リコンビナントRabMAb

Anti-FGF21 抗体 [EPR8314(2)] - BSA and Azide free (ab271925)

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Western blot - Anti-FGF21 antibody [EPR8314(2)] - BSA and Azide free (ab271925)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FGF21 antibody [EPR8314(2)] - BSA and Azide free (ab271925)
  • Western blot - Anti-FGF21 antibody [EPR8314(2)] - BSA and Azide free (ab271925)
  • Western blot - Anti-FGF21 antibody [EPR8314(2)] - BSA and Azide free (ab271925)
  • Anti-FGF21 antibody [EPR8314(2)] - BSA and Azide free (ab271925)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR8314(2)] to FGF21 - BSA and Azide free
  • Suitable for: IHC-P, WB
  • Reacts with: Mouse, Rat, Human

製品の概要

  • 製品名

    Anti-FGF21 antibody [EPR8314(2)] - BSA and Azide free
    FGF21 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR8314(2)] to FGF21 - BSA and Azide free

  • 由来種

    Rabbit

  • 特異性

    The immunogen used for this product shares 6 continuous identical amino acids with SIKE1. Cross-reactivity with this protein has not been confirmed experimentally.

    Expression levels of the target protein vary with sample type and some optimisation may be required (PMID: 27285327).  For western blot using cell lines, it may be necessary to collect cell culture supernatant for endogenous FGF21 detection as the target protein is readily secreted (PMID: 24041694; PMID: 26691139).

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • Recombinant human FGF21 + IgG1 fusion protein (Fc Chimera Active) (ab108556) can be used as a positive control in WB. WB: Human fetal liver lysate, mouse spleen, rat spleen. IHC-P: Human stomach

  • 特記事項

    ab271925 is the carrier-free version of ab171941. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

アプリケーション

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab271925 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

アプリケーション Species
IHC-P
Human
WB
Human
アプリケーション Abreviews 特記事項
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

See IHC&nbsp;antigen retrieval protocols.

 
WB
Use at an assay dependent concentration. Predicted molecular weight: 22 kDa.
特記事項
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

See IHC&nbsp;antigen retrieval protocols.

 
WB
Use at an assay dependent concentration. Predicted molecular weight: 22 kDa.
  • 追加情報
    Is unsuitable for ICC/IF.

    • ターゲット情報

    画像

    • Western blot - Anti-FGF21 antibody [EPR8314(2)] - BSA and Azide free (ab271925)
      Western blot - Anti-FGF21 antibody [EPR8314(2)] - BSA and Azide free (ab271925)
      Anti-FGF21 antibody [EPR8314(2)] (ab171941) at 1/5000 dilution + Human fetal liver tissue lysate at 20 µg

      Secondary
      HRP goat anti-rabbit (H+L) at 1/1000 dilution

      Predicted band size: 22 kDa



      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab171941).

      Blocking buffer: 5% NFDM/TBST

      Dilution buffer: 5% NFDM/TBST

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FGF21 antibody [EPR8314(2)] - BSA and Azide free (ab271925)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FGF21 antibody [EPR8314(2)] - BSA and Azide free (ab271925)

      Immunohistochemical staining of paraffin embedded human stomach with purified ab171941 at a working dilution of 1/250. The secondary antibody used is ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L), at a dilution of 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab171941).

    • Western blot - Anti-FGF21 antibody [EPR8314(2)] - BSA and Azide free (ab271925)
      Western blot - Anti-FGF21 antibody [EPR8314(2)] - BSA and Azide free (ab271925)
      Anti-FGF21 antibody [EPR8314(2)] (ab171941) at 1/5000 dilution + Rat spleen tissue lysate at 20 µg

      Secondary
      HRP goat anti-rabbit (H+L) at 1/1000 dilution

      Predicted band size: 22 kDa



      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab171941).

      Blocking buffer: 5% NFDM/TBST

      Dilution buffer: 5% NFDM/TBST

    • Western blot - Anti-FGF21 antibody [EPR8314(2)] - BSA and Azide free (ab271925)
      Western blot - Anti-FGF21 antibody [EPR8314(2)] - BSA and Azide free (ab271925)
      Anti-FGF21 antibody [EPR8314(2)] (ab171941) at 1/1000 dilution + Mouse spleen tissue lysate at 10 µg

      Secondary
      HRP goat anti-rabbit (H+L) at 1/1000 dilution

      Predicted band size: 22 kDa



      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab171941).

      Blocking buffer: 5% NFDM/TBST

      Dilution buffer: 5% NFDM/TBST

    • Anti-FGF21 antibody [EPR8314(2)] - BSA and Azide free (ab271925)
      Anti-FGF21 antibody [EPR8314(2)] - BSA and Azide free (ab271925)

    プロトコール

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    参考文献 (0)

    ab271925 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab271925 は論文での使用が確認できていません。

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