Application
Western blot
Sample
Xenopus laevis Cell lysate - nuclear (XTC)
Gel Running Conditions
Reduced Denaturing (5-15)
Loading amount
3000 cells
Specification
XTC
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: TBS
Dilution
1/5000
Secondary antibody
Dilution
1/10000
Name
Non-Abcam antibody was used: Pierce/Fisher
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL
Negative control
XTC cytosol
Exposure
5 minute(s) and 0 second(s)
Bands
Specific: 47 kDa
Positive control
Hela whole cell lysate, 47kDa band was detected
Additional data
Additional Notes
This antibody is very impressive for humans, not for frogs though
Western blot lanes:
1. HeLa whole cell lysate
2. XTC whole cell lysate
3. XTC nuclei
4. XTC cytosol
Bands from bottom to top H2B, Fen1, Tubulin, Smc3
Abcam response
Thank you for participating in our AbTrial program for untested species or applications. We appreciate the feedback!
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Dawn Bender
Verified customer
投稿 Oct 16 2017