製品の概要

  • 製品名
    Anti-FGFR1 antibody [M5G10]
    FGFR1 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [M5G10] to FGFR1
  • 由来種
    Mouse
  • 特異性
    Reacts with both alpha and beta isoforms.
  • アプリケーション
    適用あり: ICC/IF, IP, IHC-P, WB, Flow Cytmore details
  • 種交差性
    交差種: Mouse, Human
  • 免疫原

    Recombinant human ectodomain of FGFr1 beta expressed in E. coli beginning with proline 23; antigen contained NH2-terminal gly-ser-pro-gly-ile and COOH-terminal glu-phe sequences.

  • エピトープ
    Epitope is within the sequence His 241 and Val 267 between Ig loops II and III. Epitope is masked in undenatured FGFr1a.
  • ポジティブ・コントロール
    • This antibody gave a positive result when used in the following formaldehyde/methanol fixed cell lines: SKNSH.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab824 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use a concentration of 5 µg/ml.
IP Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Detects a band of approximately 105 kDa.
Flow Cyt Use 1µg for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

ターゲット情報

  • 機能
    Receptor for basic fibroblast growth factor. Receptor for FGF23 in the presence of KL (By similarity). A shorter form of the receptor could be a receptor for FGF1 (aFGF).
  • 組織特異性
    Detected in astrocytoma, neuroblastoma and adrenal cortex cell lines. Some isoforms are detected in foreskin fibroblast cell lines, however isoform 17, isoform 18 and isoform 19 are not detected in these cells.
  • 関連疾患
    Defects in FGFR1 are a cause of Pfeiffer syndrome (PS) [MIM:101600]; also known as acrocephalosyndactyly type V (ACS5). PS is characterized by craniosynostosis (premature fusion of the skull sutures) with deviation and enlargement of the thumbs and great toes, brachymesophalangy, with phalangeal ankylosis and a varying degree of soft tissue syndactyly.
    Defects in FGFR1 are a cause of idiopathic hypogonadotropic hypogonadism (IHH) [MIM:146110]. IHH is defined as a deficiency of the pituitary secretion of follicle-stimulating hormone and luteinizing hormone, which results in the impairment of pubertal maturation and of reproductive function.
    Defects in FGFR1 are the cause of Kallmann syndrome type 2 (KAL2) [MIM:147950]; also known as hypogonadotropic hypogonadism and anosmia. Anosmia or hyposmia is related to the absence or hypoplasia of the olfactory bulbs and tracts. Hypogonadism is due to deficiency in gonadotropin-releasing hormone and probably results from a failure of embryonic migration of gonadotropin-releasing hormone-synthesizing neurons. In some cases, midline cranial anomalies (cleft lip/palate and imperfect fusion) are present and anosmia may be absent or inconspicuous.
    Defects in FGFR1 are the cause of osteoglophonic dysplasia (OGD) [MIM:166250]; also known as osteoglophonic dwarfism. OGD is characterized by craniosynostosis, prominent supraorbital ridge, and depressed nasal bridge, as well as by rhizomelic dwarfism and nonossifying bone lesions. Inheritance is autosomal dominant.
    Defects in FGFR1 are the cause of trigonocephaly non-syndromic (TRICEPH) [MIM:190440]; also known as metopic craniosynostosis. The term trigonocephaly describes the typical keel-shaped deformation of the forehead resulting from premature fusion of the frontal suture. Trigonocephaly may occur also as a part of a syndrome.
    Note=A chromosomal aberration involving FGFR1 may be a cause of stem cell leukemia lymphoma syndrome (SCLL). Translocation t(8;13)(p11;q12) with ZMYM2. SCLL usually presents as lymphoblastic lymphoma in association with a myeloproliferative disorder, often accompanied by pronounced peripheral eosinophilia and/or prominent eosinophilic infiltrates in the affected bone marrow.
    Note=A chromosomal aberration involving FGFR1 may be a cause of stem cell myeloproliferative disorder (MPD). Translocation t(6;8)(q27;p11) with FGFR1OP. Insertion ins(12;8)(p11;p11p22) with FGFR1OP2. MPD is characterized by myeloid hyperplasia, eosinophilia and T-cell or B-cell lymphoblastic lymphoma. In general it progresses to acute myeloid leukemia. The fusion proteins FGFR1OP2-FGFR1, FGFR1OP-FGFR1 or FGFR1-FGFR1OP may exhibit constitutive kinase activity and be responsible for the transforming activity.
    Note=A chromosomal aberration involving FGFR1 may be a cause of stem cell myeloproliferative disorder (MPD). Translocation t(8;9)(p12;q33) with CEP110. MPD is characterized by myeloid hyperplasia, eosinophilia and T-cell or B-cell lymphoblastic lymphoma. In general it progresses to acute myeloid leukemia. The fusion protein CEP110-FGFR1 is found in the cytoplasm, exhibits constitutive kinase activity and may be responsible for the transforming activity.
  • 配列類似性
    Belongs to the protein kinase superfamily. Tyr protein kinase family. Fibroblast growth factor receptor subfamily.
    Contains 3 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 1 protein kinase domain.
  • 翻訳後修飾
    Binding of FGF1 and heparin promotes autophosphorylation on tyrosine residues and activation of the receptor.
  • 細胞内局在
    Membrane. Nucleus. Cytoplasm. Cytoplasmic vesicle
  • Information by UniProt
  • 参照データベース
  • 別名
    • Basic fibroblast growth factor receptor 1 antibody
    • bFGF-R-1 antibody
    • BFGFR antibody
    • CD331 antibody
    • CEK antibody
    • FGFBR antibody
    • FGFR 1 antibody
    • FGFR-1 antibody
    • FGFR1 antibody
    • FGFR1/PLAG1 fusion antibody
    • FGFR1_HUMAN antibody
    • fibroblast growth factor receptor 1 antibody
    • FLG antibody
    • FLT-2 antibody
    • FLT2 antibody
    • Fms-like gene antibody
    • Fms-like tyrosine kinase 2 antibody
    • fms-related tyrosine kinase 2 antibody
    • HBGFR antibody
    • heparin-binding growth factor receptor antibody
    • HH2 antibody
    • HRTFDS antibody
    • hydroxyaryl-protein kinase antibody
    • KAL2 antibody
    • N-SAM antibody
    • OGD antibody
    • Proto-oncogene c-Fgr antibody
    see all

画像

  • ICC/IF image of ab824 stained SKNSH cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab824 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- mouse (ab96879) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Overlay histogram showing MCF7 cells stained with ab824 (red line). The cells were fixed with 80% methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab824, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

    Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.
  • All lanes : Anti-FGFR1 antibody [M5G10] (ab824) at 1 µg/ml

    Lane 1 : Recombinant human alpha FGFR1
    Lane 2 : Recombinant human beta FGFR1
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human normal salivary gland labelling FGFR1 with ab824 at 10µg/ml.

参考文献

This product has been referenced in:
  • Rubert Pérez CM  et al. Mimicking the Bioactivity of Fibroblast Growth Factor-2 Using Supramolecular Nanoribbons. ACS Biomater Sci Eng 3:2166-2175 (2017). WB ; Human . Read more (PubMed: 28920077) »
  • Tomiyama K  et al. Relevant use of Klotho in FGF19 subfamily signaling system in vivo. Proc Natl Acad Sci U S A 107:1666-71 (2010). WB, IP ; Mouse . Read more (PubMed: 20080590) »
See all 4 Publications for this product

レビューと Q&A

1-10 of 15 Abreviews or Q&A

Answer

Thank you for contacting Abcam.

Thank you for pointing out the error in figure legends for ab824 and ab79976. The flow cytometry figure legends for the two antibodies have been switched on the online datasheet to correspond with the correct antibody.
ab824: (https://www.abcam.com/fgfr1-antibody-m5g10-ab824.html)

ab824 is specifically raised against the ectodomain FGFR1 beta isoform. Thus, the statement in the epitope description that the "epitope is masked in undenatured FGFr1a" refers to the specificity of this antibody for the FGFR1 beta isoform.

Below are some general troubleshooting tips for flow cytometry, if you are experiencing issues with ab824 please reply with further sample, protocol and troubleshooting steps that have been taken. These will not only help me to fully understand the problem but are essential should further testing of this product be needed as well.
https://www.abcam.com/index.html?pageconfig=resource&rid=11443

Free Rabbit monoclonal antibody with any purchase of a primary antibody, while stocks last! Quote “RABMAB-XBSMG” in your next primary antibody order. For more information, visit the following link: https://www.abcam.com/index.html?pageconfig=resource&rid=15447

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Answer

Thanks for submitting your Abreview.

As yourated your experience with this antibody as 'Inconclusive' I just wanted to let you know that if you feel the antibody is not working as expected I can offer a replacement of any primary antibody, credit or refund. Alternatively, I'm happy to help you troubleshoot this issue as well.

Please let me know if you have any questions. If you would like a replacement, please pass along the new product number you are interested in as well as the PO or Abcan order reference number associated with this purchase of ab824.

Thanks very much. I look forward to hearing from you.

Read More
Application
Western blot
Sample
Human Cell lysate - whole cell (Cancer cell lines)
Loading amount
15 µg
Specification
Cancer cell lines
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: RT°C

Ms. Valentina André

Verified customer

投稿 Sep 28 2012

Answer

Thanks for your email.
Earlier today I sent you and email containing instructions on returning an antibody to Abcam. Below, please find those same instructions:
===========
Included in this email are the instructions for returning your item/s to Abcam. When you return the goods, please ensure that you follow the procedures as below:
- Return in the original Abcam box with the cooler box and ice pack (or similar) within 7 days from today.
- Return all documents (or copies of) that arrived with the product (if they’re available – should be invoice, delivery note and datasheet)
- Please return via an overnight courier such as UPS, DHL, or FEDEX.
- Ship to the following address:
Attn: Returns ORDER REF: (_____)
Abcam Inc
One Kendall Square
Bldg 200, Suite B2304
Cambridge, MA 02139
Please be advised that a 20% restock fee will apply to this order. The most up-to-date information regarding your order, invoice, and credit note status is available online in your Abcam account information. If your order was placed by credit card, your refund will be issued to your credit card. If your order was placed by Purchase Order, all customers must submit a credit note with their invoice to their Accounts Payable department to be handled. The Accounts Payable must have a copy of the credit note so the original invoice can be updated to reflect the correct amount owed.
If you have any other questions please do not hesitate to contact us!
Email: US.orders@Abcam.com
Phone: 888-77-ABCAM(22226)
Fax: 866 739 9884
Customer Service hours of operation:
Monday-Friday 8:30 AM- 9:00PM EST

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Answer

Thank you for contacting Abcam. Included in this email are the instructions for returning your item/s to Abcam. When you return the goods, please ensure that you follow the procedures as below:
- Return in the original Abcam box with the cooler box and ice pack (or similar) within 7 days from today.
- Return all documents (or copies of) that arrived with the product (if they’re available – should be invoice, delivery note and datasheet)
- Please return via an overnight courier such as UPS, DHL, or FEDEX.
- Ship to the following address:
Attn: Returns ORDER REF: (_____)
Abcam Inc
One Kendall Square
Bldg 200, Suite B2304
Cambridge, MA 02139
Please be advised that a 20% restock fee will apply to this order. The most up-to-date information regarding your order, invoice, and credit note status is available online in your Abcam account information. If your order was placed by credit card, your refund will be issued to your credit card. If your order was placed by Purchase Order, all customers must submit a credit note with their invoice to their Accounts Payable department to be handled. The Accounts Payable must have a copy of the credit note so the original invoice can be updated to reflect the correct amount owed.
If you have any other questions please do not hesitate to contact us!
Email: US.orders@Abcam.com
Phone: 888-77-ABCAM(22226)
Fax: 866 739 9884
Customer Service hours of operation:
Monday-Friday 8:30 AM- 9:00PM EST

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Question
Answer

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacements on the following order numbers:
You will receive ab7192 to replace ab824
. You should receive this on Friday March 2.
You will receive ab119237 to replace ab10648.
You should receive this on or around Tuesday March 7 (it is back-ordered).
To check the status of the order please contact our Customer Service team and reference this number.
Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.
I wish you the best of luck with your research.

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Answer

Thanks for your reply.
I'll just need to locate your order information for ab10648 and ab824 on our system so I can process the replacement order.
If you could provide me with:
1) The PO number or Abcam order reference number associated with this order.
2) The catalog numbers of the two antibodies you would like as replacements.
Thanks!

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Answer

I would be happy to send along replacement antibodies to replace those not working as expected.
Based on your last reply, it seemed that ab10648 and ab824 were not detecting protein in the expected subcellular region. The report in your email also noted ab65973 was detecting protein in the cytoplasm and nucleus which is expected.
Is this assessment correct?

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Answer

Thank you for the information.
You are correct that ab10648 and ab824 should be staining the cell surface. ab65973, which stains FGF basic, is expected to be nuclear and/or cytoplasmic.
Have you stained these same tissue sections successfully with any other antibodies? It is quite odd that 3 different antibodies would not be working as expected.

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Answer

Thank you for your enquiry.
Anti-FGFR2 antibody (ab10648) = cell-surface receptor for fibroblast growth factors
Anti-FGFR1 antibody [M5G10] (ab824) = cell-surface receptor for fibroblast growth factors
Anti-FGF basic antibody - Aminoterminal end (ab65973) = can be nuclear or cytoplasmic, depending on isoform
I hope this is helpful. Please contact us again if you have any further questions.

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1-10 of 15 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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