Anti-FGF basic 抗体 [MC-GF1] (ab181)

Reviews (2)Q&A (10)References (7)

製品の概要

  • 製品名

    Anti-FGF basic antibody [MC-GF1]
    FGF2 一次抗体 製品一覧

  • 製品の詳細

    Mouse monoclonal [MC-GF1] to FGF basic

  • 由来種

    Mouse

  • 特異性

    GF1 recognises basic fibroblast growth factor (bFGF).

  • アプリケーション

    適用あり: ELISA, IHC-Frmore details

  • 種交差性

    交差種: Mouse, Human, Pig

  • 免疫原

    Recombinant full length protein (Human).

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab181 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA Use at an assay dependent concentration.
IHC-Fr 1/250. PubMed: 17327226Fix with acetone. Not suitable for detecting bFGF bound to cell surface receptors.

ターゲット情報

  • 機能

    Plays an important role in the regulation of cell survival, cell division, angiogenesis, cell differentiation and cell migration. Functions as potent mitogen in vitro. Can induce angiogenesis (PubMed:23469107).

  • 組織特異性

    Expressed in granulosa and cumulus cells. Expressed in hepatocellular carcinoma cells, but not in non-cancerous liver tissue.

  • 配列類似性

    Belongs to the heparin-binding growth factors family.

  • 翻訳後修飾

    Phosphorylation at Tyr-215 regulates FGF2 unconventional secretion.
    Several N-termini starting at positions 94, 125, 126, 132, 143 and 162 have been identified by direct sequencing.

  • 細胞内局在

    Secreted. Nucleus. Exported from cells by an endoplasmic reticulum (ER)/Golgi-independent mechanism. Unconventional secretion of FGF2 occurs by direct translocation across the plasma membrane. Binding of exogenous FGF2 to FGFR facilitates endocytosis followed by translocation of FGF2 across endosomal membrane into the cytosol. Nuclear import from the cytosol requires the classical nuclear import machinery, involving proteins KPNA1 and KPNB1, as well as CEP57.
  • Information by UniProt

  • 参照データベース

    • 別名

      • Basic fibroblast growth factor antibody
      • Basic fibroblast growth factor bFGF antibody
      • BFGF antibody
      • FGF 2 antibody
      • FGF B antibody
      • FGF-2 antibody
      • Fgf2 antibody
      • FGF2 basic antibody
      • FGF2_HUMAN antibody
      • FGFB antibody
      • Fibroblast growth factor 2 (basic) antibody
      • Fibroblast growth factor 2 antibody
      • Fibroblast growth factor, basic antibody
      • HBGF 2 antibody
      • HBGF-2 antibody
      • HBGF2 antibody
      • HBGH 2 antibody
      • HBGH2 antibody
      • Heparin binding growth factor 2 precursor antibody
      • Heparin-binding growth factor 2 antibody
      • Prostatropin antibody
      see all

    画像

    • Immunohistochemistry (Frozen sections) - Anti-FGF basic antibody [MC-GF1] (ab181)
      Immunohistochemistry (Frozen sections) - Anti-FGF basic antibody [MC-GF1] (ab181)Image from Morss AS et al., J Biol Chem. 2007 May 11;282(19):14635-44. Epub 2007 Feb 27. Fig 7.; doi: 10.1074/jbc.M608565200; May 11, 2007 The Journal of Biological Chemistry, 282, 14635-14644.
      ab181 staining FGF basic in normal (upper) and diabetic (lower) porcine aortic tissue by Immunohistochemistry (Frozen sections).

      Tissue was fixed with acetone and air dried for 24 hours. Sections were blocked for endogenous peroxidase activity and incubated with primary antibody at 1/250 dilution. Staining was detected using DAB and counterstained with hematoxylin.

    プロトコール

    参考文献

    This product has been referenced in:

    • Miao H  et al. Vascular Endothelial Growth Factor, Basic Fibroblast Growth Factor, and Pigment Epithelium-Derived Factor Expression in the Neovascular Iris in Retinal Diseases. J Ophthalmol 2018:8025951 (2018). Read more (PubMed: 29850214) »
    • Liu B  et al. Expressions of TGF-ß2, bFGF and ICAM-1 in lens epithelial cells of complicated cataract with silicone oil tamponade. Int J Ophthalmol 10:1034-1039 (2017). Read more (PubMed: 28730103) »
    See all 7 Publications for this product

    ab181 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    レビューと Q&A

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    1-10 of 12 Abreviews or Q&A

    Western blot abreview for Anti-FGF2 antibody [MC-GF1]

     Average
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Pig Tissue lysate - whole (Stomach)
    Gel Running Conditions
    Reduced Denaturing (15%)
    Loading amount
    30 µg
    Specification
    Stomach
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Read More

    Abcam user community

    Verified customer

    投稿 Aug 28 2018

    Flow Cytometry abreview for Anti-FGF2 antibody [MC-GF1]

     Good
    Abreviews
    abreview image
    Application
    Flow Cytometry
    Sample
    Mouse Cell (Primary hepatocytes)
    Permeabilization
    Yes - Fluidigm Fix and Perm Buffer
    Gating Strategy
    Positive cells for APC
    Specification
    Primary hepatocytes
    Preparation
    Cell harvesting/tissue preparation method: Liver dissection, followed by mashing the liver to obtain hepatocytes
    Sample buffer: PBS + 10% Bovine Serum
    Fixation
    Formaaldehyde
    Read More

    Abcam user community

    Verified customer

    投稿 May 28 2016

    Question

    I checked your website but was not able to find the information I needed about Your anti-bFGF antibody AB181. Is it possible to give me more information about the pI and the sequence of this antibody?
    Thank you in advance!

    Read More
    Answer

    Thank you for your inquiry.
    The immunogen used to raise the anti-FGF basic antibody ab181 is the recombinant full length protein(human).
    Unfortunately, the epitope of this clone has not been mapped and the pI of our antibodiesare not determined.
    We will update the on-line datasheet for this product as soon as we receive additional data.

    We apologize for any inconvenience this may cause.

    Read More

    Question

    Hello, I do not have the order confirmation number but this was placed under PO# xxxx

    Read More
    Answer

    Thank you for your reply.

    I have searched this for this PO and the product's order history an cannot find any order using this PO or to Pfizer placed within the last two years. Was this product purchased from an outside distributor?

    I look forward to your reply. Please let me know if you have any questions or there are other ways that Abcam may help you meet your research goals.

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    Question

    Thanks for the information. That is really unfortunate, since that is the reason we purchased this particular antibody.

    Read More
    Answer

    Thank you for your reply.

    We are very sorry about this. We will happily replace or refund this product for you.

    I look forward to your reply on how you like to proceed. Please do not hesitate to contact us if you need any more advice or information.

    Use our products? Submit an Abreview. Earn rewards!

    https://www.abcam.com/abreviews

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    Question

    Customer kindly contacted us to inquire about the reference data used for the claim that this product is competitively displaced by heparin.

    Read More
    Answer

    Thank you for your patience as I contacted our lab regarding your inquiry.

    I had looked through data regarding this product and found no information to support the statement on the datasheet. These finding were passed onto the production lab who have confirmed that the details were entered in error. The statement is being removed and should be live on the website shortly. We do apologize for any inconveniences which this error made have created for you.

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Use our products? Submit an Abreview. Earn rewards!

    https://www.abcam.com/abreviews

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    Question

    Customer kindly contacted us to inquire about the reference data used for the claim that this product is is competitively displaced by heparin.

    Read More
    Answer

    Thank you very much for contacting us.

    I just wanted to let you know that I am still working on your inquiry. I have contacted the originator of this product for the information you requested, and I am awaiting a reply.

    I am very sorry for the delay. I will forward to you the information as soon as I receive it. Thank you for your patience.

    Read More

    Question

    Would like to test in IHC-P with pig sections.

    Read More
    Answer

    DISCOUNT CODE: ***
    Expiration date: November 6, 2012

    This code will give you 1 freeprimary antibodybefore the expiration date. To redeem this offer, please submit an Abreview forab181 and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. Please remember that submission of the Abreview is sufficient for the discount code to become active. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.

    The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

    Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

    The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

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    Question

    Dear,Some time ago I ordered different monoclonal anti-bFGF antibodies: Ab 181, Ab 17505, Ab 18629, Ab 18631, Ab 18634 and Ab 10419. I was wondering if you have any information about their binding to the bFGF, do they bind to the same epitopes or can I for example perform a sandwich-assay with two of these antibodies?

    Thanks in advance.

    Best regards,

    Read More
    Answer

    I have received further information from lab. Unfortunately lab has confirmed that because they do not have any tested data of these antibodies being used as pairs in sELISA and also due to non availability of epitope sequence they are unable to confirm the sue of these products in sELISA.

    I am sorry I am not very helpful this time. Should you have any other question please do not hesitate to contact me.

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    Question

    Dear,Some time ago I ordered different monoclonal anti-bFGF antibodies: Ab 181, Ab 17505, Ab 18629, Ab 18631, Ab 18634 and Ab 10419. I was wondering if you have any information about their binding to the bFGF, do they bind to the same epitopes or can I for example perform a sandwich-assay with two of these antibodies?
    Thanks in advance.
    Best regards,

    Read More
    Answer

    Thank you for your email.

    We unfortunately have not tested any of these antibodies in sandwich ELISA so we will not be able to confirm their use. Technically, sELISA application needs two antibodies (sELISA pair) which should be raised against different part of immunogen to avoid any interference between the two; the pair should be tested in sELISA.

    For these antibodies, also the immunogenic epitope sequence is not available yet so we can't recommend which ab should be used as detection or capture antibody. It seems, these antibodies are tested in indirect ELISA so we are happy to guarantee the iELISA application.

    To know more about the sELISA specificity I have sent an email to my colleagues for double checking if these can be used as sELISA pairs. I will send you the information soon.

    I can suggest trying these antibodies in sELISA however please note because all of these antibodies are monoclonal so either you will need to conjugate one of these antibodies or you will need an anti-mouse conjugated tertiary antibody. The positive thing, these antibodies have different isotypes so the anti mouse tertiary antibody you would need could be against a particular isotype e.g. IgG2a which should not cross react with IgG1.

    Alternatively we have conjugation kits available in case you are interested in conjugating one of these antibody with HRP or other conjugates. The catalogue numbers are:

    ab102892, ab102890, ab102891, ab102889 etc.

    https://www.abcam.com/index.html?pageconfig=resource&rid=13148

    I hope this information will be helpful. Should you have any other question please do not hesitate to contact me.

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