FITC Anti-6X His tag® 抗体 (ab1270)

Submit a review Q&A (2)References (1)

Key features and details

  • FITC Goat polyclonal to 6X His tag®
  • Suitable for: ICC
  • Reacts with: Species independent
  • Conjugation: FITC. Ex: 493nm, Em: 528nm
  • Isotype: IgG

製品の概要

  • 製品名

    FITC Anti-6X His tag® antibody
    6X His tag® 一次抗体 製品一覧

  • 製品の詳細

    FITC Goat polyclonal to 6X His tag®

  • 由来種

    Goat

  • 標識

    FITC. Ex: 493nm, Em: 528nm

  • アプリケーション

    適用あり: ICCmore details

  • 種交差性

    交差種: Species independent

  • 免疫原

    Synthetic peptide:

    HHHHHH

    (6-His) conjugated to KLH.
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • 特記事項

    HIS-TAG® is a trademark of EMD Biosciences, Inc.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C.

  • バッファー

    Preservative: 0.1% Sodium azide
    Constituent: 0.2% BSA
  • Concentration information loading...

  • 精製度

    Immunogen affinity purified

  • 特記事項（精製）

    Antibodies were affinity purified using the peptide immobilized on solid support. Antibody concentration was determined by extinction coefficient: absorbance at280 nm of 1.4 equals 1.0 mg of IgG.

  • ポリ/モノ

    ポリクローナル

  • アイソタイプ

    IgG

  • 研究分野

関連製品

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab1270の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
ICC
1/200 - 1/2000.
特記事項
ICC
1/200 - 1/2000.

ターゲット情報

  • 関連性

    The H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. The antibody recognizes the His-tag fused to the amino- or carboxy- termini of targeted proteins in transfected or transformed cells.

  • 細胞内局在

    Depends upon the localization of the parent protein tagged with hexahistidine.

  • 別名

    • 6 His epitope tag antibody
    • Hexa His tag antibody
    • HHHHHH epitope tag antibody
    • HHHHHH tag antibody
    • His tag antibody
    • Polyhistidine Tag antibody
    see all

参考文献 (1)

ab1270 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab1270 は 1 報の論文で使用されています。

  • Tai-Nagara I  et al. Placental labyrinth formation in mice requires endothelial FLRT2/UNC5B signaling. Development 144:2392-2401 (2017). PubMed: 28576770

レビューと Q&A

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1-2 of 2 Abreviews or Q&A

Question

Customer woul like to know: Does ab1270 react with the N terminus or C terminus end of 6-His?

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Answer

The antibody is designed to react with 6 His at the N-terminus and C-terminus.

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Question

I was wondering about the fixatives used for this antibody. Is paraformaldehyde ok, or must I use methanol, etc. More importantly, can this antibody be used on unfixed cells to detect the presence of a cell-surface HIS-tagged protein in unpermeabilized cells ?

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Answer

The method of fixation is unlikely to have significant effect on the His-tag. However, the fixation may influence the conformation of the tagged protein, and thereby, affect the accessibility of the His-tag. Thus, one must empirically determine the influence of fixation upon ability to detect tagged proteins.

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