FITC Anti-beta Actin 抗体 [AC-15] (ab6277)
![Immunocytochemistry/ Immunofluorescence - FITC Anti-beta Actin antibody [AC-15] (ab6277)](https://www.abcam.co.jp/ps/products/6/ab6277/Images/ab6277-12195-anti-beta-actin-antibody-ac-15-fitc-immunofluorescence.jpg "Immunocytochemistry/ Immunofluorescence - FITC Anti-beta Actin antibody [AC-15] (ab6277)")
Key features and details
- FITC Mouse monoclonal [AC-15] to beta Actin
- Suitable for: ICC/IF
- Reacts with: Rat, Chicken
- Conjugation: FITC. Ex: 493nm, Em: 528nm
- Isotype: IgG1
Related conjugates and formulations
製品の概要
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製品名
FITC Anti-beta Actin antibody [AC-15]
beta Actin 一次抗体 製品一覧 -
製品の詳細
FITC Mouse monoclonal [AC-15] to beta Actin -
由来種
Mouse -
標識
FITC. Ex: 493nm, Em: 528nm -
アプリケーション
適用あり: ICC/IFmore details -
種交差性
交差種: Rat, Chicken -
免疫原
Synthetic peptide:
DDDIAALVIDNGSGL
conjugated to KLH, corresponding to amino acids 1-14 of Xenopus laevis Actin (beta). -
エピトープ
The N-terminal of the beta isoform of actin -
ポジティブ･コントロール
- Cultured human or chicken fibroblasts. Molecular weight of beta actin is 42Kd.
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特記事項
Immunofluoresence staining of chicken gizzard ultra-thin sections labels the dense bodies, longitudinal channels and membrane associated dense-plaque.Useful for direct immunofluoresence labelling of the cytoskeleton in cultured cells
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. -
バッファー
pH: 7.40
Preservative: 0.1% Sodium azide
Constituents: 1% BSA, PBS -
Concentration information loading... -
精製度
IgG fraction -
特記事項（精製）
Purified Immunoglobulin -
一次抗体 備考
Useful for direct immunofluoresence labelling of the cytoskeleton in cultured cells -
ポリ/モノ
モノクローナル -
クローン名
AC-15 -
アイソタイプ
IgG1 -
研究分野
関連製品
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Alternative Versions
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab6277の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ICC/IF | (1) |
Use at an assay dependent concentration.
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| 特記事項 |
|---|
|
ICC/IF
Use at an assay dependent concentration. |
ターゲット情報
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機能
Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. -
関連疾患
Defects in ACTB are a cause of dystonia juvenile-onset (DYTJ) [MIM:607371]. DYTJ is a form of dystonia with juvenile onset. Dystonia is defined by the presence of sustained involuntary muscle contraction, often leading to abnormal postures. DYTJ patients manifest progressive, generalized, dopa-unresponsive dystonia, developmental malformations and sensory hearing loss. -
配列類似性
Belongs to the actin family. -
翻訳後修飾
ISGylated. -
細胞内局在
Cytoplasm > cytoskeleton. Localized in cytoplasmic mRNP granules containing untranslated mRNAs. - Information by UniProt
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参照データベース
- Entrez Gene: 396526 Chicken
- Entrez Gene: 81822 Rat
- SwissProt: P60706 Chicken
- SwissProt: P60711 Rat
- Unigene: 94978 Rat
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別名
- A26C1A antibody
- A26C1B antibody
- ACTB antibody
see all
画像
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Immunocytochemistry/ Immunofluorescence - FITC Anti-beta Actin antibody [AC-15] (ab6277)
ab6277 at 1/100 dilution staining beta Actin in rat brain endothelial cells by Immunofluorescence.
30 min blocking with TBS-T / 5% BSA
1 hr incubation with ab6277 (TBS-T / 5% BSA)
1 minute nuclear counterstaining with DAPIMagnification x20
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![Immunocytochemistry/ Immunofluorescence - FITC Anti-beta Actin antibody [AC-15] (ab6277)](https://www.abcam.co.jp/ps/products/6/ab6277/Images/ab6277-274544-anti-beta-actin-antibody-ac-15-fitc-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - FITC Anti-beta Actin antibody [AC-15] (ab6277)Immunocytochemistry/ Immunofluorescence analysis of chicken fibroblast cells labeling beta Actin with ab6277 at 1/300 dilution. The cells were fixed and permeabilized with cold methanol followed by cold acetone. Fixed cells were stained with 1/300 Anti-beta Actin antibody [AC-15] (FITC) (ab6277).
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![Immunocytochemistry/ Immunofluorescence - FITC Anti-beta Actin antibody [AC-15] (ab6277)](https://www.abcam.co.jp/ps/products/6/ab6277/Images/ab6277-12194-anti-beta-actin-antibody-ac-15-fitc-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - FITC Anti-beta Actin antibody [AC-15] (ab6277)ab6277 at 1/100 dilution staining beta Actin in rat brain endothelial cells by Immunofluorescence.
30 min blocking with TBS-T / 5% BSA
1 hr incubation with ab6277 (TBS-T / 5% BSA)
1 minute nuclear counterstaining with DAPIMagnification x40 no deconvolution.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (18)
ab6277 は 18 報の論文で使用されています。
- Cagnetta R et al. Noncanonical Modulation of the eIF2 Pathway Controls an Increase in Local Translation during Neural Wiring. Mol Cell 73:474-489.e5 (2019). PubMed: 30595434
- Réu P et al. A 61% lighter cell culture dish to reduce plastic waste. PLoS One 14:e0216251 (2019). PubMed: 31039189
- Shekaramiz E et al. Protein fishing from single live cells. J Nanobiotechnology 16:67 (2018). PubMed: 30205820
- Cagnetta R et al. Rapid Cue-Specific Remodeling of the Nascent Axonal Proteome. Neuron 99:29-46.e4 (2018). PubMed: 30008298
- Olthoff JT et al. Loss of peroxiredoxin-2 exacerbates eccentric contraction-induced force loss in dystrophin-deficient muscle. Nat Commun 9:5104 (2018). PubMed: 30504831