Anti-Filaggrin 抗体 [FLG/1563] - BSA and Azide free (ab237839)
Key features and details
- Mouse monoclonal [FLG/1563] to Filaggrin - BSA and Azide free
- Suitable for: Protein Array, IHC-P
- Reacts with: Human
- Isotype: IgG2b
Related conjugates and formulations
製品の概要
-
製品名
Anti-Filaggrin antibody [FLG/1563] - BSA and Azide free
Filaggrin 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [FLG/1563] to Filaggrin - BSA and Azide free -
由来種
Mouse -
アプリケーション
適用あり: Protein Array, IHC-Pmore details -
種交差性
交差種: Human -
免疫原
Recombinant fragment within Human Filaggrin aa 998-1104. The exact sequence is proprietary.
Database link: P20930 -
ポジティブ・コントロール
- IHC-P: Human skin tissue.
-
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.2
Constituent: PBS -
キャリア・フリー
はい -
Concentration information loading...
-
精製度
Protein A/G purified -
特記事項(精製)
Purified from bioreactor concentrate by Protein A/G. -
ポリ/モノ
モノクローナル -
クローン名
FLG/1563 -
アイソタイプ
IgG2b -
軽鎖の種類
kappa -
研究分野
関連製品
-
Alternative Versions
-
Compatible Secondaries
-
Conjugation kits
-
Isotype control
-
Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab237839の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
Protein Array |
Use at an assay dependent concentration.
|
|
IHC-P |
Use a concentration of 0.5 - 1 µg/ml.
Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at room temperaturefor 20 minutes. Incubate with primary antibody for 30 minutes at room temperature. |
特記事項 |
---|
Protein Array
Use at an assay dependent concentration. |
IHC-P
Use a concentration of 0.5 - 1 µg/ml. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at room temperaturefor 20 minutes. Incubate with primary antibody for 30 minutes at room temperature. |
ターゲット情報
-
機能
Aggregates keratin intermediate filaments and promotes disulfide-bond formation among the intermediate filaments during terminal differentiation of mammalian epidermis. -
組織特異性
Keratohyalin granules. -
関連疾患
Ichthyosis vulgaris
Dermatitis atopic 2 -
配列類似性
Belongs to the S100-fused protein family.
Contains 2 EF-hand domains.
Contains 23 filaggrin repeats. -
翻訳後修飾
Filaggrin is initially synthesized as a large, insoluble, highly phosphorylated precursor containing many tandem copies of 324 AA, which are not separated by large linker sequences. During terminal differentiation it is dephosphorylated and proteolytically cleaved. The N-terminal of the mature protein is heterogeneous, and is blocked by the formation of pyroglutamate.
Undergoes deimination of some arginine residues (citrullination). - Information by UniProt
-
参照データベース
- Entrez Gene: 2312 Human
- Omim: 135940 Human
- SwissProt: P20930 Human
- Unigene: 654510 Human
-
別名
- ATOD2 antibody
- Epidermal filaggrin antibody
- FILA_HUMAN antibody
see all
画像
-
This data was produced with ab237996, the same antibody in a different formulation with BSA and Azide.
ab237996 was tested in protein array against over 19000 different full-length human proteins.
Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target.
A MAb is specific to its intended target if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29. -
Formalin-fixed, paraffin-embedded human skin tissue stained for Filaggrin with ab237839 at 1 µg/ml in immunohistochemical analysis.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
-
Datasheet download
参考文献 (0)
ab237839 は論文での使用が確認できていません。