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リコンビナントRabMAb

Anti-FEN1 抗体 [EPR4460(2)] (ab109132)

Submit a review Submit a question References (7)
Western blot - Anti-FEN1 antibody [EPR4460(2)] (ab109132)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FEN1 antibody [EPR4460(2)] (ab109132)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FEN1 antibody [EPR4460(2)] (ab109132)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FEN1 antibody [EPR4460(2)] (ab109132)
  • Anti-FEN1 antibody [EPR4460(2)] (ab109132)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR4460(2)] to FEN1
  • Suitable for: WB, IHC-P
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

製品の概要

  • 製品名

    Anti-FEN1 antibody [EPR4460(2)]
    FEN1 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR4460(2)] to FEN1

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: WB, IHC-Pmore details
    適用なし: Flow Cyt,ICC or IP

  • 種交差性

    交差種: Human
    交差が予測される動物種: Mouse, Rat

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
    (Peptide available as ab95382)

  • ポジティブ･コントロール

    • Jurkat, HeLa, Y79, and Daudi cell lysates, Human testis, Human tonsil, and Human urothelial carcinoma tissues

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.

  • バッファー

    pH: 7.20
    Preservative: 0.05% Sodium azide
    Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant
  • Concentration information loading...

  • 精製度

    Protein A purified

  • ポリ/モノ

    モノクローナル

  • クローン名

    EPR4460(2)

  • アイソタイプ

    IgG

  • 研究分野

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab109132の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB
1/1000 - 1/10000. Detects a band of approximately 42 kDa (predicted molecular weight: 42 kDa).Can be blocked with Recombinant Human FEN1 protein (ab95382).
IHC-P
1/500 - 1/750. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
特記事項
WB
1/1000 - 1/10000. Detects a band of approximately 42 kDa (predicted molecular weight: 42 kDa).Can be blocked with Recombinant Human FEN1 protein (ab95382).
IHC-P
1/500 - 1/750. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
追加情報
Is unsuitable for Flow Cyt,ICC or IP.

ターゲット情報

  • 機能

    Structure-specific nuclease with 5'-flap endonuclease and 5'-3' exonuclease activities involved in DNA replication and repair. During DNA replication, cleaves the 5'-overhanging flap structure that is generated by displacement synthesis when DNA polymerase encounters the 5'-end of a downstream Okazaki fragment. It enters the flap from the 5'-end and then tracks to cleave the flap base, leaving a nick for ligation. Also involved in the long patch base excision repair (LP-BER) pathway, by cleaving within the apurinic/apyrimidinic (AP) site-terminated flap. Acts as a genome stabilization factor that prevents flaps from equilibrating into structurs that lead to duplications and deletions. Also possesses 5'-3' exonuclease activity on nicked or gapped double-stranded DNA, and exhibits RNase H activity. Also involved in replication and repair of rDNA and in repairing mitochondrial DNA.

  • 配列類似性

    Belongs to the XPG/RAD2 endonuclease family. FEN1 subfamily.

  • 翻訳後修飾

    Acetylated by EP300. Acetylation inhibits both endonuclease and exonuclease activity. Acetylation also reduces DNA-binding activity but does not affect interaction with PCNA or EP300.
    Phosphorylation upon DNA damage induces relocalization to the nuclear plasma. Phosphorylation at Ser-187 by CDK2 occurs during late S-phase and results in dissociation from PCNA.
    Methylation at Arg-192 by PRMT5 impedes Ser-187 phosphorylation and increases interaction with PCNA.

  • 細胞内局在

    Nucleus > nucleolus. Nucleus > nucleoplasm. Mitochondrion. Resides mostly in the nucleoli and relocalizes to the nucleoplasm upon DNA damage.
  • Information by UniProt

  • 参照データベース

    see all

  • 別名

    • DNase IV antibody
    • FEN-1 antibody
    • FEN1 antibody
    • FEN1_HUMAN antibody
    • Flap endonuclease 1 antibody
    • Flap structure specific endonuclease 1 antibody
    • Flap structure-specific endonuclease 1 antibody
    • hFEN-1 antibody
    • hFEN1 antibody
    • Maturation factor 1 antibody
    • MF1 antibody
    • Rad2 antibody
    see all

画像

  • Western blot - Anti-FEN1 antibody [EPR4460(2)] (ab109132)
    Western blot - Anti-FEN1 antibody [EPR4460(2)] (ab109132)
    All lanes : Anti-FEN1 antibody [EPR4460(2)] (ab109132) at 1/1000 dilution

    Lane 1 : Jurkat cell lysate
    Lane 2 : HeLa cell lysate
    Lane 3 : Y79 cell lysate
    Lane 4 : Daudi cell lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 42 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FEN1 antibody [EPR4460(2)] (ab109132)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FEN1 antibody [EPR4460(2)] (ab109132)

    Immunohistochemical analysis of paraffin-embedded Human testis tissue using ab109132 at a dilution of 1/500.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FEN1 antibody [EPR4460(2)] (ab109132)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FEN1 antibody [EPR4460(2)] (ab109132)

    Immunohistochemical analysis of paraffin-embedded Human tonsil tissue using ab109132 at a dilution of 1/500.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FEN1 antibody [EPR4460(2)] (ab109132)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FEN1 antibody [EPR4460(2)] (ab109132)

    Immunohistochemical analysis of paraffin-embedded Human urothelial carcinoma tissue using ab109132 at a dilution of 1/500.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Anti-FEN1 antibody [EPR4460(2)] (ab109132)
    Anti-FEN1 antibody [EPR4460(2)] (ab109132)

参考文献 (7)

ab109132 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab109132 は 7 報の論文で使用されています。

  • Huang W  et al. Jianpi-yangwei decoction inhibits DNA damage repair in the drug resistance of gastric cancer by reducing FEN1 expression. BMC Complement Med Ther 20:196 (2020). PubMed: 32586310
  • Pu J  et al. IGF2BP2 Promotes Liver Cancer Growth Through an m6A-FEN1-Dependent Mechanism. Front Oncol 10:578816 (2020). PubMed: 33224879
  • Guo Y & Luo S miR-140-5p inhibits cervical cancer cell phenotypes via downregulating FEN1 to halt the cell cycle. Mol Med Rep 22:4919-4930 (2020). PubMed: 33173950
  • Isohookana J  et al. Proteins of the retinoblastoma pathway, FEN1 and MGMT are novel potential prognostic biomarkers in pancreatic adenocarcinoma. Pathol Res Pract 214:840-847 (2018). IHC-P ; Human . PubMed: 29735403
  • Ma S  et al. LncRNA BANCR promotes tumorigenesis and enhances adriamycin resistance in colorectal cancer. Aging (Albany NY) 10:2062-2078 (2018). PubMed: 30144787
  • Ward TA  et al. Small molecule inhibitors uncover synthetic genetic interactions of human flap endonuclease 1 (FEN1) with DNA damage response genes. PLoS One 12:e0179278 (2017). WB ; Human . PubMed: 28628639
  • Exell JC  et al. Cellularly active N-hydroxyurea FEN1 inhibitors block substrate entry to the active site. Nat Chem Biol 12:815-21 (2016). WB ; Human . PubMed: 27526030

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