製品の概要

  • 製品名

    FFN511, Fluorescent substrate for VMAT2
  • 製品の詳細

    First novel fluorescent false neurotransmitter. Enables optical imaging of presynaptic terminal activity.
  • 別名

    • Fluorescent False Neurotransmitter 511
  • 生理活性の詳細

    First novel fluorescent false neurotransmitter. Enables optical imaging of presynaptic terminal activity.
    Inhibits 5-HT binding to VMAT2 (IC50 = 1 µM (comparable to dopamine itself)). Released via exocytosis in the striatum in mouse acute slice preparation. Labels dopamine and other presynaptic terminals in the striatum.
    Compatible with GFP tags and other optical probes. Sufficiently bright, photostable and suitable for two-photon fluorescence microscopy.
    View the technique online

    For highly selective labelling of dopaminergic presynaptic termainals FFN102 (ab120866) is recommended.
  • 精製度

    > 98%
  • CAS 番号

    1004548-96-2
  • 構造式

    Chemical Structure

製品の特性

参考文献

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レビューと Q&A

1-3 of 3 Abreviews or Q&A

Answer

Thank you for contacting us and your interest in our products.

The information we have in relation to the emission and excitation of FFN511 is from the following reference:

Gubernator NG et al. Fluorescent false neurotransmitters visualize dopamine release from individual presynaptic terminals. Science 324:1441-4 (2009). (PubMed: 19423778)

Although the emission and excitation maxima for FFN511 are 406 nm and 501 nm respectively (in pH7 buffer), the fluorescence measurements taken by Gubernator et al. was using two-photon excitation microscopy. The excitation was performed using a multiphoton laser scanning microscope with a Titanium-sapphire laser with excitation of 760 nm applied and the emission measured using 480-520 nm band pass filter.

I have attached the journal article and the supplementary material for your information.

In order to receive a quote for the products I will need to ask our official distributor to Israel to help. I have CC'd them into this email and they should be in contact with a quote.

I hope this information has been of help. If you have any further questions, please do not hesitate to contact us again.

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Answer

Thank you for your inquiry.

We do ot have any information about the pKa value of FFN511. The available literature also does not seem to mention the pKa value of FFN511.

In relation to the pH range, the only mention is in the supplemental information

Gubernator NG et al. Fluorescent false neurotransmitters visualize dopamine release from individual presynaptic terminals. Science 324:1441-4 (2009).(PubMed: 19423778)



,is that it was used at pH 7.4. I would assume this is due to it being a physiologically relevant pH.

I am sorry I did not have more information for you on this occasion and hope this was nevertheless helpful.

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Question
Answer

As we do not currently undertake our own research I can offer you advice based on information available in a recent paper by Gubernator et al., 2009. I can confirm that excitation of FFN551 occurs at 760 nm, with optimal fluorescence of striatal terminals seen through a band pass filter (emission 480-520 nm). I have attached a copy of the paper for your information. I have also attached a copy of the supporting material where there is more detailed information regarding the
photo physical properties of FFN511.
More information can be found in the links on our website (see Asc-331).
I hope you find this information helpful. If you have any further questions please do not hesitate to contact us.

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