Anti-FHL2 抗体 (ab12328)
Key features and details
- Rabbit polyclonal to FHL2
- Suitable for: IP, ICC, IHC-P, WB
- Reacts with: Mouse, Human
- Isotype: IgG
製品の概要
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製品名
Anti-FHL2 antibody
FHL2 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to FHL2 -
由来種
Rabbit -
アプリケーション
適用あり: IP, ICC, IHC-P, WBmore details -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Rat, Rabbit, Horse, Guinea pig, Cow, Dog, Chimpanzee, Rhesus monkey, Gorilla, Chinese hamster, Orangutan, Elephant
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免疫原
Synthetic peptide corresponding to Human FHL2.
Database link: Q14192 -
ポジティブ･コントロール
- WB: HeLa, TCMK-1 and NIH/3T3 whole cell lysates. IP: HeLa cells. IHC-P: Human breast and ovarian carcinoma tissue.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7
Preservative: 0.1% Sodium azide
Constituents: 0.021% PBS, 1.815% Tris, 1.764% Sodium citrate -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
This antibody is affinity purified. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab12328の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IP |
Use a concentration of 1 µg/ml.
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| ICC |
Use a concentration of 1 µg/ml.
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| IHC-P |
1/500 - 1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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| WB |
1/1000 - 1/10000. Detects a band of approximately 32 kDa (predicted molecular weight: 44.6 kDa).
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| 特記事項 |
|---|
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IP
Use a concentration of 1 µg/ml. |
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ICC
Use a concentration of 1 µg/ml. |
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IHC-P
1/500 - 1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
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WB
1/1000 - 1/10000. Detects a band of approximately 32 kDa (predicted molecular weight: 44.6 kDa). |
ターゲット情報
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機能
May function as a molecular transmitter linking various signaling pathways to transcriptional regulation. Negatively regulates the transcriptional repressor E4F1 and may function in cell growth. -
組織特異性
Expressed in skeletal muscle and heart. -
配列類似性
Contains 4 LIM zinc-binding domains. -
ドメイン
The third LIM zinc-binding mediates interaction with E4F1. -
細胞内局在
Cytoplasm. Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 510008 Cow
- Entrez Gene: 2274 Human
- Entrez Gene: 14200 Mouse
- Entrez Gene: 63839 Rat
- Omim: 602633 Human
- SwissProt: Q2KI95 Cow
- SwissProt: Q14192 Human
- SwissProt: O70433 Mouse
see all -
別名
- AAG 11 antibody
- AAG11 antibody
- Aging associated gene 11 antibody
see all
画像
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Western blot - Anti-FHL2 antibody (ab12328)All lanes : Anti-FHL2 antibody (ab12328) at 0.1 µg/ml
Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate
Lane 2 : TCMK-1 (Mouse kidney epithelial cell line) whole cell lysate
Lane 3 : NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate
Lysates/proteins at 50 µg per lane.
Predicted band size: 44.6 kDa
Exposure time: 30 seconds -
Immunoprecipitation - Anti-FHL2 antibody (ab12328)FHL2 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab12328 at 3 µg/mg lysate (different LOT numbers). Western blot was performed from the immunoprecipitate using ab12328 at 1 µg/ml.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FHL2 antibody (ab12328)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma tissue labelling FHL2 with ab12328 at 1/2000 (0.5 µg/ml). Detection: DAB.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FHL2 antibody (ab12328)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling FHL2 with ab12328 at 1/1000 (1µg/ml). Detection: DAB. -
Immunocytochemistry - Anti-FHL2 antibody (ab12328)ICC image of ab12328 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab12328, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab12328 は論文での使用が確認できていません。