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    fgfr2-antibody-epr5180-ab109372.pdf

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Cardiovascular Angiogenesis Growth Factors FGF FGF Receptors
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遺伝子ノックアウト細胞株 検証済リコンビナントRabMAb

Anti-FGFR2 抗体 [EPR5180] (ab109372)

  • Datasheet
Reviews (1) Submit a question References (8)

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Western blot - Anti-FGFR2 antibody [EPR5180] (ab109372)
  • Western blot - Anti-FGFR2 antibody [EPR5180] (ab109372)
  • Immunoprecipitation - Anti-FGFR2 antibody [EPR5180] (ab109372)
  • Anti-FGFR2 antibody [EPR5180] (ab109372)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR5180] to FGFR2
  • Suitable for: WB, IP
  • Knockout validated
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

こちらの製品もご検討ください

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Chaetocin, histone lysine methyltransferase inhibitor (ab144534)
一次抗体
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Anti-FGFR1 (phospho Y653) antibody [EPR843(N)] (ab173305)
二次抗体
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

関連製品

製品の概要

  • 製品名

    Anti-FGFR2 antibody [EPR5180]
    FGFR2 一次抗体 製品一覧
  • 製品の詳細

    Rabbit monoclonal [EPR5180] to FGFR2
  • 由来種

    Rabbit
  • アプリケーション

    適用あり: WB, IPmore details
    適用なし: Flow Cyt,ICC or IHC-P
  • 種交差性

    交差種: Human
    交差が予測される動物種: Mouse, Rat
  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ・コントロール

    • WB: MCF7, Jurkat, HeLa, K562, and T47-D cell lysates; IP: T-47D whole cell lysate.
  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

製品の特性

  • 製品の状態

    Liquid
  • 保存方法

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • バッファー

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.5% BSA
  • Concentration information loading...
  • 精製度

    Protein A purified
  • ポリ/モノ

    モノクローナル
  • クローン名

    EPR5180
  • アイソタイプ

    IgG
  • 研究分野

    • Cardiovascular
    • Angiogenesis
    • Growth Factors
    • FGF
    • FGF Receptors
    • Signal Transduction
    • Protein Phosphorylation
    • Tyrosine Kinases
    • Receptor Tyrosine Kinases
    • Signal Transduction
    • Growth Factors/Hormones
    • FGF
    • Neuroscience
    • Neurology process
    • Neurogenesis
    • Stem Cells
    • Lineage Markers
    • Ectoderm
    • Cancer
    • Growth factors
    • FGF
    • Cancer
    • Signal transduction
    • Protein phosphorylation
    • Tyrosine kinases
    • Receptor tyrosine kinases
    • Developmental Biology
    • Lineage specification
    • Ectoderm
    • Neuroscience
    • Development

関連製品

  • Alternative Versions

    • Anti-FGFR2 antibody [EPR5180] - BSA and Azide free (ab239970)
  • Compatible Secondaries

    • Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Positive Controls

    • MCF7 membrane extract lysate (ab29539)
    • HeLa membrane extract lysate (ab29547)
    • Jurkat membrane extract lysate (ab30130)
  • Recombinant Protein

    • Recombinant human FGFR2 protein (ab89755)

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab109372の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB (1)
1/1000 - 1/10000. Detects a band of approximately 145 kDa (predicted molecular weight: 92 kDa).
IP
1/10 - 1/100.
特記事項
WB
1/1000 - 1/10000. Detects a band of approximately 145 kDa (predicted molecular weight: 92 kDa).
IP
1/10 - 1/100.
追加情報
Is unsuitable for Flow Cyt,ICC or IHC-P.

ターゲット情報

  • 機能

    Receptor for acidic and basic fibroblast growth factors.
  • 関連疾患

    Defects in FGFR2 are the cause of Crouzon syndrome (CS) [MIM:123500]; also called craniofacial dysostosis type I (CFD1). CS is an autosomal dominant syndrome characterized by craniosynostosis (premature fusion of the skull sutures), hypertelorism, exophthalmos and external strabismus, parrot-beaked nose, short upper lip, hypoplastic maxilla, and a relative mandibular prognathism.
    Defects in FGFR2 are a cause of Jackson-Weiss syndrome (JWS) [MIM:123150]. JWS is an autosomal dominant craniosynostosis syndrome characterized by craniofacial abnormalities and abnormality of the feet: broad great toes with medial deviation and tarsal-metatarsal coalescence.
    Defects in FGFR2 are a cause of Apert syndrome (APRS) [MIM:101200]; also known as acrocephalosyndactyly type 1 (ACS1). APRS is a syndrome characterized by facio-cranio-synostosis, osseous and membranous syndactyly of the four extremities, and midface hypoplasia. The craniosynostosis is bicoronal and results in acrocephaly of brachysphenocephalic type. Syndactyly of the fingers and toes may be total (mitten hands and sock feet) or partial affecting the second, third, and fourth digits. Intellectual deficit is frequent and often severe, usually being associated with cerebral malformations.
    Defects in FGFR2 are a cause of Pfeiffer syndrome (PS) [MIM:101600]; also known as acrocephalosyndactyly type V (ACS5). PS is characterized by craniosynostosis (premature fusion of the skull sutures) with deviation and enlargement of the thumbs and great toes, brachymesophalangy, with phalangeal ankylosis and a varying degree of soft tissue syndactyly. Three subtypes of Pfeiffer syndrome have been described: mild autosomal dominant form (type 1); cloverleaf skull, elbow ankylosis, early death, sporadic (type 2); craniosynostosis, early demise, sporadic (type 3).
    Defects in FGFR2 are the cause of Beare-Stevenson cutis gyrata syndrome (BSCGS) [MIM:123790]. BSCGS is an autosomal dominant condition is characterized by the furrowed skin disorder of cutis gyrata, acanthosis nigricans, craniosynostosis, craniofacial dysmorphism, digital anomalies, umbilical and anogenital abnormalities and early death.
    Defects in FGFR2 are the cause of familial scaphocephaly syndrome (FSPC) [MIM:609579]; also known as scaphocephaly with maxillary retrusion and mental retardation. FSPC is an autosomal dominant craniosynostosis syndrome characterized by scaphocephaly, macrocephaly, hypertelorism, maxillary retrusion, and mild intellectual disability. Scaphocephaly is the most common of the craniosynostosis conditions and is characterized by a long, narrow head. It is due to premature fusion of the sagittal suture or from external deformation.
    Defects in FGFR2 are a cause of lacrimo-auriculo-dento-digital syndrome (LADDS) [MIM:149730]; also known as Levy-Hollister syndrome. LADDS is a form of ectodermal dysplasia, a heterogeneous group of disorders due to abnormal development of two or more ectodermal structures. LADDS is an autosomal dominant syndrome characterized by aplastic/hypoplastic lacrimal and salivary glands and ducts, cup-shaped ears, hearing loss, hypodontia and enamel hypoplasia, and distal limb segments anomalies. In addition to these cardinal features, facial dysmorphism, malformations of the kidney and respiratory system and abnormal genitalia have been reported. Craniosynostosis and severe syndactyly are not observed.
    Defects in FGFR2 are the cause of Antley-Bixler syndrome (ABS) [MIM:207410]. ABS is a multiple congenital anomaly syndrome characterized by craniosynostosis, radiohumeral synostosis, midface hypoplasia, malformed ears, arachnodactyly and multiple joint contractures. ABS is a heterogeneous disorder and occurs with and without abnormal genitalia in both sexes.
  • 配列類似性

    Belongs to the protein kinase superfamily. Tyr protein kinase family. Fibroblast growth factor receptor subfamily.
    Contains 3 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 1 protein kinase domain.
  • 細胞内局在

    Secreted and Cell membrane.
  • Target information above from: UniProt accession P21802 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 参照データベース

    • Entrez Gene: 2263 Human
    • Entrez Gene: 14183 Mouse
    • Entrez Gene: 25022 Rat
    • Omim: 176943 Human
    • SwissProt: P21802 Human
    • SwissProt: P21803 Mouse
    • Unigene: 533683 Human
    • Unigene: 16340 Mouse
    • 別名

      • bacteria-expressed kinase antibody
      • BBDS antibody
      • BEK antibody
      • BEK fibroblast growth factor receptor antibody
      • BFR1 antibody
      • CD332 antibody
      • CD332 antigen antibody
      • CEK3 antibody
      • CFD1 antibody
      • Craniofacial dysostosis 1 antibody
      • ECT1 antibody
      • FGF receptor antibody
      • FGFR 2 antibody
      • FGFR-2 antibody
      • Fgfr2 antibody
      • FGFR2_HUMAN antibody
      • Fibroblast growth factor receptor 2 antibody
      • Hydroxyaryl protein kinase antibody
      • Jackson Weiss syndrome antibody
      • JWS antibody
      • K SAM antibody
      • K-sam antibody
      • Keratinocyte growth factor receptor 2 antibody
      • Keratinocyte growth factor receptor antibody
      • KGFR antibody
      • KSAM antibody
      • protein tyrosine kinase, receptor like 14 antibody
      • soluble FGFR4 variant 4 antibody
      • TK14 antibody
      • TK25 antibody
      see all

    画像

    • Western blot - Anti-FGFR2 antibody [EPR5180] (ab109372)
      Western blot - Anti-FGFR2 antibody [EPR5180] (ab109372)

      Lane 1: Wild-type HAP1 cell lysate (20 µg)
      Lane 2: FGFR2 knockout HAP1 cell lysate (20 µg)
      Lane 3: HeLa cell lysate (20 µg)
      Lane 4: Jurkat cell lysate (20 µg)

      Lanes 1 - 4: Merged signal (red and green). Green - ab109372 observed at 160 kDa. Red - loading control, ab8245, observed at 37 kDa.

      ab109372 was shown to specifically react with FGFR2 when FGFR2 knockout samples were used. Wild-type and FGFR2 knockout samples were subjected to SDS-PAGE.  Ab109372 and ab8245 (loading control to GAPDH) were diluted at 1/1000 and 1/10,000 dilution respectively and incubated overnight at 4C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.

    • Western blot - Anti-FGFR2 antibody [EPR5180] (ab109372)
      Western blot - Anti-FGFR2 antibody [EPR5180] (ab109372)
      All lanes : Anti-FGFR2 antibody [EPR5180] (ab109372) at 1/1000 dilution

      Lane 1 : MCF7 cell lysate
      Lane 2 : Jurkat cell lysate
      Lane 3 : HeLa cell lysate
      Lane 4 : K562 cell lysate
      Lane 5 : T47-D cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 92 kDa
      Observed band size: 145 kDa why is the actual band size different from the predicted?

    • Immunoprecipitation - Anti-FGFR2 antibody [EPR5180] (ab109372)
      Immunoprecipitation - Anti-FGFR2 antibody [EPR5180] (ab109372)

      Purified ab109372 at 1/40 dilution (2µg) immunoprecipitating FGFR2 in T-47D whole cell lysate.
      Lane 1 (input): T-47D (Human ductal breast epithelial tumor epithelial cell) whole cell lysate 10µg
      Lane 2 (+): ab109372 + T-47D whole cell lysate.
      Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab109372 in T-47D whole cell lysate.
      VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/1000 dilution) was used for Western blotting.
      Blocking Buffer and concentration: 5% NFDM/TBST.
      Diluting buffer and concentration: 5% NFDM/TBST.
      Observed band size: 145 kDa

    • Anti-FGFR2 antibody [EPR5180] (ab109372)
      Anti-FGFR2 antibody [EPR5180] (ab109372)

    プロトコール

    • Western blot protocols
    • Immunoprecipitation protocols

    Click here to view the general protocols

    データシートおよび資料

    • Datasheet download

      Download

    参考文献 (8)

    ab109372 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab109372 は 8 報の論文で使用されています。

    • Zhou BY  et al. Nintedanib inhibits keloid fibroblast functions by blocking the phosphorylation of multiple kinases and enhancing receptor internalization. Acta Pharmacol Sin 41:1234-1245 (2020). PubMed: 32327724
    • ZhuGe DL  et al. Fibroblast growth factor 2 exacerbates inflammation in adipocytes through NLRP3 inflammasome activation. Arch Pharm Res 43:1311-1324 (2020). PubMed: 33245516
    • Wu J  et al. Estrogen-Induced Stromal FGF18 Promotes Proliferation and Invasion of Endometrial Carcinoma Cells Through ERK and Akt Signaling. Cancer Manag Res 12:6767-6777 (2020). PubMed: 32801905
    • Ray AT  et al. FGF signaling regulates development by processes beyond canonical pathways. Genes Dev 34:1735-1752 (2020). PubMed: 33184218
    • Lee KW  et al. FGF11 influences 3T3-L1 preadipocyte differentiation by modulating the expression of PPAR? regulators. FEBS Open Bio 9:769-780 (2019). PubMed: 30984550
    • Kang J  et al. A Selective FGFR inhibitor AZD4547 suppresses RANKL/M-CSF/OPG-dependent ostoclastogenesis and breast cancer growth in the metastatic bone microenvironment. Sci Rep 9:8726 (2019). PubMed: 31217507
    • Xu L  et al. Gambogenic acid inhibits fibroblast growth factor receptor signaling pathway in erlotinib-resistant non-small-cell lung cancer and suppresses patient-derived xenograft growth. Cell Death Dis 9:262 (2018). PubMed: 29449529
    • Lv XM  et al. Neotuberostemonine inhibits the differentiation of lung fibroblasts into myofibroblasts in mice by regulating HIF-1a signaling. Acta Pharmacol Sin N/A:N/A (2018). PubMed: 29645000

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    Western blot abreview for Anti-FGFR2 antibody [EPR5180]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Dog Cell lysate - whole cell (Human cervical cancer (HeLa), Canine osteosarcoma)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    30 µg
    Specification
    Human cervical cancer (HeLa), Canine osteosarcoma
    Blocking step
    T20 Super Block as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 23°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    投稿 Apr 16 2018

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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