 1 hour at 4°C. Should the suggestions not improve the results, please do let me know. In the event that a product is not functioning in the species and applications cited on the product datasheet (and the problem has been reported within 6 months of purchase), we would be pleased to provide a free of charge replacement, credit note, or refund. I hope this information is helpful, and I thank you for your cooperation.

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    Question

    Further to my email below, could you also tell me if the following antibody would bind the cell surface of NIH-3T3 cells?   https://www.abcam.com/Sca1-Ly6A-E-antibody-D7-FITC-ab25031.html#ab25031_1.jpg       Good afternoon,   I am looking for FITC and PE-conjugated antibodies to us as positive controls for flow cytometry compensation set-up. I am using NIH-3T3 cells and have found a publication suggesting cell-surface expression of Thy-1. Please could you confirm if the anti-CD90 mAb below would be suitable.   https://www.abcam.com/CD90-Thy1-antibody-FITC-MRC-OX-7-FITC-ab226.html#ab226_1.jpg           Many thanks in advance,        

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    Answer

    Thank you for contacting us. We are happy to help customers find the most suitable products for their research purposes; however we are not specialized in this particular field and do not want to recommend a product that may be unsuitable. We encourage customers to consult the latest literature available through PubMed and other resources in order to find the most up-to-date information about their specific research interests. I found conflicting information about the expression of CD90 on NIH3T3 cells: No expression or expression of Thy1.2 (CD90.2). ab226 only recognizes Thy1.1 (CD90.1). I therefore suggest to use ab25672 (Anti-CD90 / Thy1 antibody [G7] (FITC)) which will bind CD90.1 and CD90.2. I could not find any reliable data on the expression of Ly6 on NIH3T3 cells either, but can confirm that ab25031 will bind to Ly6A.2 and Ly6E. I am sorry that I could not be more helpful, but I hope that the available literature in this area can provide some clarification. Please do not hesitate to contact us again with other needs or with any questions about our products.

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