FITC Anti-CD43 抗体 [MEM-59] (ab21853)
![Flow Cytometry - FITC Anti-CD43 antibody [MEM-59] (ab21853)](https://www.abcam.co.jp/ps/products/21/ab21853/Images/ab21853-278866-anti-cd43-antibody-mem-59-fitc-flow-cytometry.jpg "Flow Cytometry - FITC Anti-CD43 antibody [MEM-59] (ab21853)")
Key features and details
- FITC Mouse monoclonal [MEM-59] to CD43
- Suitable for: Flow Cyt
- Reacts with: Human
- Conjugation: FITC. Ex: 493nm, Em: 528nm
- Isotype: IgG1
Related conjugates and formulations
製品の概要
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製品名
FITC Anti-CD43 antibody [MEM-59]
CD43 一次抗体 製品一覧 -
製品の詳細
FITC Mouse monoclonal [MEM-59] to CD43 -
由来種
Mouse -
標識
FITC. Ex: 493nm, Em: 528nm -
アプリケーション
適用あり: Flow Cytmore details -
種交差性
交差種: Human -
免疫原
Tissue, cells or virus corresponding to Human CD43. T lymphocytes
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特記事項
The purified antibody is conjugated with Fluorescein isothiocyanate (FITC) under optimum conditions. The reagent is free of unconjugated FITC and adjusted for direct use.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. -
バッファー
pH: 7.40
Preservative: 0.097% Sodium azide
Constituents: PBS, BSA -
Concentration information loading... -
精製度
Protein G purified -
ポリ/モノ
モノクローナル -
クローン名
MEM-59 -
アイソタイプ
IgG1 -
研究分野
関連製品
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Alternative Versions
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab21853の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| Flow Cyt |
Use 1µl for 106 cells.
ab91356 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
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| 特記事項 |
|---|
|
Flow Cyt
Use 1µl for 106 cells. ab91356 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
|
ターゲット情報
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機能
One of the major glycoproteins of thymocytes and T lymphocytes. Plays a role in the physicochemical properties of the T-cell surface and in lectin binding. Presents carbohydrate ligands to selectins. Has an extended rodlike structure that could protrude above the glycocalyx of the cell and allow multiple glycan chains to be accessible for binding. Is a counter receptor for SN/Siglec-1 (By similarity). During T-cell activation is actively removed from the T-cell-APC (antigen-presenting cell) contact site thus suggesting a negative regulatory role in adaptive immune response. -
組織特異性
Cell surface of thymocytes, T-lymphocytes, neutrophils, plasma cells and myelomas. -
翻訳後修飾
Glycosylated; has a high content of sialic acid and O-linked carbohydrate structures. -
細胞内局在
Membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 6693 Human
- Omim: 182160 Human
- SwissProt: P16150 Human
- Unigene: 632188 Human
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別名
- CD 43 antibody
- CD43 antibody
- CD43 antigen antibody
see all
画像
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Flow Cytometry - FITC Anti-CD43 antibody [MEM-59] (ab21853)Flow Cytometry analysis of human peripheral blood cells labeling CD43 with Anti-CD43 antibody [MEM-59] (FITC) (ab21853).
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![Flow Cytometry - FITC Anti-CD43 antibody [MEM-59] (ab21853)](https://www.abcam.co.jp/ps/products/21/ab21853/Images/ab21853-31178-anti-cd43-antibody-mem-59-fitc-flow-cytometry.jpg)
Flow Cytometry - FITC Anti-CD43 antibody [MEM-59] (ab21853)Overlay histogram showing peripheral blood lymphocytes stained with ab21853(red line). The cells incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab21853, 0.5 µg/1x106 cells) for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 FITC (2 µg/1x106 cells ) for 30 min at 22°C. Acquisition of >5,000 events was performed.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (2)
ab21853 は 2 報の論文で使用されています。
- Tingling JD et al. CD24 expression identifies teratogen-sensitive fetal neural stem cell subpopulations: evidence from developmental ethanol exposure and orthotopic cell transfer models. PLoS One 8:e69560 (2013). Flow Cyt ; Mouse . PubMed: 23894503
- Stracker TH et al. The carboxy terminus of NBS1 is required for induction of apoptosis by the MRE11 complex. Nature 447:218-21 (2007). WB ; Mouse . PubMed: 17429352