Anti-FIP200 抗体 (ab176816)
Key features and details
- Rabbit polyclonal to FIP200
- Suitable for: IP, WB
- Reacts with: Mouse, Human
- Isotype: IgG
製品の概要
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製品名
Anti-FIP200 antibody
FIP200 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to FIP200 -
由来種
Rabbit -
アプリケーション
適用あり: IP, WBmore details -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Rat, Horse, Guinea pig, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Common marmoset, Orangutan -
免疫原
Synthetic peptide within Human FIP200 aa 1544-1594 (C terminal). The exact sequence is proprietary. NP_055596.3
Sequence:EGASGAS RRPWVLGKVM EKEYCQAKKA QNRFKVPLGT KFYRVKAVSW NKKV
Database link: Q8TDY2 -
ポジティブ・コントロール
- HeLa, 293T and TCMK1 whole cell lysates.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 99% Tris buffered saline, 0.1% BSA -
Concentration information loading...
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精製度
Immunogen affinity purified -
特記事項(精製)
ab176816 was affinity purified using an epitope specific to FIP200 immobilized on solid support. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab176816の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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IP |
Use at 2-5 µg/mg of lysate.
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WB |
1/2000 - 1/10000. Predicted molecular weight: 183 kDa.
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特記事項 |
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IP
Use at 2-5 µg/mg of lysate. |
WB
1/2000 - 1/10000. Predicted molecular weight: 183 kDa. |
ターゲット情報
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機能
Implicated in the regulation of RB1 expression. Functions as a DNA-binding transcription factor. Is a potent regulator of the RB1 pathway and a mediator that plays a crucial role in muscular differentiation. Expression is, thus, a prerequisite for myogenic differentiation. Involved in autophagy. Required for autophagosome formation (By similarity). Inhibits PTK2/FAK1 and PTK2B/PYK2 activity and activation of downstream signaling pathways. -
組織特異性
Expression levels correlated closely with those of RB1 in cancer cell lines as well as in various normal human tissues. Abundantly expressed in human musculoskeletal and cultured osteosarcoma cells. -
発生段階
Expression was difficult to detect in immature proliferating chondroblasts or myogenic cells in embryos, but became obvious and prominent concomitantly with the maturation of osteocytes, chondrocytes, and skeletal muscle cells. Expression in these musculoskeletal cells increased with RB1 expression, which is linked to the terminal differentiation of many tissues and cells. The introduction of the wild-type protein decreased the formation of macroscopic colonies in a cell growth assay. -
細胞内局在
Nucleus. Cytoplasm > cytosol. Preautophagosomal structure. Under starvation conditions, is localized to puncate structures primarily representing the isolation membrane that sequesters a portion of the cytoplasm resulting in the formation of an autophagosome. - Information by UniProt
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参照データベース
- Entrez Gene: 464173 Chimpanzee
- Entrez Gene: 100407986 Common marmoset
- Entrez Gene: 539858 Cow
- Entrez Gene: 477876 Dog
- Entrez Gene: 100717631 Guinea pig
- Entrez Gene: 100050327 Horse
- Entrez Gene: 9821 Human
- Entrez Gene: 12421 Mouse
see all -
別名
- FAK family kinase-interacting protein of 200 kDa antibody
- FIP200 antibody
- RB1-inducible coiled-coil protein 1 antibody
see all
画像
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All lanes : Anti-FIP200 antibody (ab176816) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysates at 50 µg
Lane 2 : HeLa whole cell lysates at 15 µg
Lane 3 : HeLa whole cell lysates at 5 µg
Lane 4 : 293T whole cell lysates at 50 µg
Developed using the ECL technique.
Predicted band size: 183 kDa
Exposure time: 30 seconds -
All lanes : Anti-FIP200 antibody (ab176816) at 0.2 µg/ml
Lane 1 : TCMK1 whole cell lysates at 50 µg
Lane 2 : TCMK1 whole cell lysates at 15 µg
Developed using the ECL technique.
Predicted band size: 183 kDa
Exposure time: 3 minutes -
ab176816 at 0.1 µg/ml detecting FIP200 in HeLa whole cell lysate by WB following IP.
Lane 1: ab176816 at 3µg/mg of lysate
Lane 2: Control IgG.In each case, 1 mg of lysate was used for IP and 20% of the IP was loaded.
Detection: Chemiluminescence with an exposure time of 30 seconds.
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (6)
ab176816 は 6 報の論文で使用されています。
- Zhu W et al. Silencing of miR-497-5p inhibits cell apoptosis and promotes autophagy in Parkinson's disease by upregulation of FGF2. Environ Toxicol 36:2302-2312 (2021). PubMed: 34459097
- Xu HJ et al. Down-regulation of exosomal microRNA-224-3p derived from bone marrow-derived mesenchymal stem cells potentiates angiogenesis in traumatic osteonecrosis of the femoral head. FASEB J N/A:fj201801618RRR (2019). PubMed: 30964699
- Dudley LJ et al. Intrinsic lipid binding activity of ATG16L1 supports efficient membrane anchoring and autophagy. EMBO J 38:N/A (2019). PubMed: 30936093
- Fraser J et al. Targeting of early endosomes by autophagy facilitates EGFR recycling and signalling. EMBO Rep 20:e47734 (2019). PubMed: 31448519
- Shin JH et al. Down-regulated TMED10 in Alzheimer disease induces autophagy via ATG4B activation. Autophagy 15:1495-1505 (2019). PubMed: 30821607
- Fang W et al. miR-224-3p inhibits autophagy in cervical cancer cells by targeting FIP200. Sci Rep 6:33229 (2016). PubMed: 27615604