Anti-Fibrinogen alpha chain 抗体 [UC45] (ab19079)

Reviews (2)Q&A (2)References (8)

Key features and details

  • Mouse monoclonal [UC45] to Fibrinogen alpha chain
  • Suitable for: Flow Cyt
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgM

製品の概要

  • 製品名

    Anti-Fibrinogen alpha chain antibody [UC45]
    Fibrinogen alpha chain 一次抗体 製品一覧

  • 製品の詳細

    Mouse monoclonal [UC45] to Fibrinogen alpha chain

  • 由来種

    Mouse

  • 特異性

    This antibody reacts very strongly with the alpha chain of human fibrinogen, for further information please see Hogg 1983 paper quoted.

  • アプリケーション

    適用あり: Flow Cytmore details

  • 種交差性

    交差種: Mouse, Rat, Human

  • 免疫原

    Human acute monoblastic leukemia cells.

  • 特記事項


    A monocyte and neuronal antigen present predominantly on a protein of 45kDa MW. UC45 is specific for monocytic leukemias. The relevant antigens are not expressed in all cases with monocytic differentiation.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab19079 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Flow Cyt Use 1µg for 106 cells.

ab91545 - Mouse monoclonal IgM, is suitable for use as an isotype control with this antibody.

 

ターゲット情報

  • 機能

    Fibrinogen has a double function: yielding monomers that polymerize into fibrin and acting as a cofactor in platelet aggregation.

  • 組織特異性

    Plasma.

  • 関連疾患

    Defects in FGA are a cause of congenital afibrinogenemia (CAFBN) [MIM:202400]. This is a rare autosomal recessive disorder characterized by bleeding that varies from mild to severe and by complete absence or extremely low levels of plasma and platelet fibrinogen. Note=The majority of cases of afibrinogenemia are due to truncating mutations. Variations in position Arg-35 (the site of cleavage of fibrinopeptide a by thrombin) leads to alpha-dysfibrinogenemias.
    Defects in FGA are a cause of amyloidosis type 8 (AMYL8) [MIM:105200]; also known as systemic non-neuropathic amyloidosis or Ostertag-type amyloidosis. AMYL8 is a hereditary generalized amyloidosis due to deposition of apolipoprotein A1, fibrinogen and lysozyme amyloids. Viscera are particularly affected. There is no involvement of the nervous system. Clinical features include renal amyloidosis resulting in nephrotic syndrome, arterial hypertension, hepatosplenomegaly, cholestasis, petechial skin rash.

  • 配列類似性

    Contains 1 fibrinogen C-terminal domain.

  • ドメイン

    A long coiled coil structure formed by 3 polypeptide chains connects the central nodule to the C-terminal domains (distal nodules). The long C-terminal ends of the alpha chains fold back, contributing a fourth strand to the coiled coil structure.

  • 翻訳後修飾

    The alpha chain is not glycosylated.
    Forms F13A-mediated cross-links between a glutamine and the epsilon-amino group of a lysine residue, forming fibronectin-fibrinogen heteropolymers.
    About one-third of the alpha chains in the molecules in blood were found to be phosphorylated.
    Conversion of fibrinogen to fibrin is triggered by thrombin, which cleaves fibrinopeptides A and B from alpha and beta chains, and thus exposes the N-terminal polymerization sites responsible for the formation of the soft clot. The soft clot is converted into the hard clot by factor XIIIA which catalyzes the epsilon-(gamma-glutamyl)lysine cross-linking between gamma chains (stronger) and between alpha chains (weaker) of different monomers.
    Phosphorylation sites are present in the extracelllular medium.

  • 細胞内局在

    Secreted.
  • Information by UniProt

  • 参照データベース

    see all

  • 別名

    • Ac1873 antibody
    • Fba5e antibody
    • FGA antibody
    • Fib antibody
    • Fib2 antibody
    • FIBA_HUMAN antibody
    • Fibrinogen alpha chain antibody
    • Fibrinogen, A alpha polypeptide antibody
    • Fibrinogen--alpha polypeptide chain antibody
    • Fibrinopeptide A antibody
    see all

画像

  • Flow Cytometry - Anti-Fibrinogen alpha chain antibody [UC45] (ab19079)
    Flow Cytometry - Anti-Fibrinogen alpha chain antibody [UC45] (ab19079)
    Overlay histogram showing THP1 cells stained with ab19079 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab19079, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgM (mu chain) (ab97007) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgM [ICIGM] (ab91545, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

参考文献

ab19079 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab19079 は 8 報の論文で使用されています。

  • Lv LL  et al. Effect of haptoglobin on the treatment of chronic hepatitis B with interferon. Exp Ther Med 18:1417-1425 (2019). PubMed: 31316628
  • Matsuoka A  et al. Novel strategy of ovarian cancer implantation: Pre-invasive growth of fibrin-anchored cells with neovascularization. Cancer Sci 110:2658-2666 (2019). PubMed: 31199029
  • Yeung AM  et al. Fibrin glue inhibits migration of ocular surface epithelial cells. Eye (Lond) 30:1389-1394 (2016). PubMed: 27367746
  • Richens JL  et al. Practical detection of a definitive biomarker panel for Alzheimer's disease; comparisons between matched plasma and cerebrospinal fluid. Int J Mol Epidemiol Genet 5:53-70 (2014). ELISA ; Human . PubMed: 24959311
  • Wu X  et al. Molecular MRI of atherosclerotic plaque progression in an ApoE(-/-) mouse model with a CLT1 peptide targeted macrocyclic Gd(III) chelate. Am J Nucl Med Mol Imaging 3:446-55 (2013). PubMed: 24116353
  • Linch DC  et al. Monoclonal antibodies differentiating between monocytic and nonmonocytic variants of AML. Blood 63:566-73 (1984). PubMed: 6365201
  • Hogg N Human monocytes are associated with the formation of fibrin. J Exp Med 157:473-85 (1983). PubMed: 6822786
  • Hogg N  et al. Monoclonal antibody with specificity for monocytes and neurons. Cell 24:875-84 (1981). PubMed: 6788378

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1-4 of 4 Abreviews or Q&A

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Fibrinogen alpha chain antibody [UC45]

 Excellent
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (The Mouse Arteriovenous Fistula Model)
Antigen retrieval step
None
Permeabilization
No
Specification
The Mouse Arteriovenous Fistula Model
Blocking step
0.4% Casein (in phosphate-buffered saline with stabilizers, surfactant, 0.2% Bronidox L as a preservative) as blocking agent for 10 minute(s) · Concentration: 0.4% · Temperature: RT°C
Fixative
Paraformaldehyde
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Jia Hong Lin

Verified customer

投稿 Dec 14 2016

Immunocytochemistry/ Immunofluorescence abreview for Anti-Fibrin alpha chain antibody [UC45]

 Average
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Rat Cell (islet cell)
Specification
islet cell
Fixative
frozen (OCT)
Permeabilization
No
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 20% · Temperature: 25°C
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Abcam user community

Verified customer

投稿 Feb 20 2012

Question

Has this antibody been tested to cross-react with fibrinogen?

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Answer

Thank you for your enquiry regarding ab19079 and your interest in our products. This antibody reacts very strongly with the alpha chain of human fibrinogen – for further information please see Hogg 1983 paper quoted. I hope this information is useful for you.

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Question

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Answer

Thank you for your enquiry. Following correspondence with the source of this antibody I have determined that this antibody was applied using immunocytochemistry in the following publication: Hogg N Human monocytes are associated with the formation of fibrin. J Exp Med 157:473-85 (1983). I have updated the datasheet with immediate effect. Unfortunately I do not have details of its application by immunohistochemistry. I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

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