Anti-FIF 抗体 (ab99307)
製品の概要
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製品名Anti-FIF antibody
FIF 一次抗体 製品一覧 -
製品の詳細Rabbit polyclonal to FIF
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由来種Rabbit
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アプリケーション適用あり: WB, IP, IHC-Pmore details
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種交差性交差種: Mouse, Human
交差が予測される動物種: Rat, Rabbit, Horse, Chicken, Guinea pig, Pig, Xenopus laevis, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Xenopus tropicalis, Platypus
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免疫原
Synthetic peptide corresponding to Human FIF aa 1-50.
Database link: XP_508375.2 -
ポジティブ･コントロール
- Whole cell lysates from HeLa, 293T and Mouse NIH 3T3 cells.
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特記事項
Previously labelled as Apoptosis inhibitor 5.
製品の特性
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製品の状態Liquid
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保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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バッファーPreservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading... -
精製度Immunogen affinity purified
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ポリ/モノポリクローナル
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アイソタイプIgG
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研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
アプリケーション
Our Abpromise guarantee covers the use of ab99307 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | 1/2000 - 1/10000. Predicted molecular weight: 58 kDa. | |
| IP | Use at 2-5 µg/mg of lysate. | |
| IHC-P | 1/100 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ターゲット情報
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機能Antiapoptotic factor that may have a role in protein assembly. Negatively regulates ACIN1. By binding to ACIN1, it suppresses ACIN1 cleavage from CASP3 and ACIN1-mediated DNA fragmentation. Also known to efficiently suppress E2F1-induced apoptosis. Its depletion enhances the cytotoxic action of the chemotherapeutic drugs.
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組織特異性Expressed in all tissues tested, including heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. Highest levels in heart, pancreas and placenta. Highly expressed in several cancers. Preferentially expressed in squamous cell carcinoma versus adenocarcinoma in non-small cell lung cancer.
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配列類似性Belongs to the API5 family.
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ドメインTwo regions, an N-terminal (aa 96-107) and a C-terminal (aa 274-311) are required for binding FGF2.
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細胞内局在Cytoplasm and Nucleus. Cytoplasm. Mainly nuclear. Can also be cytoplasmic.
- Information by UniProt
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参照データベース
- Entrez Gene: 8539 Human
- Entrez Gene: 11800 Mouse
- Omim: 609774 Human
- SwissProt: Q9BZZ5 Human
- SwissProt: O35841 Mouse
- Unigene: 435771 Human
- Unigene: 181824 Mouse
- Unigene: 402557 Mouse
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別名
- AAC 11 antibody
- AAC-11 antibody
- AAC11 antibody
see all
画像
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Western blot - Anti-FIF antibody (ab99307)All lanes : Anti-FIF antibody (ab99307) at 0.04 µg/ml
Lane 1 : HeLa whole cell
lysate at 50 µg
Lane 2 : HeLa whole cell
lysate at 15 µg
Lane 3 : HeLa whole cell
lysate at 5 µg
Lane 4 : 293T whole cell
lysate at 50 µg
Lane 5 : NIH 3T3 whole cell
lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 58 kDa
Exposure time: 10 seconds -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FIF antibody (ab99307)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma (left) and mouse teratoma (right) tissues labelling FIF with ab99307 at 1/1000 (0.2µg/mg) and 1/200 (1 µg/mg). Detection: DAB.
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Immunoprecipitation - Anti-FIF antibody (ab99307)ab99307, at 0.4 µg/ml, staining FIF in HeLa whole cell lysate immunoprecipitated using ab99307 at 3 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection: Chemiluminescence with an exposure time of 1 second.
データシートおよび資料
参考文献
ab99307 has not yet been referenced specifically in any publications.
