Anti-Fibulin-4 抗体 [EPR684(2)] - BSA and Azide free (ab248111)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR684(2)] to Fibulin-4 - BSA and Azide free
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Human
Related conjugates and formulations
製品の概要
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製品名
Anti-Fibulin-4 antibody [EPR684(2)] - BSA and Azide free
Fibulin-4 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR684(2)] to Fibulin-4 - BSA and Azide free -
由来種
Rabbit -
アプリケーション
適用あり: IHC-P, WBmore details
適用なし: Flow Cyt,ICC/IF or IP -
種交差性
交差種: Mouse, Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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特記事項
ab248111 is the carrier-free version of ab125073.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
バッファー
pH: 7.2
Constituent: PBS -
キャリア・フリー
はい -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR684(2) -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Conjugation kits
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab248111の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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IHC-P |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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WB |
Use at an assay dependent concentration. Predicted molecular weight: 49 kDa.
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特記事項 |
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IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
Use at an assay dependent concentration. Predicted molecular weight: 49 kDa. |
ターゲット情報
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関連疾患
Defects in EFEMP2 are a cause of cutis laxa autosomal recessive type 1 (ARCL1) [MIM:219100]. Hereditary cutis laxa refers to a heterogeneous group of connective tissue disorders characterized by cutaneous abnormalities and variable systemic manifestations. The most constant clinical feature is loose skin, sagging over the face and trunk. Hereditary cutis laxa is inherited in both autosomal dominant and autosomal recessive modes. ARCL1 shows the most severe phenotype and has the poorest prognosis. In addition to the skin, internal organs enriched in elastic fibers, such as the lung and arteries, are affected. -
配列類似性
Belongs to the fibulin family.
Contains 6 EGF-like domains. -
細胞内局在
Secreted. - Information by UniProt
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参照データベース
- Entrez Gene: 30008 Human
- Entrez Gene: 58859 Mouse
- Omim: 604633 Human
- SwissProt: O95967 Human
- SwissProt: Q9WVJ9 Mouse
- Unigene: 170622 Human
- Unigene: 276367 Mouse
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別名
- EFEMP 2 antibody
- EFEMP2 antibody
- EGF containing fibulin like extracellular matrix protein 2 antibody
see all
画像
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All lanes : Anti-Fibulin-4 antibody [EPR684(2)] (ab125073) at 1/1000 dilution
Lane 1 : HEK-293T (human embryonic kidney) whole cell lysates
Lane 2 : HepG2 (human hepatocellular carcinoma) whole cell lysates
Lane 3 : Human fetal lung tissue lysates
Lane 4 : Human colon cancer tissue lysates
Lane 5 : Human placenta tissue lysates
Lane 6 : Mouse placenta tissue lysates
Lane 7 : Mouse lung tissue lysates
Lane 8 : NIH/3T3 (mouse embryo) whole cell lysates
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 49 kDa
Observed band size: 55 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutesThis data was developed using ab125073, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
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All lanes : Anti-Fibulin-4 antibody [EPR684(2)] (ab125073) at 1/1000 dilution
Lane 1 : JAR cell lysate
Lane 2 : SW480 cell lysate
Lane 3 : Human fetal lung tissue lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 49 kDaThis data was developed using ab125073, the same antibody clone in a different buffer formulation.
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This data was developed using ab125073, the same antibody clone in a different buffer formulation.ab125073, at 1/250, staining Fibulin-4 in paraffin-embedded Human breast carcinoma tissue by immunohistochemistry. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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This data was developed using ab125073, the same antibody clone in a different buffer formulation.ab125073, at 1/250, staining Fibulin-4 in paraffin-embedded Human muscle tissue by immunohistochemistry. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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Datasheet download
Certificate of Compliance
参考文献 (0)
ab248111 は論文での使用が確認できていません。