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  1. Link

    fitc-cd63-antibody-ad1-ab108949.pdf

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Cardiovascular Blood Platelets
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FITC Anti-CD63 抗体 [AD1] (ab108949)

  • Datasheet
Submit a review Q&A (7)References (1)

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Abpromise

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Flow Cytometry - FITC Anti-CD63 antibody [AD1] (ab108949)

    Key features and details

    • FITC Mouse monoclonal [AD1] to CD63
    • Suitable for: Flow Cyt
    • Reacts with: Rat
    • Conjugation: FITC. Ex: 493nm, Em: 528nm
    • Isotype: IgG1

    Conjugates logo Related conjugates and formulations

    Unconjugated

    こちらの製品もご検討ください

    標識
    Product image
    FITC Conjugation Kit - Lightning-Link® (ab102884)
    一次抗体
    Product image
    Anti-CD63 antibody [EPR21151] - BSA and Azide free (ab227892)

    関連製品

    製品の概要

    • 製品名

      FITC Anti-CD63 antibody [AD1]
      CD63 一次抗体 製品一覧
    • 製品の詳細

      FITC Mouse monoclonal [AD1] to CD63
    • 由来種

      Mouse
    • 標識

      FITC. Ex: 493nm, Em: 528nm
    • 特異性

      Rat CD63 is expressed on mast cells, and studies using clone AD1, have shown that it is located near to FcER1, and that clone AD1 partially inhibits IgE-mediated histamine release
    • アプリケーション

      適用あり: Flow Cytmore details
    • 種交差性

      交差種: Rat
    • 免疫原

      Tissue, cells or virus corresponding to Rat CD63. RBL-2H3 rat basophilic leukaemia cell line.

    • 特記事項

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    製品の特性

    • 製品の状態

      Liquid
    • 保存方法

      Shipped at 4°C. Store at +4°C.
    • バッファー

      Preservative: 0.09% Sodium azide
      Constituents: PBS, 1% BSA
    • Concentration information loading...
    • 精製度

      Protein G purified
    • ポリ/モノ

      モノクローナル
    • クローン名

      AD1
    • ミエローマ

      x63-Ag8.653
    • アイソタイプ

      IgG1
    • 研究分野

      • Cardiovascular
      • Blood
      • Platelets
      • Immunology
      • Cell Type Markers
      • CD
      • Platelets
      • Tags & Cell Markers
      • Cell Type Markers
      • Tumor Associated
      • Cancer
      • Invasion/microenvironment
      • ECM
      • Cell adhesion
      • Other
      • Cancer
      • Tumor biomarkers
      • Tumor antigens

    関連製品

    • Alternative Versions

      • Anti-CD63 antibody [AD1] (ab108950)
    • Isotype control

      • FITC Mouse IgG1 [B11/6] - Isotype Control (ab91356)

    アプリケーション

    The Abpromise guarantee

    Abpromise保証は、 次のテスト済みアプリケーションにおけるab108949の使用に適用されます

    アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

    アプリケーション Abreviews 特記事項
    Flow Cyt
    Use at an assay dependent concentration.

    ab91356 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

     

    特記事項
    Flow Cyt
    Use at an assay dependent concentration.

    ab91356 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

     

    ターゲット情報

    • 機能

      This antigen is associated with early stages of melanoma tumor progression. May play a role in growth regulation.
    • 組織特異性

      Dysplastic nevi, radial growth phase primary melanomas, hematopoietic cells, tissue macrophages.
    • 配列類似性

      Belongs to the tetraspanin (TM4SF) family.
    • 細胞内局在

      Cell membrane. Lysosome membrane. Late endosome membrane. Also found in Weibel-Palade bodies of endothelial cells. Located in platelet dense granules.
    • Target information above from: UniProt accession P08962 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • 参照データベース

      • Entrez Gene: 29186 Rat
      • SwissProt: P28648 Rat
      • Unigene: 11068 Rat
      • 別名

        • Lysosomal associated membrane protein 3 antibody
        • CD 63 antibody
        • CD63 antibody
        • CD63 antigen (melanoma 1 antigen) antibody
        • CD63 antigen antibody
        • CD63 antigen melanoma 1 antigen antibody
        • CD63 molecule antibody
        • CD63_HUMAN antibody
        • gp55 antibody
        • Granulophysin antibody
        • LAMP 3 antibody
        • LAMP-3 antibody
        • LAMP3 antibody
        • LIMP antibody
        • Lysosomal-associated membrane protein 3 antibody
        • Lysosome associated membrane glycoprotein 3 antibody
        • Lysosome-associated membrane glycoprotein 3 antibody
        • Mast cell antigen AD1 antibody
        • ME491 antibody
        • Melanoma 1 antigen antibody
        • Melanoma associated antigen ME491 antibody
        • Melanoma associated antigen MLA1 antibody
        • Melanoma-associated antigen ME491 antibody
        • MGC72893 antibody
        • MLA 1 antibody
        • MLA1 antibody
        • NGA antibody
        • Ocular melanoma associated antigen antibody
        • Ocular melanoma-associated antigen antibody
        • OMA81H antibody
        • PTLGP40 antibody
        • Tetraspanin 30 antibody
        • Tetraspanin-30 antibody
        • Tspan 30 antibody
        • Tspan-30 antibody
        • TSPAN30 antibody
        see all

      画像

      • Flow Cytometry - FITC Anti-CD63 antibody [AD1] (ab108949)
        Flow Cytometry - FITC Anti-CD63 antibody [AD1] (ab108949)
        ab108949 detecting CD63 in Rat peripheral blood platelets by Flow Cytometric analysis.

      プロトコール

      • Flow cytometry protocols

      Click here to view the general protocols

      データシートおよび資料

      • Datasheet download

        Download

      参考文献 (1)

      ab108949 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

      ab108949 は 1 報の論文で使用されています。

      • Kitani S  et al. A cell surface glycoprotein of rat basophilic leukemia cells close to the high affinity IgE receptor (Fc epsilon RI). Similarity to human melanoma differentiation antigen ME491. J Biol Chem 266:1903-9 (1991). WB, IP ; Rat . PubMed: 1703158

      レビューと Q&A

      Show All レビュー Q&A
      レビューを送る 質問を送る

      1-7 of 7 Abreviews or Q&A

      Question

      The customer details are:


      Read More

      Abcam community

      Verified customer

      Asked on Jun 27 2012

      Answer

      DISCOUNT CODE: XXXXXXXX
      Expiration date: 27-10-2012
      I am very pleased to hear you would like to accept our offer and test ab108949 in Functional assays. This code will give you: 1 free [PRIMARY ANTIBODY/PROTEIN] OR VALUE OFF OF ORDER before the expiration date. To redeem this offer, please submit an Abreview for [Functional assay] and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. Please remember that submission of the Abreview is sufficient for the discount code to become active.
      For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.
      Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.
      Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.
      The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

      Read More

      Abcam Scientific Support

      Answered on Jun 27 2012

      Question

      Thank you for this reply.
      The customer is interested in your suggestion.
      Please issue the discount code. Kindly recommend the dilution (if it is possible) of the starting point for the calibration of this functional study.
      Thank you.

      Best Regards,

      Read More

      Abcam community

      Verified customer

      Asked on Jun 26 2012

      Answer

      Could you provide customer details?
      Thanks!

      Read More

      Abcam Scientific Support

      Answered on Jun 26 2012

      Question

      Product code: 108949
      Inquiry: The customer wonders if there is a chance that ab108949 will be suitable for functional assay: this antibody should block CD63 activity in vivo. The location of the binding of the Ab to CD63 will be tested further by IHC staining. Please advise. Thank you very much for your assistance and cooperation. Have a nice week.

      Read More

      Abcam community

      Verified customer

      Asked on Jun 25 2012

      Answer

      Thank you very much for your interest in ab108949.
      To our knowledge, ab108949 has not been tested in Functional assays. Therefore, I can offer a discount off a future purchase if you buy ab108949 now, test it in (Functional assays) and submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of: 1 free (PRIMARY ANTIBODY/PROTEIN) OR VALUE OFF OF NEXT ORDER.
      If you are interested in this offer, please follow these steps:
      1. Reply to this e-mail to let me know that you would like to proceed and test ab108949 in (Functional assays). I will then send a discount code. This code must be issued before purchasing ab108949 so please wait for my reply before ordering.
      2. Purchase ab108949 either by phone, fax, or online (www.abcam.com).
      3. Test it in Functional assays.
      4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews.
      5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for any [PRIMARY ANTIBODY /PROTEIN /PRODUCT] ordered and the discount code is valid for 4 months after issue.
      Please remember that submission of the Abreview is sufficient for the discount code to become active.
      We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if ab108949 turns out to be unsuitable for (Functional assays), you will still receive the discount on your next purchase after your Abreview has been submitted.
      Please let me know if you have any questions about this offer and I would be happy to help you further.
      The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.

      Read More

      Abcam Scientific Support

      Answered on Jun 25 2012

      Question

      We have purchased some antibodies against rat platelets:

      Primary:

      ab108949 - mouse monoclonal anti CD63, FITC labeled
      (ab106163 as isotype control)

      ab54427 - mouse monoclonal anti CD62P
      (ab91353 as the isotype control, as a secondary antibody)

      ab95780 anti CD42d armenian hamster antibody
      (ab18479 as an isotype control, for which we are using APC-conjugated Goat Anti-Hamster IgG from Leinco).

      We are ibodies.doing flow cytometry experiments on fixed whole blood, and fixed platelet-rich plasma. The problem is that the samples with the isotype control show the same fluorescence as the samples with the primary ant We have tried a number of different fixing and staining protocols, with and without washing, but until now had no success. What I am wondering about is, how reliable are these primary antibodies for the use with rat platelets?

      Some help would be greatly appreciated.

      Read More

      Abcam community

      Verified customer

      Asked on Jun 05 2012

      Answer

      Thank you for your reply.

      I am sorry that you have been having issues with some of our antibodies in Flow Cytometry. Under our Abpromise we guarantee that ab108949, ab54427 & ab95780 should work in flow cytometry on rat samples. If we cannot resolve the issue you are having with the antibody then I would be happy to either send a replacement antibody or to process a refund.

      To be able to help you further in this matter, would you be able to send me a copy of the protocol that you have been using, along with any modifications that you have tried, to see if there is anything I can suggest.

      I look forward to your reply and helping resolve this matter.

      Read More

      Abcam Scientific Support

      Answered on Jun 05 2012

      Question

      Yes, it is correct. ************does the purchasing in this group usually. I am a visiting professor here.

      Feel free to contact him to double-check, he is the one who gave me the customer ID.

      Read More

      Abcam community

      Verified customer

      Asked on Mar 22 2012

      Answer

      Thank you for your reply.

      I have sent the quote for the products requested to the email address we have on file for **********.

      When you are ready to convert the quote to an order, please contact our customer service team on 1-888-772-2226 or email to mailto:us.orders@abcam.com.

      If there is anything else I can help you with, please let me know.

      Read More

      Abcam Scientific Support

      Answered on Mar 22 2012

      Question

      Concerning the quote, the customer number is**********

      Read More

      Abcam community

      Verified customer

      Asked on Mar 22 2012

      Answer

      Thank you for your reply.

      Sorry to keep asking you questions, but the customer ID you gave me is for a Dr Alejandro Gomez, is that correct. I ask as its does not mentioned Bone Interface Group in their address that we have on file and I just wanted to make sure that the quote went to the correct person.

      Thank you for your patience, and I look forward to your reply.

      Read More

      Abcam Scientific Support

      Answered on Mar 22 2012

      Question

      I selected the following antibodies from the ABcam catalog:

      Primary:

      Anti-CD63 antibody [AD1] (FITC)

      ab108949

      $337.00



      Anti-CD62P antibody [Psel.KO.2.7]

      ab54427

      $337.00



      Anti-CD42d antibody [1C2]

      ab95780

      $337.00



      Secondary:


      Goat anti-Mouse IgG H&L (Cy5.5 ®)

      ab6947

      $185.00



      Goat anti-Mouse IgG1 heavy chain (FITC)

      ab97239

      $165.00



      Goat polyclonal to Armenian Hamster (TR)

      ab5743

      $165.00



      Isotype controls:


      MOUSE IgG1 antibody [MOPC-21] (FITC)

      ab106163

      $325.00



      Mouse IgG1 [ICIGG1] - Isotype Control

      ab91353

      $315.00



      Armenian Hamster IgG monoclonal [HTK888] - Isotype control

      ab18479

      $325.00



      Two questions:

      For the secondary antibody, will the first one (ab6947) work against ab54427, or do we need to buy the second one (ab97239)? One is against IgG, the other one is against IgG1. ab54427 is IgG1.

      If you could double-check the selection for compatibility (e.g., correct isotype controls) and then ask the sales department to offer us a quote, that would be great.

      Read More

      Abcam community

      Verified customer

      Asked on Mar 21 2012

      Answer

      Thank you for your reply.

      The antibodies you selected look good to me. To generate a quote we do need to have a shipping address and in your last email, you mentioned that you were at the University of Toronto at the moment. Could you please send me your current email address and I will generate the quote.

      I look forward to your reply.

      Read More

      Abcam Scientific Support

      Answered on Mar 21 2012

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com

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