FITC Anti-Biotin 抗体 (ab6650)

Submit a review Q&A (2)References (2)

Key features and details

  • FITC Goat polyclonal to Biotin
  • Suitable for: Flow Cyt, ICC/IF
  • Reacts with: Species independent
  • Conjugation: FITC. Ex: 493nm, Em: 528nm
  • Isotype: IgG

製品の概要

  • 製品名

    FITC Anti-Biotin antibody
    Biotin 一次抗体 製品一覧

  • 製品の詳細

    FITC Goat polyclonal to Biotin

  • 由来種

    Goat

  • 標識

    FITC. Ex: 493nm, Em: 528nm

  • アプリケーション

    適用あり: Flow Cyt, ICC/IFmore details

  • 種交差性

    交差種: Species independent

  • 免疫原

    Chemical/ Small Molecule conjugated to KLH

  • 特記事項

    Fluorescein isothiocyanate (FITC) (Molecular Weight 390 daltons) Absorption Wavelength: 495 nm Emission Wavelength: 528 nm Fluorochrome/Protein Ratio: 2.7 moles FITC per mole of Goat IgG

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

関連製品

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab6650の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
Flow Cyt
Use at an assay dependent concentration.

ab37394 - Goat polyclonal IgG, is suitable for use as an isotype control with this antibody.
ICC/IF
1/500 - 1/2500.
特記事項
Flow Cyt
Use at an assay dependent concentration.

ab37394 - Goat polyclonal IgG, is suitable for use as an isotype control with this antibody.
ICC/IF
1/500 - 1/2500.

ターゲット情報

  • 関連性

    Biotin is a water soluble vitamin, generally classified as a B complex vitamin, also called vitamin B4. After the initial discovery of biotin, nearly forty years of research were required to establish it as a vitamin. Biotin is required by all organisms but can only be synthesized by bacteria, yeasts, molds, algae, and some plant species. Biotin is required as prosthetic group of enzymes involved in incorporation of carbon dioxide into organic compounds. Biotin has a MW of 244 Da.

  • 別名

    • Vitamin B4 antibody

参考文献 (2)

ab6650 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab6650 は 2 報の論文で使用されています。

  • Link J  et al. Transient and Partial Nuclear Lamina Disruption Promotes Chromosome Movement in Early Meiotic Prophase. Dev Cell 45:212-225.e7 (2018). PubMed: 29689196
  • Baratta JL  et al. Cellular organization of normal mouse liver: a histological, quantitative immunocytochemical, and fine structural analysis. Histochem Cell Biol 131:713-26 (2009). IHC-FoFr . PubMed: 19255771

レビューと Q&A

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1-2 of 2 Abreviews or Q&A

Question

Hello, Can you tell me how many dyes (fluorescein) are attached per molecule of anti-biotin antibody? Waiting for your reply and thanking you in advance. Cheers.

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Answer

The molar ratios of dye-to-protein are varied batch to batch. The general acceptance criteria for this product is 2-5:1

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Question

Have you used AB6650 (Goat anti-biotin Fluorescein conjugate) for membrane-based detection of Biotinylated nucleic acids on charged nylon membranes (specifically Gene-screen plus)? If so, what membrane blocking reagents have been tested & how did they work? (i.e best, 2nd best etc.)

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Answer

To our knowledge this antibody has yet to be tested in this application. We would welcome any feedback in this respect from researchers who have tried using this product.

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