Anti-FIF 抗体 (ab99307)
Key features and details
- Rabbit polyclonal to FIF
- Suitable for: WB, IP, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
製品の概要
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製品名
Anti-FIF antibody
FIF 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to FIF -
由来種
Rabbit -
アプリケーション
適用あり: WB, IP, IHC-Pmore details -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Rat, Rabbit, Horse, Chicken, Guinea pig, Pig, Xenopus laevis, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Xenopus tropicalis, Platypus
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免疫原
Synthetic peptide corresponding to Human FIF aa 1-50.
Database link: XP_508375.2 -
ポジティブ･コントロール
- Whole cell lysates from HeLa, 293T and Mouse NIH 3T3 cells.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab99307の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/2000 - 1/10000. Predicted molecular weight: 58 kDa.
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| IP |
Use at 2-5 µg/mg of lysate.
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| IHC-P |
1/100 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| 特記事項 |
|---|
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WB
1/2000 - 1/10000. Predicted molecular weight: 58 kDa. |
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IP
Use at 2-5 µg/mg of lysate. |
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IHC-P
1/100 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ターゲット情報
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機能
Antiapoptotic factor that may have a role in protein assembly. Negatively regulates ACIN1. By binding to ACIN1, it suppresses ACIN1 cleavage from CASP3 and ACIN1-mediated DNA fragmentation. Also known to efficiently suppress E2F1-induced apoptosis. Its depletion enhances the cytotoxic action of the chemotherapeutic drugs. -
組織特異性
Expressed in all tissues tested, including heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. Highest levels in heart, pancreas and placenta. Highly expressed in several cancers. Preferentially expressed in squamous cell carcinoma versus adenocarcinoma in non-small cell lung cancer. -
配列類似性
Belongs to the API5 family. -
ドメイン
Two regions, an N-terminal (aa 96-107) and a C-terminal (aa 274-311) are required for binding FGF2. -
細胞内局在
Cytoplasm and Nucleus. Cytoplasm. Mainly nuclear. Can also be cytoplasmic. - Information by UniProt
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参照データベース
- Entrez Gene: 8539 Human
- Entrez Gene: 11800 Mouse
- Omim: 609774 Human
- SwissProt: Q9BZZ5 Human
- SwissProt: O35841 Mouse
- Unigene: 435771 Human
- Unigene: 181824 Mouse
- Unigene: 402557 Mouse
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別名
- AAC 11 antibody
- AAC-11 antibody
- AAC11 antibody
see all
画像
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Western blot - Anti-FIF antibody (ab99307)All lanes : Anti-FIF antibody (ab99307) at 0.04 µg/ml
Lane 1 : HeLa whole cell
lysate at 50 µg
Lane 2 : HeLa whole cell
lysate at 15 µg
Lane 3 : HeLa whole cell
lysate at 5 µg
Lane 4 : 293T whole cell
lysate at 50 µg
Lane 5 : NIH 3T3 whole cell
lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 58 kDa
Exposure time: 10 seconds -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FIF antibody (ab99307)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma (left) and mouse teratoma (right) tissues labelling FIF with ab99307 at 1/1000 (0.2µg/mg) and 1/200 (1 µg/mg). Detection: DAB.
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Immunoprecipitation - Anti-FIF antibody (ab99307)ab99307, at 0.4 µg/ml, staining FIF in HeLa whole cell lysate immunoprecipitated using ab99307 at 3 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection: Chemiluminescence with an exposure time of 1 second.
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (0)
ab99307 は論文での使用が確認できていません。
