FITC Anti-6X His tag® 抗体 (ab1270)

Submit a review Q&A (2)References (1)

Key features and details

  • FITC Goat polyclonal to 6X His tag®
  • Suitable for: ICC
  • Reacts with: Species independent
  • Conjugation: FITC. Ex: 493nm, Em: 528nm
  • Isotype: IgG

製品の概要

  • 製品名

    FITC Anti-6X His tag® antibody
    6X His tag® 一次抗体 製品一覧

  • 製品の詳細

    FITC Goat polyclonal to 6X His tag®

  • 由来種

    Goat

  • 標識

    FITC. Ex: 493nm, Em: 528nm

  • アプリケーション

    適用あり: ICCmore details

  • 種交差性

    交差種: Species independent

  • 免疫原

    Synthetic peptide:

    HHHHHH

    (6-His) conjugated to KLH.
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • 特記事項

    HIS-TAG® is a trademark of EMD Biosciences, Inc.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C.

  • バッファー

    Preservative: 0.1% Sodium azide
    Constituent: 0.2% BSA
  • Concentration information loading...

  • 精製度

    Immunogen affinity purified

  • 特記事項（精製）

    Antibodies were affinity purified using the peptide immobilized on solid support. Antibody concentration was determined by extinction coefficient: absorbance at280 nm of 1.4 equals 1.0 mg of IgG.

  • ポリ/モノ

    ポリクローナル

  • アイソタイプ

    IgG

  • 研究分野

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab1270 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC 1/200 - 1/2000.

ターゲット情報

  • 関連性

    The H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. The antibody recognizes the His-tag fused to the amino- or carboxy- termini of targeted proteins in transfected or transformed cells.

  • 細胞内局在

    Depends upon the localization of the parent protein tagged with hexahistidine.

  • 別名

    • 6 His epitope tag antibody
    • Hexa His tag antibody
    • HHHHHH epitope tag antibody
    • HHHHHH tag antibody
    • His tag antibody
    • Polyhistidine Tag antibody
    see all

参考文献 (1)

ab1270 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab1270 は 1 報の論文で使用されています。

  • Tai-Nagara I  et al. Placental labyrinth formation in mice requires endothelial FLRT2/UNC5B signaling. Development 144:2392-2401 (2017). PubMed: 28576770

レビューと Q&A

Show All レビュー Q&A
レビューを送る 質問を送る

1-2 of 2 Abreviews or Q&A

Question

Customer woul like to know: Does ab1270 react with the N terminus or C terminus end of 6-His?

Read More
Answer

The antibody is designed to react with 6 His at the N-terminus and C-terminus.

Read More

Question

I was wondering about the fixatives used for this antibody. Is paraformaldehyde ok, or must I use methanol, etc. More importantly, can this antibody be used on unfixed cells to detect the presence of a cell-surface HIS-tagged protein in unpermeabilized cells ?

Read More
Answer

The method of fixation is unlikely to have significant effect on the His-tag. However, the fixation may influence the conformation of the tagged protein, and thereby, affect the accessibility of the His-tag. Thus, one must empirically determine the influence of fixation upon ability to detect tagged proteins.

Read More

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com