FITC Anti-6X His tag® 抗体 (ab3554)

Reviews (1)Q&A (1)References (1)

Key features and details

  • FITC Chicken polyclonal to 6X His tag®
  • Suitable for: WB, ELISA
  • Reacts with: Species independent
  • Conjugation: FITC. Ex: 493nm, Em: 528nm
  • Isotype: IgY

製品の概要

  • 製品名

    FITC Anti-6X His tag® antibody
    6X His tag® 一次抗体 製品一覧

  • 製品の詳細

    FITC Chicken polyclonal to 6X His tag®

  • 由来種

    Chicken

  • 標識

    FITC. Ex: 493nm, Em: 528nm

  • アプリケーション

    適用あり: WB, ELISAmore details

  • 種交差性

    交差種: Species independent

  • 免疫原

    Synthetic peptide: HHHHHH conjugated to KLH.

  • 特記事項

    HIS-TAG® is a trademark of EMD Biosciences, Inc.

    Molar F/P ratio is 3.6:1.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C.

  • バッファー

    pH: 7.00
    Preservative: 0.1% Sodium azide
    Constituent: 0.2% BSA
  • Concentration information loading...

  • 精製度

    Immunogen affinity purified

  • ポリ/モノ

    ポリクローナル

  • ミエローマ

    unknown

  • アイソタイプ

    IgY

  • 軽鎖の種類

    unknown

  • 研究分野

関連製品

    アプリケーション

    The Abpromise guarantee

    Abpromise保証は、 次のテスト済みアプリケーションにおけるab3554の使用に適用されます

    アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

    アプリケーション Abreviews 特記事項
    WB
    Use at an assay dependent concentration.

    Prior to conjugation, the antibodies were analyzed by Western Blot.
    ELISA
    Use at an assay dependent concentration.

    Prior to conjugation, the antibodies were analyzed by ELISA.
    特記事項
    WB
    Use at an assay dependent concentration.

    Prior to conjugation, the antibodies were analyzed by Western Blot.
    ELISA
    Use at an assay dependent concentration.

    Prior to conjugation, the antibodies were analyzed by ELISA.

    ターゲット情報

    • 関連性

      The H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. The antibody recognizes the His-tag fused to the amino- or carboxy- termini of targeted proteins in transfected or transformed cells.

    • 細胞内局在

      Depends upon the localization of the parent protein tagged with hexahistidine.

    • 別名

      • 6 His epitope tag antibody
      • Hexa His tag antibody
      • HHHHHH epitope tag antibody
      • HHHHHH tag antibody
      • His tag antibody
      • Polyhistidine Tag antibody
      see all

    参考文献 (1)

    ab3554 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab3554 は 1 報の論文で使用されています。

    • Del Toro D  et al. Structural Basis of Teneurin-Latrophilin Interaction in Repulsive Guidance of Migrating Neurons. Cell 180:323-339.e19 (2020). PubMed: 31928845

    レビューと Q&A

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    1-2 of 2 Abreviews or Q&A

    Immunohistochemistry (Frozen sections) abreview for Anti-6X His tag® antibody (FITC)

     Excellent
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Blocking step
    Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 6% · Temperature: 21°C
    Sample
    Rat Tissue sections (spinal cord tissue)
    Specification
    spinal cord tissue
    Permeabilization
    Yes - TritonX
    Fixative
    Paraformaldehyde
    Read More

    Dr. Hayk Petrosyan

    Verified customer

    投稿 Jun 23 2014

    Question

    How do you see the westeren blot results with FITC conugated Ab? Thanks

    Read More
    Answer

    I cannot advise performing western blotting with a FITC antibody although it is likely to work given a suitable secondary antibody that recognizes the primary chicken antiserum. The reason behind the fact that western blotting is a listed application is that the antibody is derived from a parent antibody (the same as ab3553, an HRP conjugated 6X His tag antibody) and has been shown from the HRP form to work by western blotting. I would however recommend ab3554 for use by western blotting and ab3553 for western blotting.

    Read More

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