Anti-Fibronectin 抗体 (ab2413)
Key features and details
- Rabbit polyclonal to Fibronectin
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-Fibronectin antibody
Fibronectin 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Fibronectin -
由来種
Rabbit -
アプリケーション
適用あり: ICC/IF, WB, IHC-Pmore details -
種交差性
交差種: Mouse, Human -
免疫原
Other Immunogen Type corresponding to Human Fibronectin.
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ポジティブ･コントロール
- WB: Human colon tissue lysate, HepG2, NIH 3T3 whole cell lysate. IHC-P: Human kidney tissue. ICC: HeLa cells.
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特記事項
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. -
バッファー
Preservative: 0.05% Sodium azide -
Concentration information loading... -
精製度
Affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
軽鎖の種類
unknown -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
アプリケーション
Our Abpromise guarantee covers the use of ab2413 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ICC/IF | Use at an assay dependent concentration. | |
| WB | Use at an assay dependent concentration. Detects a band of approximately 285 kDa (predicted molecular weight: 262 kDa). | |
| IHC-P | Use at an assay dependent concentration. |
ターゲット情報
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機能
Fibronectins bind cell surfaces and various compounds including collagen, fibrin, heparin, DNA, and actin. Fibronectins are involved in cell adhesion, cell motility, opsonization, wound healing, and maintenance of cell shape. Involved in osteoblast compaction through the fibronectin fibrillogenesis cell-mediated matrix assembly process, essential for osteoblast mineralization. Participates in the regulation of type I collagen deposition by osteoblasts.
Anastellin binds fibronectin and induces fibril formation. This fibronectin polymer, named superfibronectin, exhibits enhanced adhesive properties. Both anastellin and superfibronectin inhibit tumor growth, angiogenesis and metastasis. Anastellin activates p38 MAPK and inhibits lysophospholipid signaling. -
組織特異性
Plasma FN (soluble dimeric form) is secreted by hepatocytes. Cellular FN (dimeric or cross-linked multimeric forms), made by fibroblasts, epithelial and other cell types, is deposited as fibrils in the extracellular matrix. Ugl-Y1, Ugl-Y2 and Ugl-Y3 are found in urine. -
関連疾患
Glomerulopathy with fibronectin deposits 2 -
配列類似性
Contains 12 fibronectin type-I domains.
Contains 2 fibronectin type-II domains.
Contains 16 fibronectin type-III domains. -
発生段階
Ugl-Y1, Ugl-Y2 and Ugl-Y3 are present in the urine from 0 to 17 years of age. -
翻訳後修飾
Sulfated.
It is not known whether both or only one of Thr-2064 and Thr-2065 are/is glycosylated.
Forms covalent cross-links mediated by a transglutaminase, such as F13A or TGM2, between a glutamine and the epsilon-amino group of a lysine residue, forming homopolymers and heteropolymers (e.g. fibrinogen-fibronectin, collagen-fibronectin heteropolymers).
Phosphorylated by FAM20C in the extracellular medium.
Proteolytic processing produces the C-terminal NC1 peptide, anastellin. -
細胞内局在
Secreted, extracellular space, extracellular matrix. - Information by UniProt
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参照データベース
- Entrez Gene: 2335 Human
- Entrez Gene: 14268 Mouse
- Omim: 135600 Human
- SwissProt: P02751 Human
- SwissProt: P11276 Mouse
- Unigene: 203717 Human
- Unigene: 193099 Mouse
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別名
- CIG antibody
- Cold insoluble globulin antibody
- Cold-insoluble globulin antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibronectin antibody (ab2413)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling Fibronectin with ab2413 at 1/50 dilution. Antigen retrieval performed with EDTA buffer pH 8 before commencing with IHC staining protocol.
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Western blot - Anti-Fibronectin antibody (ab2413)All lanes : Anti-Fibronectin antibody (ab2413) at 1 µg/ml
Lane 1 : Human colon tissue lysate
Lane 2 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lane 3 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Predicted band size: 262 kDa
Observed band size: 285 kDa why is the actual band size different from the predicted? -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibronectin antibody (ab2413)Immunohistochemical analysis of formalin-fixed, paraffin-embedded human kidney tissue, staining Fibronectin with ab2413.
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Immunocytochemistry/ Immunofluorescence - Anti-Fibronectin antibody (ab2413)ICC/IF image of ab2413 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab2413, 1 µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue). This antibody also gave a positive IF result in Hek293, HepG2 and MCF7 cells. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibronectin antibody (ab2413)ab2413 staining Fibronectin in human kidney tissue section by IHC-P (Formalin/PFA-fixed paraffin embedded tissue sections). Tissue sections were incubated with ab2413 at a dilution of 1:250 for one hour. Heat mediated antigen retrieval technique was used with citrate buffer at pH 6.0. DAB staining was done with a biotinylated secondary for 45 min at RT at a concentration of 1:1000.
プロトコール
参考文献 (422)
ab2413 は 422 報の論文で使用されています。
- Qin T et al. Prediction of the mechanisms of action of Shenkang in chronic kidney disease: A network pharmacology study and experimental validation. J Ethnopharmacol 246:112128 (2020). PubMed: 31386888
- Zhou S et al. Endoplasmic reticulum stress regulates epithelial-mesenchymal transition in human lens epithelial cells. Mol Med Rep 21:173-180 (2020). PubMed: 31746423
- Gao L et al. TNAP inhibition attenuates cardiac fibrosis induced by myocardial infarction through deactivating TGF-ß1/Smads and activating P53 signaling pathways. Cell Death Dis 11:44 (2020). PubMed: 31969558
- Pap D et al. Characterization of IL-19, -20, and -24 in acute and chronic kidney diseases reveals a pro-fibrotic role of IL-24. J Transl Med 18:172 (2020). PubMed: 32306980
- Nielsen PM et al. Hyperpolarized [1-13 C] alanine production: A novel imaging biomarker of renal fibrosis. Magn Reson Med 84:2063-2073 (2020). PubMed: 32452096
レビューと Q&A
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