Anti-FGF basic 抗体 [MC-GF1] (ab181)
製品の概要
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製品名
Anti-FGF basic antibody [MC-GF1]
FGF2 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [MC-GF1] to FGF basic -
由来種
Mouse -
特異性
GF1 recognises basic fibroblast growth factor (bFGF). -
アプリケーション
適用あり: ELISA, IHC-Frmore details -
種交差性
交差種: Mouse, Human, Pig -
免疫原
Recombinant full length protein (Human).
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.1% Sodium azide -
Concentration information loading...
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精製度
Protein G purified -
特記事項(精製)
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant. -
ポリ/モノ
モノクローナル -
クローン名
MC-GF1 -
ミエローマ
Sp2 -
アイソタイプ
IgG2a -
軽鎖の種類
unknown -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
Our Abpromise guarantee covers the use of ab181 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
アプリケーション | Abreviews | 特記事項 |
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ELISA | Use at an assay dependent concentration. | |
IHC-Fr | 1/250. PubMed: 17327226Fix with acetone. Not suitable for detecting bFGF bound to cell surface receptors. |
ターゲット情報
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機能
Plays an important role in the regulation of cell survival, cell division, angiogenesis, cell differentiation and cell migration. Functions as potent mitogen in vitro. Can induce angiogenesis (PubMed:23469107). -
組織特異性
Expressed in granulosa and cumulus cells. Expressed in hepatocellular carcinoma cells, but not in non-cancerous liver tissue. -
配列類似性
Belongs to the heparin-binding growth factors family. -
翻訳後修飾
Phosphorylation at Tyr-215 regulates FGF2 unconventional secretion.
Several N-termini starting at positions 94, 125, 126, 132, 143 and 162 have been identified by direct sequencing. -
細胞内局在
Secreted. Nucleus. Exported from cells by an endoplasmic reticulum (ER)/Golgi-independent mechanism. Unconventional secretion of FGF2 occurs by direct translocation across the plasma membrane. Binding of exogenous FGF2 to FGFR facilitates endocytosis followed by translocation of FGF2 across endosomal membrane into the cytosol. Nuclear import from the cytosol requires the classical nuclear import machinery, involving proteins KPNA1 and KPNB1, as well as CEP57. - Information by UniProt
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参照データベース
- Entrez Gene: 2247 Human
- Entrez Gene: 14173 Mouse
- Omim: 134920 Human
- SwissProt: P09038 Human
- SwissProt: P15655 Mouse
- Unigene: 284244 Human
- Unigene: 473689 Mouse
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別名
- Basic fibroblast growth factor antibody
- Basic fibroblast growth factor bFGF antibody
- BFGF antibody
see all
画像
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Immunohistochemistry (Frozen sections) - Anti-FGF basic antibody [MC-GF1] (ab181)Image from Morss AS et al., J Biol Chem. 2007 May 11;282(19):14635-44. Epub 2007 Feb 27. Fig 7.; doi: 10.1074/jbc.M608565200; May 11, 2007 The Journal of Biological Chemistry, 282, 14635-14644.ab181 staining FGF basic in normal (upper) and diabetic (lower) porcine aortic tissue by Immunohistochemistry (Frozen sections).
Tissue was fixed with acetone and air dried for 24 hours. Sections were blocked for endogenous peroxidase activity and incubated with primary antibody at 1/250 dilution. Staining was detected using DAB and counterstained with hematoxylin.
参考文献
This product has been referenced in:
- Miao H et al. Vascular Endothelial Growth Factor, Basic Fibroblast Growth Factor, and Pigment Epithelium-Derived Factor Expression in the Neovascular Iris in Retinal Diseases. J Ophthalmol 2018:8025951 (2018). Read more (PubMed: 29850214) »
- Liu B et al. Expressions of TGF-ß2, bFGF and ICAM-1 in lens epithelial cells of complicated cataract with silicone oil tamponade. Int J Ophthalmol 10:1034-1039 (2017). Read more (PubMed: 28730103) »