製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to FGFR4
  • 由来種
    Rabbit
  • アプリケーション
    適用あり: ICC/IF, Flow Cyt, WB, IHC-Pmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide corresponding to Human FGFR4 aa 24-55 (N terminal) conjugated to Keyhole Limpet Haemocyanin (KLH).

  • ポジティブ・コントロール
    • WB: SH-SY5Y, 293 and Raji cell lysates. ICC/IF: HeLa cells. IHC-P: Human hepatocarcinoma tissue. FC: WiDr cells.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファー
    Preservative: 0.09% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • 精製度
    Protein A purified
  • 特記事項(精製)
    This antibody is purified through a protein A column, followed by peptide affinity purification.
  • 一次抗体 備考
    Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains. The tyrosine kinase (TK) group is mainly involved in the regulation of cell-cell interactions such as differentiation, adhesion, motility and death. There are currently about 90 TK genes sequenced, 58 are of receptor protein TK (e.g. EGFR, EPH, FGFR, PDGFR, TRK, and VEGFR families), and 32 of cytosolic TK (e.g. ABL, FAK, JAK, and SRC families).
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab5481 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF 1/200.
Flow Cyt 1/10 - 1/50.

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

 

WB 1/1000. Detects a band of approximately 95 kDa (predicted molecular weight: 87 kDa).
IHC-P 1/50 - 1/100.

ターゲット情報

  • 機能
    Receptor for acidic fibroblast growth factor. Does not bind to basic fibroblast growth factor. Binds FGF19.
  • 組織特異性
    Expressed in gastrointestinal epithelial cells, pancreas, and gastric and pancreatic cancer cell lines.
  • 配列類似性
    Belongs to the protein kinase superfamily. Tyr protein kinase family. Fibroblast growth factor receptor subfamily.
    Contains 3 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 1 protein kinase domain.
  • 翻訳後修飾
    Glycosylated.
    Phosphorylated on tyrosine residue (By similarity). Phosphorylation requires the presence of a functional (phosphorylated) FGFR1 and not necessarily by means of FGFR heterodimerization.
  • 細胞内局在
    Membrane. Isoform 2 may be secreted.
  • Information by UniProt
  • 参照データベース
  • 別名
    • CD 334 antibody
    • CD334 antibody
    • CD334 antigen antibody
    • fc13h 10 antibody
    • fc13h10 antibody
    • Fgfr 4 antibody
    • FGFR-4 antibody
    • Fgfr4 antibody
    • FGFR4_HUMAN antibody
    • Fibroblast growth factor receptor 4 antibody
    • Hydroxyaryl protein kinase antibody
    • JTK 2 antibody
    • JTK2 antibody
    • MGC20292 antibody
    • Protein tyrosine kinase antibody
    • TKF antibody
    • Tyrosine kinase related to fibroblast growth factor receptor antibody
    • Tyrosylprotein kinase antibody
    see all

画像

  • All lanes : Anti-FGFR4 antibody (ab5481) at 1/1000 dilution

    Lane 1 : SH-SY5Y cell lysate
    Lane 2 : 293 cell lysate
    Lane 3 : Raji cell lysate

    Lysates/proteins at 35 µg per lane.

    Secondary
    All lanes : HRP-conjugated goat anti-rabbit IgG (H+L) at 1/5000 dilution

    Predicted band size: 87 kDa

  • Anti-FGFR4 antibody (ab5481) + 293 cell lysate at 35 µg

    Predicted band size: 87 kDa

  • Immunocytochemistry/Immunofluorescence analysis of HeLa cells labelling FGFR4 with ab5481 at 1/200. Cells were fixed with 4% paraformaldehyde for 20 minutes and permeabilized with 0.2% Triton X-100 for 30 minutes. Cells were incubated with the primary antibody for 2 hours at room temperature. An Alexa Fluor® 488 conjugated donkey anti-rabbit IgG (green) was used as the secondary antibody (1/1000, 1 hour). Nuclei were counterstained with Hoechst 33342 (blue) (10 μg/ml, 5 minutes).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human hepatocarcinoma tissue labelling FGFR4 with ab5481. A peroxidase-conjugated anti-rabbit IgG was used as the secondary antibody, followed by AEC staining.

  • Flow cytometry analysis of WiDr cells labelling FGFR4 (green) with ab5481 compared to a negative control. A FITC-conjugated goat anti-rabbit IgG was used as the secondary antibody.

参考文献

This product has been referenced in:
  • Chung CP  et al. a-Klotho expression determines nitric oxide synthesis in response to FGF-23 in human aortic endothelial cells. PLoS One 12:e0176817 (2017). WB ; Human . Read more (PubMed: 28463984) »
  • Zhang X  et al. FGF19 genetic amplification as a potential therapeutic target in lung squamous cell carcinomas. Thorac Cancer 8:655-665 (2017). WB . Read more (PubMed: 28906590) »
See all 2 Publications for this product

レビューと Q&A

1-3 of 3 Abreviews or Q&A

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Human Tissue sections (human fetal heart)
Specification
human fetal heart
Fixative
Paraformaldehyde
Permeabilization
Yes - saponin 0.1%
Blocking step
Serum as blocking agent for 45 minute(s) · Concentration: 10% · Temperature: 37°C

Abcam user community

Verified customer

投稿 Mar 11 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (A549 cells)
Loading amount
10 µg
Specification
A549 cells
Gel Running Conditions
Reduced Denaturing (4-12%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

Abcam user community

Verified customer

投稿 Mar 27 2009

Answer

Thank you for your enquiry. Further to correspondence with the source of this antibody unfortunately I have been unable to determine whether the multiple bands that you have been observing is typical of any of these antibodies. As a general rule if a customer has high background on their blot we recommend that they try changing the blocking buffer to BSA, increasing the stringency and duration of the wash conditions and decreasing the concentration of the antibody. Should you continue to obtain these extraneous bands I would greatly appreciate it if you complete our technical questionnaire by clicking on the links below. This will greatly help our technical team determine the steps that you have taken to optimise this antibody. It would be most useful if you could additionally provide us with the LOT numbers and representative images of the blots. I look forward to hearing from you. ab5481 https://www.abcam.com/index.html?section=western&pageconfig=technical&intAbID=5481&mode=questionaire ab10646 https://www.abcam.com/index.html?section=western&pageconfig=technical&intAbID=10646&mode=questionaire ab10647 https://www.abcam.com/index.html?section=western&pageconfig=technical&intAbID=10647&mode=questionaire ab10649 https://www.abcam.com/index.html?section=western&pageconfig=technical&intAbID=10649&mode=questionaire

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

登録