Anti-Fibromodulin 抗体 [EPR23029-174] - BSA and Azide free (ab269866)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR23029-174] to Fibromodulin - BSA and Azide free
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
製品の概要
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製品名
Anti-Fibromodulin antibody [EPR23029-174] - BSA and Azide free
Fibromodulin 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR23029-174] to Fibromodulin - BSA and Azide free -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details
適用なし: IHC-P or IP -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- WB: Recombinant Human Fibromodulin/FMOD protein, Human articular cartilage, Human uterus, Mouse articular cartilage, Rat articular cartilage, Human uterus and Human uterus treated with PNGase F lysates.
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特記事項
ab269866 is the carrier-free version of ab267465.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
バッファー
pH: 7.2
Constituent: PBS -
キャリア・フリー
はい -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR23029-174 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab269866の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
Use at an assay dependent concentration. Predicted molecular weight: 43 kDa.
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特記事項 |
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WB
Use at an assay dependent concentration. Predicted molecular weight: 43 kDa. |
ターゲット情報
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機能
Affects the rate of fibrils formation. May have a primary role in collagen fibrillogenesis. -
配列類似性
Belongs to the small leucine-rich proteoglycan (SLRP) family. SLRP class II subfamily.
Contains 11 LRR (leucine-rich) repeats.
Contains 1 LRRNT domain. -
翻訳後修飾
Binds keratan sulfate chains. -
細胞内局在
Secreted > extracellular space > extracellular matrix. - Information by UniProt
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参照データベース
- Entrez Gene: 2331 Human
- Entrez Gene: 14264 Mouse
- Entrez Gene: 64507 Rat
- Omim: 600245 Human
- SwissProt: Q06828 Human
- SwissProt: P50608 Mouse
- SwissProt: P50609 Rat
- Unigene: 519168 Human
see all -
別名
- Collagen binding 59 kDa protein antibody
- Collagen-binding 59 kDa protein antibody
- Fibromodulin antibody
see all
画像
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All lanes : Anti-Fibromodulin antibody [EPR23029-174] (ab267465) at 1/1000 dilution
Lane 1 : Human articular cartilage lysate
Lane 2 : Human uterus lysate
Lane 3 : Human skin lysate
Lane 4 : HuT-78 (human sezary syndrome cutaneous t lymphocyte) whole cell lysate
Lane 5 : Mouse articular cartilage lysate
Lane 6 : Rat articular cartilage lysate
Lysates/proteins at 20 µg per lane.
Secondary
Lanes 1-4 : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution
Lanes 5-6 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/1000 dilution
Predicted band size: 43 kDaBlocking and diluting buffer and concentration: 5% NFDM/TBST
The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 12623288, 8895216)
Negative control: Hut-78 (PMID: 15741214)
Exposure time: Lane 1 second; Lanes 2-6:3 secondsThis data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab267465).
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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Datasheet download
Certificate of Compliance
参考文献 (0)
ab269866 は論文での使用が確認できていません。