Anti-fetal hemoglobin 抗体 (ab19364)
Key features and details
- Sheep polyclonal to fetal hemoglobin
- Suitable for: ELISA, IHC-P
- Reacts with: Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-fetal hemoglobin antibody
fetal hemoglobin 一次抗体 製品一覧 -
製品の詳細
Sheep polyclonal to fetal hemoglobin -
由来種
Sheep -
特異性
By immunoelectrophoresis and ELISA this antibody reacts specifically with human hemoglobin F. No antibody was detected against hemoglobin A1, A2, S or human serum proteins. -
アプリケーション
適用あり: ELISA, IHC-Pmore details -
種交差性
交差種: Human
交差が予測される動物種: Chimpanzee, Rhesus monkey, Gorilla -
免疫原
Full length protein corresponding to Human fetal hemoglobin. Sheep were immunized with purified human hemoglobin F of fetal cord blood cell origin.
Database link: P69891 -
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 6.8
Preservative: 0.1% Sodium azide
Constituent: PBS -
Concentration information loading...
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精製度
Immunogen affinity purified -
特記事項(精製)
The antibody was isolated by affinity chromato-graphy using antigen coupled to agarose beads. Antibody concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab19364の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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ELISA |
Use at an assay dependent concentration.
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IHC-P |
Use at an assay dependent concentration. PubMed: 21378008
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特記事項 |
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ELISA
Use at an assay dependent concentration. |
IHC-P
Use at an assay dependent concentration. PubMed: 21378008 |
ターゲット情報
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関連性
The gamma globin genes (HBG1 and HBG2) are normally expressed in the fetal liver, spleen and bone marrow. Two gamma chains together with two alpha chains constitute fetal hemoglobin (HbF) which is normally replaced by adult hemoglobin (HbA) at birth. In some beta-thalassemias and related conditions, gamma chain production continues into adulthood. The two types of gamma chains differ at residue 136 where glycine is found in the G-gamma product (HBG2) and alanine is found in the A-gamma product (HBG1). The former is predominant at birth. The order of the genes in the beta-globin cluster is: 5'-epsilon -- gamma-G -- gamma-A -- delta -- beta--3' -
細胞内局在
Cytoplasmic -
参照データベース
- Entrez Gene: 3047 Human
- Entrez Gene: 3048 Human
- Omim: 142200 Human
- Omim: 142250 Human
- SwissProt: P69891 Human
- SwissProt: P69892 Human
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別名
- A gamma globin antibody
- G gamma globin antibody
- Gamma 1 globin antibody
see all
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (5)
ab19364 は 5 報の論文で使用されています。
- Guo L et al. Oridonin enhances ?-globin expression in erythroid precursors from patients with ß-thalassemia via activation of p38 MAPK signaling. Mol Med Rep 21:909-917 (2020). PubMed: 31789406
- Wolk M & Martin JE Fetal haemopoiesis marking low-grade urinary bladder cancer. Br J Cancer 107:477-81 (2012). PubMed: 22735903
- Wolk M & Martin JE Fetal haemoglobin (HbF) as an immunohistochemical tumour marker in bone marrow and spleen. J Clin Pathol 64:645-7 (2011). IHC-P ; Human . PubMed: 21378008
- Yang M et al. Effects of Astragalus polysaccharide on the erythroid lineage and microarray analysis in K562 cells. J Ethnopharmacol 127:242-50 (2010). WB . PubMed: 19922785
- Zhang X et al. In vitro maturation of erythroid progenitors from human umbilical cord blood and patterns of globin gene expression: serum from different developmental stage plays important roles in liquid culture. Biochem Biophys Res Commun 336:42-8 (2005). PubMed: 16125140