FITC Anti-Biotin 抗体 (ab6650)

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Key features and details

  • FITC Goat polyclonal to Biotin
  • Suitable for: Flow Cyt, ICC/IF
  • Reacts with: Species independent
  • Conjugation: FITC. Ex: 493nm, Em: 528nm
  • Isotype: IgG

製品の概要

  • 製品名

    FITC Anti-Biotin antibody
    Biotin 一次抗体 製品一覧

  • 製品の詳細

    FITC Goat polyclonal to Biotin

  • 由来種

    Goat

  • 標識

    FITC. Ex: 493nm, Em: 528nm

  • アプリケーション

    適用あり: Flow Cyt, ICC/IFmore details

  • 種交差性

    交差種: Species independent

  • 免疫原

    Chemical/ Small Molecule conjugated to KLH

  • 特記事項

    Fluorescein isothiocyanate (FITC) (Molecular Weight 390 daltons) Absorption Wavelength: 495 nm Emission Wavelength: 528 nm Fluorochrome/Protein Ratio: 2.7 moles FITC per mole of Goat IgG

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab6650 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Flow Cyt Use at an assay dependent concentration.

ab37394 - Goat polyclonal IgG, is suitable for use as an isotype control with this antibody.
ICC/IF 1/500 - 1/2500.

ターゲット情報

  • 関連性

    Biotin is a water soluble vitamin, generally classified as a B complex vitamin, also called vitamin B4. After the initial discovery of biotin, nearly forty years of research were required to establish it as a vitamin. Biotin is required by all organisms but can only be synthesized by bacteria, yeasts, molds, algae, and some plant species. Biotin is required as prosthetic group of enzymes involved in incorporation of carbon dioxide into organic compounds. Biotin has a MW of 244 Da.

  • 別名

    • Vitamin B4 antibody

参考文献 (2)

ab6650 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab6650 は 2 報の論文で使用されています。

  • Link J  et al. Transient and Partial Nuclear Lamina Disruption Promotes Chromosome Movement in Early Meiotic Prophase. Dev Cell 45:212-225.e7 (2018). PubMed: 29689196
  • Baratta JL  et al. Cellular organization of normal mouse liver: a histological, quantitative immunocytochemical, and fine structural analysis. Histochem Cell Biol 131:713-26 (2009). IHC-FoFr . PubMed: 19255771

レビューと Q&A

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1-2 of 2 Abreviews or Q&A

Question

Hello, Can you tell me how many dyes (fluorescein) are attached per molecule of anti-biotin antibody? Waiting for your reply and thanking you in advance. Cheers.

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Answer

The molar ratios of dye-to-protein are varied batch to batch. The general acceptance criteria for this product is 2-5:1

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Question

Have you used AB6650 (Goat anti-biotin Fluorescein conjugate) for membrane-based detection of Biotinylated nucleic acids on charged nylon membranes (specifically Gene-screen plus)? If so, what membrane blocking reagents have been tested & how did they work? (i.e best, 2nd best etc.)

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Answer

To our knowledge this antibody has yet to be tested in this application. We would welcome any feedback in this respect from researchers who have tried using this product.

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