Anti-FGD4 抗体 (ab97785)
Key features and details
- Rabbit polyclonal to FGD4
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
製品の概要
-
製品名
Anti-FGD4 antibody -
製品の詳細
Rabbit polyclonal to FGD4 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-P, ICC/IFmore details -
種交差性
交差種: Human -
免疫原
Recombinant fragment, corresponding to a sequence within amino acids 49-297 of Human FGD4 (NP_640334).
-
ポジティブ・コントロール
- 293T, A431, H1299, HeLa, HepG2, MOLT4 and Raji whole cell lysates; HeLa cells; SG xenograft tissue.
-
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading...
-
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG
関連製品
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab97785の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
WB |
1/500 - 1/3000. Predicted molecular weight: 87 kDa.
|
|
IHC-P |
Use at an assay dependent concentration.
|
|
ICC/IF |
1/100 - 1/200.
|
特記事項 |
---|
WB
1/500 - 1/3000. Predicted molecular weight: 87 kDa. |
IHC-P
Use at an assay dependent concentration. |
ICC/IF
1/100 - 1/200. |
ターゲット情報
-
機能
Activates CDC42, a member of the Ras-like family of Rho-and Rac proteins, by exchanging bound GDP for free GTP. Plays a role in regulating the actin cytoskeleton and cell shape. Activates MAPK8. -
組織特異性
Expressed in different tissues, including brain, cerebellum, peripheral nerve, skeletal muscle, heart, uterus, placenta and testis. -
関連疾患
Charcot-Marie-Tooth disease 4H (CMT4H) [MIM:609311]: A recessive demyelinating form of Charcot-Marie-Tooth disease, a disorder of the peripheral nervous system, characterized by progressive weakness and atrophy, initially of the peroneal muscles and later of the distal muscles of the arms. Charcot-Marie-Tooth disease is classified in two main groups on the basis of electrophysiologic properties and histopathology: primary peripheral demyelinating neuropathies (designated CMT1 when they are dominantly inherited) and primary peripheral axonal neuropathies (CMT2). Demyelinating neuropathies are characterized by severely reduced nerve conduction velocities (less than 38 m/sec), segmental demyelination and remyelination with onion bulb formations on nerve biopsy, slowly progressive distal muscle atrophy and weakness, absent deep tendon reflexes, and hollow feet. By convention autosomal recessive forms of demyelinating Charcot-Marie-Tooth disease are designated CMT4. Note=The disease is caused by mutations affecting the gene represented in this entry. -
配列類似性
Contains 1 DH (DBL-homology) domain.
Contains 1 FYVE-type zinc finger.
Contains 2 PH domains. -
ドメイン
The part of the protein spanning the actin filament-binding domain together with the DH domain and the first PH domain is necessary and sufficient for microspike formation. Activation of MAPK8 requires the presence of all domains with the exception of the actin filament-binding domain. -
細胞内局在
Cytoplasm > cytoskeleton. Cell projection > filopodium. Concentrated in filopodia and poorly detected at lamellipodia. Binds along the sides of actin fibers. - Information by UniProt
-
参照データベース
- Entrez Gene: 121512 Human
- Omim: 611104 Human
- SwissProt: Q96M96 Human
- Unigene: 117835 Human
-
別名
- 9030023J02Rik antibody
- 9330209B17Rik antibody
- Actin filament binding protein frabin antibody
see all
画像
-
Anti-FGD4 antibody (ab97785) at 1/1000 dilution + MOLT4 whole cell lysate at 30 µg
Predicted band size: 87 kDa
7.5% SDS-PAGE. -
ab97785 at 1/200 dilution staining FGD4 in HeLa cells by Immunofluorescence, Paraformaldehyde fixed. Lower image is merged with DNA probe.
-
ab97785 at 1/100 dilution staining FGD4 in SG xenograft by Immunohistochemistry, Paraffin-embedded tissue.
プロトコール
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (4)
ab97785 は 4 報の論文で使用されています。
- Lin J et al. MiR-23a induced the activation of CDC42/PAK1 pathway and cell cycle arrest in human cov434 cells by targeting FGD4. J Ovarian Res 13:90 (2020). PubMed: 32772928
- Brooks AB et al. MYO6 is targeted bySalmonellavirulence effectors to trigger PI3-kinase signaling and pathogen invasion into host cells. Proc Natl Acad Sci U S A 114:3915-3920 (2017). PubMed: 28348208
- Cao M et al. Mechanisms of Impaired Neutrophil Migration by MicroRNAs in Myelodysplastic Syndromes. J Immunol 198:1887-1899 (2017). PubMed: 28130497
- Ottman R et al. MicroRNA expressions associated with progression of prostate cancer cells to antiandrogen therapy resistance. Mol Cancer 13:1 (2014). WB ; Human . PubMed: 24387052