FITC Anti-beta Actin 抗体 [AC-15] (ab6277)
Key features and details
- FITC Mouse monoclonal [AC-15] to beta Actin
- Suitable for: ICC/IF
- Reacts with: Mouse, Rat, Sheep, Rabbit, Chicken, Guinea pig, Cow, Cat, Dog, Human, Pig, Drosophila melanogaster, Fish
- Conjugation: FITC. Ex: 493nm, Em: 528nm
- Isotype: IgG1
製品の概要
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製品名
FITC Anti-beta Actin antibody [AC-15]
beta Actin 一次抗体 製品一覧 -
製品の詳細
FITC Mouse monoclonal [AC-15] to beta Actin -
由来種
Mouse -
標識
FITC. Ex: 493nm, Em: 528nm -
アプリケーション
適用あり: ICC/IFmore details -
種交差性
交差種: Mouse, Rat, Sheep, Rabbit, Chicken, Guinea pig, Cow, Cat, Dog, Human, Pig, Drosophila melanogaster, Fish -
免疫原
Synthetic peptide:
DDDIAALVIDNGSGL
conjugated to KLH, corresponding to amino acids 1-14 of Xenopus laevis Actin (beta). -
エピトープ
The N-terminal of the beta isoform of actin -
ポジティブ・コントロール
- Cultured human or chicken fibroblasts. Molecular weight of beta actin is 42Kd.
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特記事項
Immunofluoresence staining of chicken gizzard ultra-thin sections labels the dense bodies, longitudinal channels and membrane associated dense-plaque.Useful for direct immunofluoresence labelling of the cytoskeleton in cultured cells
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. -
バッファー
pH: 7.40
Preservative: 0.1% Sodium azide
Constituents: 1% BSA, PBS -
Concentration information loading...
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精製度
IgG fraction -
特記事項(精製)
Purified Immunoglobulin -
一次抗体 備考
Useful for direct immunofluoresence labelling of the cytoskeleton in cultured cells -
ポリ/モノ
モノクローナル -
クローン名
AC-15 -
アイソタイプ
IgG1 -
研究分野
関連製品
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Alternative Versions
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Isotype control
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Recombinant Protein
アプリケーション
Our Abpromise guarantee covers the use of ab6277 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
アプリケーション | Abreviews | 特記事項 |
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ICC/IF | Use at an assay dependent concentration. |
ターゲット情報
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機能
Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. -
関連疾患
Defects in ACTB are a cause of dystonia juvenile-onset (DYTJ) [MIM:607371]. DYTJ is a form of dystonia with juvenile onset. Dystonia is defined by the presence of sustained involuntary muscle contraction, often leading to abnormal postures. DYTJ patients manifest progressive, generalized, dopa-unresponsive dystonia, developmental malformations and sensory hearing loss. -
配列類似性
Belongs to the actin family. -
翻訳後修飾
ISGylated. -
細胞内局在
Cytoplasm > cytoskeleton. Localized in cytoplasmic mRNP granules containing untranslated mRNAs. - Information by UniProt
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参照データベース
- Entrez Gene: 396526 Chicken
- Entrez Gene: 280979 Cow
- Entrez Gene: 403580 Dog
- Entrez Gene: 60 Human
- Entrez Gene: 11461 Mouse
- Entrez Gene: 414396 Pig
- Entrez Gene: 100009272 Rabbit
- Entrez Gene: 81822 Rat
see all -
別名
- A26C1A antibody
- A26C1B antibody
- ACTB antibody
see all
画像
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ab6277 at 1/100 dilution staining beta Actin in rat brain endothelial cells by Immunofluorescence.
30 min blocking with TBS-T / 5% BSA
1 hr incubation with ab6277 (TBS-T / 5% BSA)
1 minute nuclear counterstaining with DAPIMagnification x20
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Immunocytochemistry/ Immunofluorescence analysis of chicken fibroblast cells labeling beta Actin with ab6277 at 1/300 dilution. The cells were fixed and permeabilized with cold methanol followed by cold acetone. Fixed cells were stained with 1/300 Anti-beta Actin antibody [AC-15] (FITC) (ab6277).
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ab6277 at 1/100 dilution staining beta Actin in rat brain endothelial cells by Immunofluorescence.
30 min blocking with TBS-T / 5% BSA
1 hr incubation with ab6277 (TBS-T / 5% BSA)
1 minute nuclear counterstaining with DAPIMagnification x40 no deconvolution.
参考文献 (17)
ab6277 は 17 報の論文で使用されています。
- Cagnetta R et al. Noncanonical Modulation of the eIF2 Pathway Controls an Increase in Local Translation during Neural Wiring. Mol Cell 73:474-489.e5 (2019). PubMed: 30595434
- Réu P et al. A 61% lighter cell culture dish to reduce plastic waste. PLoS One 14:e0216251 (2019). PubMed: 31039189
- Cagnetta R et al. Rapid Cue-Specific Remodeling of the Nascent Axonal Proteome. Neuron 99:29-46.e4 (2018). PubMed: 30008298
- Olthoff JT et al. Loss of peroxiredoxin-2 exacerbates eccentric contraction-induced force loss in dystrophin-deficient muscle. Nat Commun 9:5104 (2018). PubMed: 30504831
- Llewellyn KJ et al. A Fine Balance of Dietary Lipids Improves Pathology of a Murine Model of VCP-Associated Multisystem Proteinopathy. PLoS One 10:e0131995 (2015). WB . PubMed: 26134519