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  1. Link

    fitc-antibody-ab19224.pdf

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Tags & Cell Markers Epitope Tags Conjugates
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Anti-FITC 抗体 (ab19224)

  • Datasheet
  • SDS
Submit a review Q&A (4)References (15)

Product price, shipping and contact information

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申し訳ございませんが、現在表示できません。

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出荷および受注について

Shipping info

Promotion Information

Abpromise

保証された製品品質、優れたカスタマー・サポート。 詳細はこちら。

Key features and details

  • Goat polyclonal to FITC
  • Suitable for: IP, ELISA, ICC, WB
  • Reacts with: Species independent
  • Isotype: IgG

こちらの製品もご検討ください

標識
Product image
FITC Conjugation Kit - Lightning-Link® (ab102884)
一次抗体
Anti-Digoxigenin antibody (ab76907)
二次抗体
Product image
Donkey Anti-Goat IgG H&L (HRP) (ab97110)

関連製品

製品の概要

  • 製品名

    Anti-FITC antibody
    FITC 一次抗体 製品一覧
  • 製品の詳細

    Goat polyclonal to FITC
  • 由来種

    Goat
  • アプリケーション

    適用あり: IP, ELISA, ICC, WBmore details
  • 種交差性

    交差種: Species independent
  • 免疫原

    Chemical/ Small Molecule corresponding to FITC conjugated to keyhole limpet haemocyanin.

  • 特記事項

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

  • 製品の状態

    Liquid
  • 保存方法

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファー

    pH: 6.8
    Preservative: 0.1% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • 精製度

    Immunogen affinity purified
  • ポリ/モノ

    ポリクローナル
  • アイソタイプ

    IgG
  • 研究分野

    • Tags & Cell Markers
    • Epitope Tags
    • Conjugates

関連製品

  • Compatible Secondaries

    • Donkey Anti-Goat IgG H&L (Alexa Fluor® 488) (ab150129)
    • Donkey Anti-Goat IgG H&L (HRP) (ab205723)
  • Isotype control

    • Goat IgG, polyclonal - Isotype Control (ab37373)

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab19224の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
IP
Use at an assay dependent concentration. PubMed: 17052454
ELISA
1/1000 - 1/30000. Suggested coating dilutions: 1/100 - 1/100.
ICC
1/200 - 1/2000.
WB
Use at an assay dependent concentration. Colorimetric detection: 1/1000 - 1/10000, Chemiluminescent detection: 1/1000 - 1/30000.
特記事項
IP
Use at an assay dependent concentration. PubMed: 17052454
ELISA
1/1000 - 1/30000. Suggested coating dilutions: 1/100 - 1/100.
ICC
1/200 - 1/2000.
WB
Use at an assay dependent concentration. Colorimetric detection: 1/1000 - 1/10000, Chemiluminescent detection: 1/1000 - 1/30000.

ターゲット情報

  • 関連性

    FITC (fluorescein isothiocyanate) is a fluorochrome dye that absorbs ultraviolet or blue light causing molecules to become excited and emit a visible yellow-green light. This emission ceases upon removal of the light causing the excitation. Fluorochrome labeling provides rapid, accurate localization of antigen-antibody interaction when one of the reactants is part of a cell, tissue or other biological structure. FITC is a commonly used marker for antibodies in immunofluorescent techniques since the conjugation of FITC to proteins is relatively easy and does not, in general, destroy the biological activity of the labeled protein. FITC is widely used as a hapten to label different proteins.
  • 別名

    • Fluorescein Isothiocyanate antibody

プロトコール

  • Immunocytochemistry & immunofluorescence protocols
  • Western blot protocols

Click here to view the general protocols

データシートおよび資料

  • SDS download

  • Datasheet download

    Download

参考文献 (15)

ab19224 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab19224 は 15 報の論文で使用されています。

  • Aftabizadeh M  et al. Potent antitumor effects of cell-penetrating peptides targeting STAT3 axis. JCI Insight 6:N/A (2021). PubMed: 33491667
  • Krishnamoorthy G  et al. FX11 limits Mycobacterium tuberculosis growth and potentiates bactericidal activity of isoniazid through host-directed activity. Dis Model Mech 13:N/A (2020). PubMed: 32034005
  • Azevedo C  et al. Development of a yeast model to study the contribution of vacuolar polyphosphate metabolism to lysine polyphosphorylation. J Biol Chem 295:1439-1451 (2020). PubMed: 31844018
  • Jeon SJ  et al. TIPRL potentiates survival of lung cancer by inducing autophagy through the eIF2a-ATF4 pathway. Cell Death Dis 10:959 (2019). PubMed: 31862913
  • Herrmann A  et al. An effective cell-penetrating antibody delivery platform. JCI Insight 4:N/A (2019). PubMed: 31341104
View all Publications for this product

レビューと Q&A

Show All レビュー Q&A
レビューを送る 質問を送る

1-4 of 4 Abreviews or Q&A

Question

Are these antibodies are exactly the same?

Read More

Abcam community

Verified customer

Asked on Feb 20 2013

Answer

Thank you for contacting us.

The antibodies are from different animals and are manufactured in different laboratories so we can expect some variation. The product ab19224 is against FITC which is a derivative of Flouroscein.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Read More

Abcam Scientific Support

Answered on Feb 20 2013

Question

Have you tried this product for IHC-Fr?

Read More

Abcam community

Verified customer

Asked on Jan 04 2012

Answer

Thank you very much for your interest in ab19224. To our knowledge, ab19224 has not been tested in IHC-Fr. Therefore, I can offer a discount off a future purchase if you buy ab19224 now, test it inIHC-Fr and submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of 1 free primary antibody. If you are interested in this offer, please follow these steps: 1. Reply to this e-mail to let me know that you would like to proceed and test ab19224 in IHC-Fr. I will then send a discount code. This code must be issued before purchasing ab19224 so please wait for my reply before ordering. 2. Purchase ab19224 either by phone, fax, or online (www.abcam.com). 3. Test it in IHC-Fr. 4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews. 5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for anyprimary antibodyordered and the discount code is valid for 4 months after issue. We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if ab19224 turns out to be unsuitable for IHC-Fr, you will still receive the discount on your next purchase after your Abreview has been submitted. Please let me know if you have any questions about this offer and I would be happy to help you further. The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.

Read More

Abcam Scientific Support

Answered on Jan 04 2012

Question

I would like to use this antibody to detect FITC conjugated to insulin in a rat skeletal muscle tissue sample. I'm rather confused, however, as to how this antibody recognizes FITC, which is not a protein but a molecule. How specific is it? Normally companies sell antibodies with representative examples of Western blots or applications they've used it for. I'm worried about purchasing this $300 antibody without knowing if there is evidence of it working or how it works.

Read More

Abcam community

Verified customer

Asked on Nov 15 2005

Answer

Thank you for your enquiry. Ab19224 reacts specifically with FITC and there is no reaction with KLH. The antibody has been characterized for application in Western blot, ELISA, and Immunocytochemistry. We don't have any images available at this time, but do guarantee that that this antibody will detect FITC in Western blotting, ELISA, and Immunocytochemistry. Our policy at Abcam is that if an antibody does not work as specified on the datasheet, we will offer a replacement or reimbursement. If you have any additional questions, please contact us again.

Read More

Abcam Scientific Support

Answered on Nov 16 2005

Question

Application: IHC - What tissues has this product been tested on?

Read More

Abcam community

Verified customer

Asked on Nov 01 2005

Answer

Thank you for your enquiry. It appears there was an error on the datasheet. This antibody has not been tested in IHC. I have updated the datasheet to reflect this, and I apologize for any inconvenience this may have caused. I hope this information helps. Please do not hesitate to contact us if you need anything further.

Read More

Abcam Scientific Support

Answered on Nov 02 2005

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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