製品名Fenofibrate, PPAR-alpha agonist
製品の詳細Potent, selective PPAR-α agonist
生理活性の詳細Potent and selective PPAR-α agonist (EC50 values are 18 and 30 μM at mouse and human receptors, respectively). Affinity is 10-fold less at PPAR-γ (EC50 values are 250 and 300 μM at mouse and human receptors, respectively). Hypolipidemic agent.
体系名2-[4-(4-Chlorobenzoyl)phenoxy]-2-methylpropanoic acid isopropyl ester
保存方法Store at Room Temperature. The product can be stored for up to 12 months.
溶解性Soluble in DMSO to 100 mM and in ethanol to 100 mM
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
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Our Abpromise guarantee covers the use of ab120832 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Functional Studies||Use at an assay dependent concentration.|
HL-60 cells were incubated at 37°C for 24h with vehicle control (0 µM) and different concentrations of fenofibrate (ab120832). Increased expression of ACADM in HL-60 cells correlates with an increase in fenofibrate concentration, as described in literature.
Whole cell lysates were prepared with RIPA buffer (containing protease inhibitors and sodium orthovanadate), 10 µg of each were loaded on the gel and the WB was run under reducing conditions. After transfer the membrane was blocked for an hour using 5% BSA before being incubated with ab110296 at 1 µg/ml and ab9484 at 1 µg/ml overnight at 4°C. Antibody binding was detected using an anti-mouse antibody conjugated to HRP (ab97040) at 1/10000 dilution and visualised using ECL development solution.
This product has been referenced in:
- Balakumar P et al. Pleiotropic actions of fenofibrate on the heart. Pharmacol Res 63:8-12 (2011). Read more (PubMed: 21093591) »
- Mandard S et al. The direct peroxisome proliferator-activated receptor target fasting-induced adipose factor (FIAF/PGAR/ANGPTL4) is present in blood plasma as a truncated protein that is increased by fenofibrate treatment. J Biol Chem 279:34411-20 (2004). Read more (PubMed: 15190076) »
- Willson TM et al. The PPARs: from orphan receptors to drug discovery. J Med Chem 43:527-50 (2000). Read more (PubMed: 10691680) »