Anti-fetal hemoglobin 抗体 [EPR9708(B)] (ab137096)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR9708(B)] to fetal hemoglobin
- Suitable for: WB
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-fetal hemoglobin antibody [EPR9708(B)]
fetal hemoglobin 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR9708(B)] to fetal hemoglobin -
由来種
Rabbit -
特異性
This antibody recognises Hemoglobin gamma-1/2 chain.
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アプリケーション
適用あり: WBmore details
適用なし: IHC-P -
種交差性
交差種: Human -
免疫原
Synthetic peptide within Human fetal hemoglobin. The exact sequence is proprietary.
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ポジティブ・コントロール
- WB: Human red blood cell and K562 cell lysates.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20ºC. -
バッファー
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR9708(B) -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab137096の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/1000 - 1/10000. Predicted molecular weight: 16 kDa.
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特記事項 |
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WB
1/1000 - 1/10000. Predicted molecular weight: 16 kDa. |
ターゲット情報
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関連性
The gamma globin genes (HBG1 and HBG2) are normally expressed in the fetal liver, spleen and bone marrow. Two gamma chains together with two alpha chains constitute fetal hemoglobin (HbF) which is normally replaced by adult hemoglobin (HbA) at birth. In some beta-thalassemias and related conditions, gamma chain production continues into adulthood. The two types of gamma chains differ at residue 136 where glycine is found in the G-gamma product (HBG2) and alanine is found in the A-gamma product (HBG1). The former is predominant at birth. The order of the genes in the beta-globin cluster is: 5'-epsilon -- gamma-G -- gamma-A -- delta -- beta--3' -
細胞内局在
Cytoplasmic -
参照データベース
- Entrez Gene: 3047 Human
- Entrez Gene: 3048 Human
- Omim: 142200 Human
- Omim: 142250 Human
- SwissProt: P69891 Human
- SwissProt: P69892 Human
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別名
- A gamma globin antibody
- G gamma globin antibody
- Gamma 1 globin antibody
see all
画像
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Anti-fetal hemoglobin antibody [EPR9708(B)] (ab137096) at 1/2000 dilution (Purified) + K-562 (Human chronic myelogenous leukemia lymphoblast) whole cell lysates at 15 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 16 kDa
Observed band size: 13 kDa why is the actual band size different from the predicted? -
All lanes : Anti-fetal hemoglobin antibody [EPR9708(B)] (ab137096) at 1/2000 dilution (Unpurified)
Lane 1 : Human red blood cell
Lane 2 : K562 (human lymphoblast from chronic myelogenous leukemia) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/1000 dilution
Predicted band size: 16 kDaBlocking and Diluting Buffer:5% NFDM/TBST
Exposure time:3 min
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (6)
ab137096 は 6 報の論文で使用されています。
- Jin T et al. Transmembrane Protein ANTXR1 Regulates γ-Globin Expression by Targeting the Wnt/β-Catenin Signaling Pathway. J Immunol Res 2022:8440422 (2022). PubMed: 35942209
- Li X et al. Transcriptional silencing of fetal hemoglobin expression by NonO. Nucleic Acids Res 49:9711-9723 (2021). PubMed: 34379783
- Boontanrart MY et al. ATF4 Regulates MYB to Increase ?-Globin in Response to Loss of ß-Globin. Cell Rep 32:107993 (2020). PubMed: 32755585
- Sun KT et al. Reciprocal regulation of ?-globin expression by exo-miRNAs: Relevance to ?-globin silencing in ß-thalassemia major. Sci Rep 7:202 (2017). WB . PubMed: 28303002
- Li J et al. SOX6 Downregulation Induces?-Globin in Humanß-Thalassemia Major Erythroid Cells. Biomed Res Int 2017:9496058 (2017). PubMed: 29333458
- Niu X et al. Combining Single Strand Oligodeoxynucleotides and CRISPR/Cas9 to Correct Gene Mutations in ß-Thalassemia-induced Pluripotent Stem Cells. J Biol Chem 291:16576-85 (2016). WB ; Human . PubMed: 27288406