Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (bone and bone marrow)
Antigen retrieval step
None
Permeabilization
Yes - 20%DMSO
Specification
bone and bone marrow
Blocking step
Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Fixative
Paraformaldehyde
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 21°C · Diluent: Blocking Buffer
Dilution
1/100
Secondary antibody
Name
Non-Abcam antibody was used: anti-rabbit IgG
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 555
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 555
Additional data
Additional Notes
Femur sections with 250um thickness.
Secondary anti-rabbit conjugate Alexa Fluor 555 stains the anti-fibronectin (red).
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
投稿 Sep 30 2019