リコンビナントRabMAb

Anti-Fibromodulin 抗体 [EPR23029-174] - BSA and Azide free (ab269866)

Submit a review Submit a question
Western blot - Anti-Fibromodulin antibody [EPR23029-174] - BSA and Azide free (ab269866)
  • Anti-Fibromodulin antibody [EPR23029-174] - BSA and Azide free (ab269866)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23029-174] to Fibromodulin - BSA and Azide free
  • Suitable for: WB
  • Reacts with: Mouse, Rat, Human

製品の概要

  • 製品名

    Anti-Fibromodulin antibody [EPR23029-174] - BSA and Azide free
    Fibromodulin 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR23029-174] to Fibromodulin - BSA and Azide free

  • 由来種

    Rabbit

  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data

  • 免疫原

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • WB: Recombinant Human Fibromodulin/FMOD protein, Human articular cartilage, Human uterus, Mouse articular cartilage, Rat articular cartilage, Human uterus and Human uterus treated with PNGase F lysates.

  • 特記事項

    ab269866 is the carrier-free version of ab267465. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

アプリケーション

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab269866 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

アプリケーション Species
WB
Human
アプリケーション Abreviews 特記事項
WB
Use at an assay dependent concentration. Predicted molecular weight: 43 kDa.
特記事項
WB
Use at an assay dependent concentration. Predicted molecular weight: 43 kDa.
  • 追加情報
    Is unsuitable for IHC-P or IP.

    • ターゲット情報

    • 機能

      Affects the rate of fibrils formation. May have a primary role in collagen fibrillogenesis.

    • 配列類似性

      Belongs to the small leucine-rich proteoglycan (SLRP) family. SLRP class II subfamily.
      Contains 11 LRR (leucine-rich) repeats.
      Contains 1 LRRNT domain.

    • 翻訳後修飾

      Binds keratan sulfate chains.

    • 細胞内局在

      Secreted > extracellular space > extracellular matrix.
    • Information by UniProt

    • 参照データベース

      see all

    • 別名

      • Collagen binding 59 kDa protein antibody
      • Collagen-binding 59 kDa protein antibody
      • Fibromodulin antibody
      • Fibromodulin precursor antibody
      • Fibromodulin proteoglycan antibody
      • FM antibody
      • FMOD antibody
      • FMOD_HUMAN antibody
      • Keratan sulfate proteoglycan fibromodulin antibody
      • KSPG fibromodulin antibody
      • SLRR2E antibody
      see all

    画像

    • Western blot - Anti-Fibromodulin antibody [EPR23029-174] - BSA and Azide free (ab269866)
      Western blot - Anti-Fibromodulin antibody [EPR23029-174] - BSA and Azide free (ab269866)
      All lanes : Anti-Fibromodulin antibody [EPR23029-174] (ab267465) at 1/1000 dilution

      Lane 1 : Human articular cartilage lysate
      Lane 2 : Human uterus lysate
      Lane 3 : Human skin lysate
      Lane 4 : HuT-78 (human sezary syndrome cutaneous t lymphocyte) whole cell lysate
      Lane 5 : Mouse articular cartilage lysate
      Lane 6 : Rat articular cartilage lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      Lanes 1-4 : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution
      Lanes 5-6 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/1000 dilution

      Predicted band size: 43 kDa



      Blocking and diluting buffer and concentration: 5% NFDM/TBST

      The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 12623288, 8895216) 

      Negative control: Hut-78 (PMID: 15741214)

      Exposure time: Lane 1 second; Lanes 2-6:3 secondsThis data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab267465).

    • Anti-Fibromodulin antibody [EPR23029-174] - BSA and Azide free (ab269866)
      Anti-Fibromodulin antibody [EPR23029-174] - BSA and Azide free (ab269866)

    プロトコール

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    参考文献 (0)

    ab269866 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab269866 は論文での使用が確認できていません。

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    There are currently no Customer reviews or Questions for ab269866.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com