Fenofibrate, PPAR-alpha agonist (ab120832)
Key features and details
- Potent, selective PPAR-α agonist
- CAS Number: 49562-28-9
- Purity: > 99%
- Soluble in DMSO to 100 mM and in ethanol to 100 mM
- Form / State: Solid
- Source: Synthetic
製品の概要
-
製品名
Fenofibrate, PPAR-alpha agonist -
製品の詳細
Potent, selective PPAR-α agonist -
生理活性の詳細
Potent and selective PPAR-α agonist (EC50 values are 18 and 30 μM at mouse and human receptors, respectively). Affinity is 10-fold less at PPAR-γ (EC50 values are 250 and 300 μM at mouse and human receptors, respectively). Hypolipidemic agent.
-
精製度
> 99% -
CAS 番号
49562-28-9 -
構造式
製品の特性
-
体系名
2-[4-(4-Chlorobenzoyl)phenoxy]-2-methylpropanoic acid isopropyl ester -
分子量
360.84 -
分子式
C20H21ClO4 -
保存方法
Store at Room Temperature. The product can be stored for up to 12 months. -
溶解性
Soluble in DMSO to 100 mM and in ethanol to 100 mM -
使用に関する注意
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
-
由来
Synthetic
-
研究分野
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab120832の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| Functional Studies |
Use at an assay dependent concentration.
|
| 特記事項 |
|---|
|
Functional Studies
Use at an assay dependent concentration. |
画像
-
Chemical Structure - Fenofibrate, PPAR-alpha agonist (ab120832)2D chemical structure image of ab120832, Fenofibrate, PPAR-alpha agonist
-
Functional Studies - Fenofibrate, PPAR-alpha agonist (ab120832)HL-60 cells were incubated at 37°C for 24h with vehicle control (0 µM) and different concentrations of fenofibrate (ab120832). Increased expression of ACADM in HL-60 cells correlates with an increase in fenofibrate concentration, as described in literature.
Whole cell lysates were prepared with RIPA buffer (containing protease inhibitors and sodium orthovanadate), 10 µg of each were loaded on the gel and the WB was run under reducing conditions. After transfer the membrane was blocked for an hour using 5% BSA before being incubated with ab110296 at 1 µg/ml and ab9484 at 1 µg/ml overnight at 4°C. Antibody binding was detected using an anti-mouse antibody conjugated to HRP (ab97040) at 1/10000 dilution and visualised using ECL development solution.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
参考文献 (2)
ab120832 は 2 報の論文で使用されています。
- Takahashi H et al. TGF-ß2 is an exercise-induced adipokine that regulates glucose and fatty acid metabolism. Nat Metab 1:291-303 (2019). PubMed: 31032475
- Wang Z et al. Naoxintong Retards Atherosclerosis by Inhibiting Foam Cell Formation Through Activating Ppara Pathway. Curr Mol Med 18:698-710 (2018). PubMed: 30734676