Anti-FGF1 抗体 [EPR19989] (ab207321)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR19989] to FGF1
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
製品の概要
-
製品名
Anti-FGF1 antibody [EPR19989]
FGF1 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR19989] to FGF1 -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Recombinant full length protein. This information is proprietary to Abcam and/or its suppliers.
-
ポジティブ・コントロール
- WB: Human fetal kidney, fetal brain and glioma lysates; U-87 MG and Neuro-2a whole cell lysates; Mouse spinal cord, heart and kidney lysates; Rat heart lysate.
-
特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading...
-
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR19989 -
アイソタイプ
IgG -
研究分野
関連製品
-
Alternative Versions
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab207321の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
WB |
1/1000. Detects a band of approximately 16,18 kDa (predicted molecular weight: 17 kDa).
|
特記事項 |
---|
WB
1/1000. Detects a band of approximately 16,18 kDa (predicted molecular weight: 17 kDa). |
ターゲット情報
-
機能
The heparin-binding fibroblast growth factors play important roles in the regulation of cell survival, cell division, angiogenesis, cell differentiation and cell migration. They are potent mitogens in vitro. -
配列類似性
Belongs to the heparin-binding growth factors family. -
細胞内局在
Secreted. Cytoplasm. Cytoplasm > cell cortex. Lacks a cleavable signal sequence. Within the cytoplasm, it is transported to the cell membrane and then secreted by a non-classical pathway that requires Cu(2+) ions and S100A13. Secreted in a complex with SYT1. - Information by UniProt
-
参照データベース
- Entrez Gene: 2246 Human
- Entrez Gene: 14164 Mouse
- Entrez Gene: 25317 Rat
- Omim: 131220 Human
- SwissProt: P05230 Human
- SwissProt: P61148 Mouse
- SwissProt: P61149 Rat
- Unigene: 483635 Human
see all -
別名
- Acidic fibroblast growth factor antibody
- aFGF antibody
- Beta endothelial cell growth factor antibody
see all
画像
-
All lanes : Anti-FGF1 antibody [EPR19989] (ab207321) at 1/1000 dilution
Lane 1 : U-87 MG (Human glioblastoma-astrocytoma epithelial cell line) whole cell lysate
Lane 2 : Human fetal kidney lysate
Lane 3 : Human fetal brain lysate
Lane 4 : Neuro-2a (Mouse neuroblastoma cell line) whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution
Predicted band size: 17 kDa
Observed band size: 16,18 kDa why is the actual band size different from the predicted?Blocking/Dilution buffer: 5% NFDM/TBST.
Exposure times: Lane 1: 1 second; Lane 2/4: 5 seconds; Lane 3: 3 seconds.
The observed molecular weight is consistent with the literature (PMID: 16091423).
-
All lanes : Anti-FGF1 antibody [EPR19989] (ab207321) at 1/1000 dilution
Lane 1 : Human glioma lysate
Lane 2 : Mouse spinal cord lysate
Lane 3 : Mouse heart lysate
Lane 4 : Mouse kidney lysate
Lane 5 : Rat heart lysate
Lysates/proteins at 10 µg per lane.
Secondary
Lane 1 : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/4000 dilution
Lanes 2-5 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 17 kDa
Observed band size: 16,18 kDa why is the actual band size different from the predicted?Blocking/Dilution buffer: 5% NFDM/TBST.
Exposure times: Lane 1/2/5: 3 minutes; Lane 3: 10 seconds; Lane 4: 15 seconds.
The observed molecular weight is consistent with the literature (PMID: 16091423).
データシートおよび資料
-
SDS download
-
Datasheet download
Certificate of Compliance
参考文献 (5)
ab207321 は 5 報の論文で使用されています。
- Subbanna S & Basavarajappa BS Binge-like Prenatal Ethanol Exposure Causes Impaired Cellular Differentiation in the Embryonic Forebrain and Synaptic and Behavioral Defects in Adult Mice. Brain Sci 12:N/A (2022). PubMed: 35741678
- ZhuGe DL et al. Fibroblast growth factor 2 exacerbates inflammation in adipocytes through NLRP3 inflammasome activation. Arch Pharm Res 43:1311-1324 (2020). PubMed: 33245516
- Yoo M et al. Persistence of Fear Memory Depends on a Delayed Elevation of BAF53b and FGF1 Expression in the Lateral Amygdala. J Neurosci 40:7133-7141 (2020). PubMed: 32817243
- Wang D et al. FGF1?HBS ameliorates chronic kidney disease via PI3K/AKT mediated suppression of oxidative stress and inflammation. Cell Death Dis 10:464 (2019). PubMed: 31189876
- Liang G et al. Fibroblast growth factor 1 ameliorates diabetic nephropathy by an anti-inflammatory mechanism. Kidney Int 93:95-109 (2018). IHC-P, IHC-Fr . PubMed: 28750927