Synthetic peptide corresponding to Human FGFR4 aa 24-55 (N terminal) conjugated to Keyhole Limpet Haemocyanin (KLH).
WB: SH-SY5Y, 293 and Raji cell lysates.
ICC/IF: HeLa cells.
IHC-P: Human hepatocarcinoma tissue.
FC: WiDr cells.
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.09% Sodium azide Constituent: PBS
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Protein A purified
This antibody is purified through a protein A column, followed by peptide affinity purification.
Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains. The tyrosine kinase (TK) group is mainly involved in the regulation of cell-cell interactions such as differentiation, adhesion, motility and death. There are currently about 90 TK genes sequenced, 58 are of receptor protein TK (e.g. EGFR, EPH, FGFR, PDGFR, TRK, and VEGFR families), and 32 of cytosolic TK (e.g. ABL, FAK, JAK, and SRC families).
Immunocytochemistry/Immunofluorescence analysis of HeLa cells labelling FGFR4 with ab5481 at 1/200. Cells were fixed with 4% paraformaldehyde for 20 minutes and permeabilized with 0.2% Triton X-100 for 30 minutes. Cells were incubated with the primary antibody for 2 hours at room temperature. An Alexa Fluor® 488 conjugated donkey anti-rabbit IgG (green) was used as the secondary antibody (1/1000, 1 hour). Nuclei were counterstained with Hoechst 33342 (blue) (10 μg/ml, 5 minutes).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human hepatocarcinoma tissue labelling FGFR4 with ab5481. A peroxidase-conjugated anti-rabbit IgG was used as the secondary antibody, followed by AEC staining.
Flow Cytometry - Anti-FGFR4 antibody (ab5481)
Flow cytometry analysis of WiDr cells labelling FGFR4 (green) with ab5481 compared to a negative control. A FITC-conjugated goat anti-rabbit IgG was used as the secondary antibody.