Anti-FITC 抗体 [F4/1] (ab112511)
Key features and details
- Mouse monoclonal [F4/1] to FITC
- Suitable for: IP, IHC-Fr, Flow Cyt
- Reacts with: Species independent
- Isotype: IgG1
製品の概要
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製品名
Anti-FITC antibody [F4/1]
FITC 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [F4/1] to FITC -
由来種
Mouse -
アプリケーション
適用あり: IP, IHC-Fr, Flow Cytmore details -
種交差性
交差種: Species independent -
免疫原
FITC conjugated to KLH
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
Preservative: 0.02% Sodium azide
Constituent: 99% PBS -
Concentration information loading...
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精製度
Protein G purified -
特記事項(精製)
Purified from tissue culture supernatant -
ポリ/モノ
モノクローナル -
クローン名
F4/1 -
ミエローマ
Sp2/0-Ag14 -
アイソタイプ
IgG1 -
軽鎖の種類
kappa -
研究分野
関連製品
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Compatible Secondaries
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Conjugation kits
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab112511の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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IP |
Use at an assay dependent concentration.
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IHC-Fr |
Use at an assay dependent concentration.
acetone-fixed |
|
Flow Cyt |
Use at an assay dependent concentration.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
特記事項 |
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IP
Use at an assay dependent concentration. |
IHC-Fr
Use at an assay dependent concentration. acetone-fixed |
Flow Cyt
Use at an assay dependent concentration. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
ターゲット情報
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関連性
FITC (fluorescein isothiocyanate) is a fluorochrome dye that absorbs ultraviolet or blue light causing molecules to become excited and emit a visible yellow-green light. This emission ceases upon removal of the light causing the excitation. Fluorochrome labeling provides rapid, accurate localization of antigen-antibody interaction when one of the reactants is part of a cell, tissue or other biological structure. FITC is a commonly used marker for antibodies in immunofluorescent techniques since the conjugation of FITC to proteins is relatively easy and does not, in general, destroy the biological activity of the labeled protein. FITC is widely used as a hapten to label different proteins. -
別名
- Fluorescein Isothiocyanate antibody
画像
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (3)
ab112511 は 3 報の論文で使用されています。
- Good Z et al. Proliferation tracing with single-cell mass cytometry optimizes generation of stem cell memory-like T cells. Nat Biotechnol 37:259-266 (2019). PubMed: 30742126
- Poeter M et al. Annexin A8 controls leukocyte recruitment to activated endothelial cells via cell surface delivery of CD63. Nat Commun 5:3738 (2014). PubMed: 24769558
- Balogh P et al. Hapten-mediated identification of cell membrane antigens using an anti-FITC monoclonal antibody. J Immunol Methods 169:35-40 (1994). PubMed: 7510754