Anti-FGF1 抗体 (ab9588)
Key features and details
- Rabbit polyclonal to FGF1
- Suitable for: Sandwich ELISA
- Reacts with: Rat, Human
- Isotype: IgG
製品の概要
-
製品名
Anti-FGF1 antibody
FGF1 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to FGF1 -
由来種
Rabbit -
アプリケーション
適用あり: Sandwich ELISAmore details -
種交差性
交差種: Rat, Human -
免疫原
Highly pure (>98%) recombinant FGF1 (human Fibroblast Growth Factor-acidic).
-
ポジティブ･コントロール
-
特記事項
This product is no longer batch tested in IHC, for an IHC validated antibody please see ab179455
製品の特性
-
製品の状態
Lyophilized:Reconstitute with 200µl of sterile water. -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
バッファー
pH: 7.40 -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
軽鎖の種類
unknown -
研究分野
関連製品
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
アプリケーション
Our Abpromise guarantee covers the use of ab9588 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| Sandwich ELISA | Use at an assay dependent concentration. To detect human FGF1 by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.25 – 1.0 μg/ml of ab83163 is required. This biotinylated polyclonal antibody, in conjunction with ab83163 as a capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant human FGF1. |
ターゲット情報
-
機能
The heparin-binding fibroblast growth factors play important roles in the regulation of cell survival, cell division, angiogenesis, cell differentiation and cell migration. They are potent mitogens in vitro. -
配列類似性
Belongs to the heparin-binding growth factors family. -
細胞内局在
Secreted. Cytoplasm. Cytoplasm > cell cortex. Lacks a cleavable signal sequence. Within the cytoplasm, it is transported to the cell membrane and then secreted by a non-classical pathway that requires Cu(2+) ions and S100A13. Secreted in a complex with SYT1. - Information by UniProt
-
参照データベース
- Entrez Gene: 2246 Human
- Entrez Gene: 25317 Rat
- Omim: 131220 Human
- SwissProt: P05230 Human
- SwissProt: P61149 Rat
- Unigene: 483635 Human
- Unigene: 88013 Rat
-
別名
- Acidic fibroblast growth factor antibody
- aFGF antibody
- Beta endothelial cell growth factor antibody
see all
画像
-
Sandwich ELISA - Anti-FGF1 antibody (ab9588)ab9588 detecting FGF-1 in sELISA
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
参考文献 (6)
ab9588 は 6 報の論文で使用されています。
- Xue YN et al. LncRNA TUG1 regulates FGF1 to enhance endothelial differentiation of adipose-derived stem cells by sponging miR-143. J Cell Biochem 120:19087-19097 (2019). PubMed: 31264280
- Li L et al. miRNA-210-3p regulates trophoblast proliferation and invasiveness through fibroblast growth factor 1 in selective intrauterine growth restriction. J Cell Mol Med 23:4422-4433 (2019). PubMed: 30993882
- Liu X et al. Long non-coding RNA Gas5 regulates proliferation and apoptosis in HCS-2/8 cells and growth plate chondrocytes by controlling FGF1 expression via miR-21 regulation. J Biomed Sci 25:18 (2018). PubMed: 29490650
- Li M et al. FGF1 Mediates Overnutrition-Induced Compensatory ß-Cell Differentiation. Diabetes 65:96-109 (2016). IF ; Zebrafish . PubMed: 26420862
- Jiang S et al. Novel insights into a treatment for aplastic anemia based on the advanced proliferation of bone marrow-derived mesenchymal stem cells induced by fibroblast growth factor 1. Mol Med Rep 12:7877-82 (2015). IF . PubMed: 26460236
- MacKenzie B et al. Attenuating endogenous Fgfr2b ligands during bleomycin-induced lung fibrosis does not compromise murine lung repair. Am J Physiol Lung Cell Mol Physiol 308:L1014-24 (2015). PubMed: 25820524
