Anti-FGF1 抗体 (ab9588)
Key features and details
- Rabbit polyclonal to FGF1
- Suitable for: Sandwich ELISA, WB, ELISA, ICC/IF, IHC-Fr, Neutralising
- Reacts with: Rat, Human
- Isotype: IgG
製品の概要
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製品名
Anti-FGF1 antibody
FGF1 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to FGF1 -
由来種
Rabbit -
アプリケーション
適用あり: Sandwich ELISA, WB, ELISA, ICC/IF, IHC-Fr, Neutralisingmore details -
種交差性
交差種: Rat, Human -
免疫原
Recombinant full length protein corresponding to Human FGF1. Highly pure (>98%) recombinant FGF1 (human Fibroblast Growth Factor-acidic).
Database link: P05230 -
ポジティブ･コントロール
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特記事項
This product is no longer batch tested in IHC, for an IHC validated antibody please see ab179455
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Lyophilized:Reconstitute with 200µl of sterile water. -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
軽鎖の種類
unknown -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab9588の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| Sandwich ELISA |
Use at an assay dependent concentration.
To detect human FGF1 by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.25 – 1.0 μg/ml of ab83163 is required. This biotinylated polyclonal antibody, in conjunction with ab83163 as a capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant human FGF1. |
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| WB | (1) |
Use at an assay dependent concentration.
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| ELISA |
Use at an assay dependent concentration.
|
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| ICC/IF |
Use at an assay dependent concentration.
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| IHC-Fr | (1) |
Use at an assay dependent concentration.
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| Neutralising |
Use at an assay dependent concentration.
To yield one-half maximal inhibition [ND50] of the biological activity of hFGF1 (30.0 ng/ml), a concentration of 0.46 - 0.9 µg/ml of this antibody is required. |
| 特記事項 |
|---|
|
Sandwich ELISA
Use at an assay dependent concentration. To detect human FGF1 by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.25 – 1.0 μg/ml of ab83163 is required. This biotinylated polyclonal antibody, in conjunction with ab83163 as a capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant human FGF1. |
|
WB
Use at an assay dependent concentration. |
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ELISA
Use at an assay dependent concentration. |
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ICC/IF
Use at an assay dependent concentration. |
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IHC-Fr
Use at an assay dependent concentration. |
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Neutralising
Use at an assay dependent concentration. To yield one-half maximal inhibition [ND50] of the biological activity of hFGF1 (30.0 ng/ml), a concentration of 0.46 - 0.9 µg/ml of this antibody is required. |
ターゲット情報
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機能
The heparin-binding fibroblast growth factors play important roles in the regulation of cell survival, cell division, angiogenesis, cell differentiation and cell migration. They are potent mitogens in vitro. -
配列類似性
Belongs to the heparin-binding growth factors family. -
細胞内局在
Secreted. Cytoplasm. Cytoplasm > cell cortex. Lacks a cleavable signal sequence. Within the cytoplasm, it is transported to the cell membrane and then secreted by a non-classical pathway that requires Cu(2+) ions and S100A13. Secreted in a complex with SYT1. - Information by UniProt
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参照データベース
- Entrez Gene: 2246 Human
- Entrez Gene: 25317 Rat
- Omim: 131220 Human
- SwissProt: P05230 Human
- SwissProt: P61149 Rat
- Unigene: 483635 Human
- Unigene: 88013 Rat
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別名
- Acidic fibroblast growth factor antibody
- aFGF antibody
- Beta endothelial cell growth factor antibody
see all
画像
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Sandwich ELISA - Anti-FGF1 antibody (ab9588)
ab9588 detecting FGF-1 in sELISA
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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Datasheet download
参考文献 (10)
ab9588 は 10 報の論文で使用されています。
- Mi S et al. miR-188-3p Inhibits Vascular Smooth Muscle Cell Proliferation and Migration by Targeting Fibroblast Growth Factor 1 (FGF1). Med Sci Monit 26:e924394 (2020). PubMed: 33020467
- Wang M et al. Morphine post-conditioning-induced up-regulation of lncRNA TINCR protects cardiomyocytes from ischemia-reperfusion injury via inhibiting degradation and ubiquitination of FGF1. QJM 113:859-869 (2020). PubMed: 32176291
- Lopatina T et al. Extracellular vesicles from human liver stem cells inhibit tumor angiogenesis. Int J Cancer 144:322-333 (2019). PubMed: 30110127
- Xue YN et al. LncRNA TUG1 regulates FGF1 to enhance endothelial differentiation of adipose-derived stem cells by sponging miR-143. J Cell Biochem 120:19087-19097 (2019). PubMed: 31264280
- Li L et al. miRNA-210-3p regulates trophoblast proliferation and invasiveness through fibroblast growth factor 1 in selective intrauterine growth restriction. J Cell Mol Med 23:4422-4433 (2019). PubMed: 30993882
- Wang VE et al. Adaptive Resistance to Dual BRAF/MEK Inhibition in BRAF-Driven Tumors through Autocrine FGFR Pathway Activation. Clin Cancer Res 25:7202-7217 (2019). PubMed: 31515463
- Liu X et al. Long non-coding RNA Gas5 regulates proliferation and apoptosis in HCS-2/8 cells and growth plate chondrocytes by controlling FGF1 expression via miR-21 regulation. J Biomed Sci 25:18 (2018). PubMed: 29490650
- Li M et al. FGF1 Mediates Overnutrition-Induced Compensatory ß-Cell Differentiation. Diabetes 65:96-109 (2016). IF ; Zebrafish . PubMed: 26420862
- Jiang S et al. Novel insights into a treatment for aplastic anemia based on the advanced proliferation of bone marrow-derived mesenchymal stem cells induced by fibroblast growth factor 1. Mol Med Rep 12:7877-82 (2015). IF . PubMed: 26460236
- MacKenzie B et al. Attenuating endogenous Fgfr2b ligands during bleomycin-induced lung fibrosis does not compromise murine lung repair. Am J Physiol Lung Cell Mol Physiol 308:L1014-24 (2015). PubMed: 25820524
