Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Intestine)
Antigen retrieval step
Enzymatic - Buffer/Enzyme Used: 0.25% Trypsin/EDTA
Permeabilization
Yes - 0.3% Triton X100
Specification
Intestine
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 22°C
Fixative
Formalin
Other product details
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: PBS 0.025% TritonX100, 1% BSA
Dilution
1/200
Secondary antibody
Dilution
1/1000
Name
Non-Abcam antibody was used: Goat anti-Rabbit IgG (H+L) Highly Cross-Adsorbed S
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Additional data
Abcam response
Thank you for your valuable feedback. We do not validate this antibody in IHC-P and it seems that this antibody does not work well under these experimental conditions. Thank you for your time - we hope that this information will be helpful for future customers.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
投稿 Dec 24 2020