Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online

FITC Anti-CD34 抗体 [4H11[APG]] (ab18227)

Submit a review Q&A (1)References (8)
Flow Cytometry - FITC Anti-CD34 antibody [4H11[APG]] (ab18227)

    Key features and details

    • FITC Mouse monoclonal [4H11[APG]] to CD34
    • Suitable for: Flow Cyt
    • Reacts with: Human
    • Conjugation: FITC. Ex: 493nm, Em: 528nm
    • Isotype: IgG1

    製品の概要

    • 製品名

      FITC Anti-CD34 antibody [4H11[APG]]
      CD34 一次抗体 製品一覧

    • 製品の詳細

      FITC Mouse monoclonal [4H11[APG]] to CD34

    • 由来種

      Mouse

    • 標識

      FITC. Ex: 493nm, Em: 528nm

    • アプリケーション

      適用あり: Flow Cytmore details

    • 種交差性

      交差種: Human

    • 免疫原

      Tissue, cells or virus corresponding to Human CD34. Permanent human cell line derived from peripheral leucocytes of a patient suffering from chronic myeloid leukaemia

    • エピトープ

      Class III epitope on CD34 antigen.

    • ポジティブ･コントロール

      • Human blood cells.

    • 特記事項

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    製品の特性

    アプリケーション

    The Abpromise guarantee

    Abpromise保証は、 次のテスト済みアプリケーションにおけるab18227の使用に適用されます

    アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

    アプリケーション Abreviews 特記事項
    Flow Cyt
    特記事項
    追加情報
    Flow Cyt: Use 20 µl reagent / 100 µl sample.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    ターゲット情報

    • 機能

      Possible adhesion molecule with a role in early hematopoiesis by mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells. Could act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components. Presents carbohydrate ligands to selectins.

    • 組織特異性

      Selectively expressed on hematopoietic progenitor cells and the small vessel endothelium of a variety of tissues.

    • 配列類似性

      Belongs to the CD34 family.

    • 発生段階

      On early hematopoietic progenitor cells.

    • 翻訳後修飾

      Highly glycosylated.
      Phosphorylated on serine residues by PKC.

    • 細胞内局在

      Membrane.
    • Information by UniProt

    • 参照データベース

      • 別名

        • CD34 antibody
        • CD34 antigen antibody
        • CD34 molecule antibody
        • CD34_HUMAN antibody
        • Cluster designation 34 antibody
        • cluster of differentiation 34 antibody
        • Hematopoietic progenitor cell antigen CD34 antibody
        • HPCA1 antibody
        • Mucosialin antibody
        • OTTHUMP00000034733 antibody
        • OTTHUMP00000034734 antibody
        see all

      画像

      • Flow Cytometry - FITC Anti-CD34 antibody [4H11[APG]] (ab18227)
        Flow Cytometry - FITC Anti-CD34 antibody [4H11[APG]] (ab18227)

        Surface staining of Kg-1a human acute myelogenous leukemia cell line with anti-human CD34 (4H11[APG]) FITC (ab18227). Total viable cells were used for analysis.

        ab18227 was used at a recommended dilution of 2 μg/ml.

      参考文献 (8)

      ab18227 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

      ab18227 は 8 報の論文で使用されています。

      • Karakas N  et al. Neurons from human mesenchymal stem cells display both spontaneous and stimuli responsive activity. PLoS One 15:e0228510 (2020). PubMed: 32407317
      • Chen Y  et al. MSC-Secreted Exosomal H19 Promotes Trophoblast Cell Invasion and Migration by Downregulating let-7b and Upregulating FOXO1. Mol Ther Nucleic Acids 19:1237-1249 (2020). PubMed: 32069774
      • Meng Q & Qiu B Exosomal MicroRNA-320a Derived From Mesenchymal Stem Cells Regulates Rheumatoid Arthritis Fibroblast-Like Synoviocyte Activation by Suppressing CXCL9 Expression. Front Physiol 11:441 (2020). PubMed: 32528301
      • Cao G  et al. Human Adipose-Derived Mesenchymal Stem Cells-Derived Exosomal microRNA-19b Promotes the Healing of Skin Wounds Through Modulation of the CCL1/TGF-ß Signaling Axis. Clin Cosmet Investig Dermatol 13:957-971 (2020). PubMed: 33364805
      • Huang N  et al. Neuroprotective effect of tanshinone IIA-incubated mesenchymal stem cells on Aß25-35-induced neuroinflammation. Behav Brain Res 365:48-55 (2019). PubMed: 30831140
      • Wu YT  et al. A novel and feasible way to cultivate and purify endothelial progenitor cells from bone marrow of children with congenital heart diseases. Chin Med J (Engl) 125:1903-7 (2012). Flow Cyt ; Human . PubMed: 22884051
      • Sampat SR  et al. Growth factor priming of synovium-derived stem cells for cartilage tissue engineering. Tissue Eng Part A 17:2259-65 (2011). Flow Cyt ; Cow . PubMed: 21542714
      • Gong Z & Niklason LE Small-diameter human vessel wall engineered from bone marrow-derived mesenchymal stem cells (hMSCs). FASEB J 22:1635-48 (2008). Flow Cyt ; Human . PubMed: 18199698

      レビューと Q&A

      Show All レビュー Q&A
      レビューを送る 質問を送る

      Question

      What is the IgG concentration of ab18227? I want to match the concentration of an isotype control for flow cytometry.

      Read More
      Answer

      Thank you for contacting us. The concentration of ab18227, anti-CD34, has been determined and is 50 ug/ml for the lot that you have. So, 20 ul contains 1 ug.

      Abcam also carries other products essential for Flow Cytometry such as isotype controls. If testing live cells we also recommend cell viability controls such as 7-AAD or Propidium Iodide. Propidium Iodide (PI) binds to double-stranded DNA. PI cannot cross intact plasma membrane and therefore will only be present in DNA of cells where the plasma membrane has been compromised/ permeabilized. We do carry PI and offer a 7-AAD Cell-Mediated Cytotoxicity Assay Kit.

      Propidium Iodide: https://www.abcam.com/Propidium-Iodide-ab14083.html

      7-AAD Kit: https://www.abcam.com/7-AADCFSE-Cytotoxicity-Assay-Kit-ab133073.html

      I hope this information has been of help to you. If you have any further questions, please do not hesitate to ask.

      Read More

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com