Anti-FGFR1 alpha 抗体 [M2F12] (ab829)
Key features and details
- Mouse monoclonal [M2F12] to FGFR1 alpha
- Suitable for: ICC/IF
- Reacts with: Human
- Isotype: IgG2a
製品の概要
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製品名
Anti-FGFR1 alpha antibody [M2F12]
FGFR1 alpha 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [M2F12] to FGFR1 alpha -
由来種
Mouse -
特異性
Reacts with alpha isoform of FRFR1. -
アプリケーション
適用あり: ICC/IFmore details -
種交差性
交差種: Human -
免疫原
Recombinant human ectodomain of FGFr1a expressed in E. coli beginning with pro23; antigen contained NH2-terminal gly-ser-pro-gly-ile and COOH-terminal glu-phe sequences.
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エピトープ
NH2-terminus of unique NH2-terminal Ig loop of FGFr1. Epitope is within the sequence between glu30 and ala74 of FGFr1a. -
ポジティブ・コントロール
- IHC: Rat prostate tissue.
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特記事項
This product was changed from ascites to tissue culture supernatant on 19/12/2018. Please note that the dilutions may need to be adjusted accordingly. If you have any questions please do not hesitate to contact our scientific support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.40
Constituent: PBS -
Concentration information loading...
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精製度
Protein G purified -
特記事項(精製)
Purified from TCS -
ポリ/モノ
モノクローナル -
クローン名
M2F12 -
アイソタイプ
IgG2a -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab829の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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ICC/IF |
Use a concentration of 10 µg/ml.
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特記事項 |
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ICC/IF
Use a concentration of 10 µg/ml. |
ターゲット情報
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関連性
FGFR1 (fibroblast growth factor receptor 1) is a member of the fibroblast growth factor receptor family and contains an Ig-like domain and a tyrosine kinase domain. This receptor has multiple isoforms and is a Type I membrane protein. FGFR1 is widely expressed, with distinct isoforms expressed in specific tissues. FGFR1 binds fibroblast growth factor and induces mitogenesis and cellular differentiation. Defects in FGFR1 result in Pfeiffer syndrome associated with craniosynostosis. FGFR1 can be modified by phosphorylation and can bind basic/acidic fibroblast factor depending on the receptor isoform. FGFR1 has been shown to interact with N-cadherin and NCAM. -
細胞内局在
Cell Membrane -
参照データベース
- Entrez Gene: 2260 Human
- Omim: 136350 Human
- SwissProt: P11362 Human
- Unigene: 264887 Human
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別名
- BFGFR antibody
- CD331 antibody
- CEK antibody
see all
画像
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ICC/IF image of ab829 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab829, 10µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
This image was generated using the ascites version of the product.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (12)
ab829 は 12 報の論文で使用されています。
- Formisano L et al. Aberrant FGFR signaling mediates resistance to CDK4/6 inhibitors in ER+ breast cancer. Nat Commun 10:1373 (2019). PubMed: 30914635
- Zhang GY et al. Thyroxine (T4) may promote re-epithelialisation and angiogenesis in wounded human skin ex vivo. PLoS One 14:e0212659 (2019). PubMed: 30925152
- Voytyuk I et al. BACE2 distribution in major brain cell types and identification of novel substrates. Life Sci Alliance 1:e201800026 (2018). PubMed: 30456346
- Mao Y et al. VEGF-A/VEGFR-2 and FGF-2/FGFR-1 but not PDGF-BB/PDGFR-ß play important roles in promoting immature and inflammatory intraplaque angiogenesis. PLoS One 13:e0201395 (2018). PubMed: 30125282
- Nakamichi M et al. Basic Fibroblast Growth Factor Induces Angiogenic Properties of Fibrocytes to Stimulate Vascular Formation during Wound Healing. Am J Pathol 186:3203-3216 (2016). PubMed: 27773739