Anti-FEN1 抗体 (ab17994)
Key features and details
- Rabbit polyclonal to FEN1
- Suitable for: IP, WB, IHC-P
- Reacts with: Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-FEN1 antibody
FEN1 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to FEN1 -
由来種
Rabbit -
アプリケーション
適用あり: IP, WB, IHC-Pmore details -
種交差性
交差種: Human
交差が予測される動物種: Sheep, Horse, Cow, Pig, Chimpanzee, Gorilla, Chinese hamster, Orangutan, Bat
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免疫原
Synthetic peptide representing human Flap Structure-specific Endonuclease I encoded within exon 2 (LocusLink ID 2237). The peptide is derived from a portion of FEN1 that begins a few residues downstream from the I-domain as defined in Swiss-Prot entry P39748.
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ポジティブ･コントロール
- IP: HeLa whole cell lysate; WB: HeLa and HEK293T cells; IHC: human testicular seminoma tissue.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7
Preservative: 0.1% Sodium azide
Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab17994の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IP |
Use at 2-10 µg/mg of lysate.
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| WB | (1) |
1/2000 - 1/10000. Detects a band of approximately 47 kDa (predicted molecular weight: 42 kDa).
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| IHC-P |
1/200 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| 特記事項 |
|---|
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IP
Use at 2-10 µg/mg of lysate. |
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WB
1/2000 - 1/10000. Detects a band of approximately 47 kDa (predicted molecular weight: 42 kDa). |
|
IHC-P
1/200 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ターゲット情報
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機能
Structure-specific nuclease with 5'-flap endonuclease and 5'-3' exonuclease activities involved in DNA replication and repair. During DNA replication, cleaves the 5'-overhanging flap structure that is generated by displacement synthesis when DNA polymerase encounters the 5'-end of a downstream Okazaki fragment. It enters the flap from the 5'-end and then tracks to cleave the flap base, leaving a nick for ligation. Also involved in the long patch base excision repair (LP-BER) pathway, by cleaving within the apurinic/apyrimidinic (AP) site-terminated flap. Acts as a genome stabilization factor that prevents flaps from equilibrating into structurs that lead to duplications and deletions. Also possesses 5'-3' exonuclease activity on nicked or gapped double-stranded DNA, and exhibits RNase H activity. Also involved in replication and repair of rDNA and in repairing mitochondrial DNA. -
配列類似性
Belongs to the XPG/RAD2 endonuclease family. FEN1 subfamily. -
翻訳後修飾
Acetylated by EP300. Acetylation inhibits both endonuclease and exonuclease activity. Acetylation also reduces DNA-binding activity but does not affect interaction with PCNA or EP300.
Phosphorylation upon DNA damage induces relocalization to the nuclear plasma. Phosphorylation at Ser-187 by CDK2 occurs during late S-phase and results in dissociation from PCNA.
Methylation at Arg-192 by PRMT5 impedes Ser-187 phosphorylation and increases interaction with PCNA. -
細胞内局在
Nucleus > nucleolus. Nucleus > nucleoplasm. Mitochondrion. Resides mostly in the nucleoli and relocalizes to the nucleoplasm upon DNA damage. - Information by UniProt
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参照データベース
- Entrez Gene: 616242 Cow
- Entrez Gene: 2237 Human
- Omim: 600393 Human
- SwissProt: Q58DH8 Cow
- SwissProt: P39748 Human
- Unigene: 409065 Human
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別名
- DNase IV antibody
- FEN-1 antibody
- FEN1 antibody
see all
画像
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Immunoprecipitation - Anti-FEN1 antibody (ab17994)
FEN1 was immunoprecipitated from HeLa whole cell lysate (1 mg per IP reaction, 20% loaded) with ab17994 at 6 µg per reaction. Western blot was performed on the immunoprecipitate using ab17994 at 1 µg/mL.
Lane 1: ab17994 IP in HeLa whole cell lysate.
Lane 2: ab17994 IP in HeLa whole cell lysate.
Lane 3: Control IgG in HeLa whole cell lysate.
Detection: Chemiluminescence with an exposure time of 30 seconds
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Western blot - Anti-FEN1 antibody (ab17994)All lanes : Anti-FEN1 antibody (ab17994) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate
Lane 2 : HEK293T whole cell lysate
Predicted band size: 42 kDa
Exposure time: 30 seconds -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FEN1 antibody (ab17994)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human testicular seminoma tissue labelling FEN1 with ab179944 at 1/1000 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab17994 は 1 報の論文で使用されています。
- Dong S et al. miR-193b Increases the Chemosensitivity of Osteosarcoma Cells by Promoting FEN1-Mediated Autophagy. Onco Targets Ther 12:10089-10098 (2019). PubMed: 31819503