Anti-FE65 抗体 (ab5668)
Key features and details
- Rabbit polyclonal to FE65
- Suitable for: ICC/IF, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
製品の概要
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製品名
Anti-FE65 antibody
FE65 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to FE65 -
由来種
Rabbit -
特異性
This antibody does not detect endogenous levels of Fe65. -
アプリケーション
適用あり: ICC/IF, WBmore details -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Synthetic peptide corresponding to Mouse FE65 aa 352-369.
Sequence:PQEEEKLSQRNANPGIKC
(Peptide available asab5892) -
ポジティブ・コントロール
- ICC/IF: SH-SY5Y (Human neuroblastoma cell line from bone marrow). WB: U-87 MG; SH-SY5Y; NTERA-2; U-2 OS; A549; Mouse Brain; PC-12
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.05% Sodium azide
Constituents: 99% PBS, 0.1% BSA -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab5668の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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ICC/IF |
Use a concentration of 2 µg/ml.
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WB |
Use a concentration of 0.2 µg/ml. Detects a band of approximately 97 kDa.
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特記事項 |
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ICC/IF
Use a concentration of 2 µg/ml. |
WB
Use a concentration of 0.2 µg/ml. Detects a band of approximately 97 kDa. |
ターゲット情報
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機能
Transcription coregulator that can have both coactivator and corepressor functions. Adapter protein that forms a transcriptionally active complex with the gamma-secretase-derived amyloid precursor protein (APP) intracellular domain. Plays a central role in the response to DNA damage by translocating to the nucleus and inducing apoptosis. May act by specifically recognizing and binding histone H2AX phosphorylated on 'Tyr-142' (H2AXY142ph) at double-strand breaks (DSBs), recruiting other pro-apoptosis factors such as MAPK8/JNK1. Required for histone H4 acetylation at double-strand breaks (DSBs). Its ability to specifically bind modified histones and chromatin modifying enzymes such as KAT5/TIP60, probably explains its trancription activation activity. Function in association with TSHZ3, SET and HDAC factors as a transcriptional repressor, that inhibits the expression of CASP4. Associates with chromatin in a region surrounding the CASP4 transcriptional start site(s). -
組織特異性
Highly expressed in brain; strongly reduced in post-mortem elderly subjects with Alzheimer disease. -
配列類似性
Contains 2 PID domains.
Contains 1 WW domain. -
翻訳後修飾
Phosphorylated following nuclear translocation. Phosphorylation at Tyr-546 enhances the transcription activation activity and reduces the affinity with RASD1/DEXRAS1. -
細胞内局在
Cell membrane. Cytoplasm. Nucleus. Cell projection > growth cone. Colocalizes with TSHZ3 in axonal growth cone (By similarity). In normal conditions, it mainly localizes to the cytoplasm, while a small fraction is tethered to the cell membrane via its interaction with APP. Following exposure to DNA damaging agents, it is released from cell membrane and translocates to the nucleus. Nuclear translocation is under the regulation of APP. Colocalizes with TSHZ3 in the nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 322 Human
- Entrez Gene: 11785 Mouse
- Entrez Gene: 29722 Rat
- Omim: 602709 Human
- SwissProt: O00213 Human
- SwissProt: Q9QXJ1 Mouse
- SwissProt: P46933 Rat
- Unigene: 372840 Human
see all -
別名
- Adaptor protein FE65a2 antibody
- Amyloid beta (A4) precursor protein binding family B member 1 antibody
- Amyloid beta A4 precursor protein binding family B antibody
see all
画像
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All lanes : Anti-FE65 antibody (ab5668) at 2 µg/ml
Lane 1 : U-87 MG (Human glioblastoma-astrocytoma epithelial cell line) whole cell lysate with blocking with 5 % skimmed milk
Lane 2 : SH-SY5Y (Human neuroblastoma cell line from bone marrow) whole cell lysate with blocking with 5 % skimmed milk
Lane 3 : NTERA-2 (Human malignant pluripotent embryonic carcinoma cell line) whole cell lysate with blocking with 5 % skimmed milk
Lane 4 : U-2 OS (Human bone osteosarcoma epithelial cell line) whole cell lysate with blocking with 5 % skimmed milk
Lane 5 : A549 (Human lung carcinoma cell line) whole cell lysate with blocking with 5 % skimmed milk
Lane 6 : Mouse Brain with blocking with 5 % skimmed milk
Lane 7 : PC-12 (Rat adrenal gland pheochromocytoma cell line) whole cell lysate with blocking with 5 % skimmed milk
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP conjugated Goat anti-Rabbit IgG at 1/2500 dilution
Additional bands at: ~77 kDa. We are unsure as to the identity of these extra bands.Detection: chemiluminescence
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Western blot of Fe65-affinity pulldown materials from mouse brain lysate using ab5668. Lane 1 is an immunopurified sample from a full-length Fe65 Knockout mouse. Lane 2 is an immunopurified sample from an Fe65 wild type mouse.
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Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton™ X-100 permeabilized, 70% confluent log phase SH-SY5Y (Human neuroblastoma cell line from bone marrow) cells, blocked with 1% BSA for 1 hour at room temperature. Labelling Fe65 with ab5668 at 2 µg/mL and incubated for 3 hours at room temperature and then labelled with Goat anti-Rabbit IgG secondary antibody, Alexa Fluor® 488 conjugate at a dilution of 1/2000 for 45 minutes at room temperature (Green). Nuclei were stained with SlowFade® Gold Antifade Mountant with DAPI (Blue). F-actin was stained with Alexa Fluor® 555 Rhodamine Phalloidin at a dilution of 1/300 (Red). Panel e shows the no primary antibody control. The images were captured at 60X magnification.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab5668 は 1 報の論文で使用されています。
- Zhang YW et al. Presenilin/gamma-secretase-dependent processing of beta-amyloid precursor protein regulates EGF receptor expression. Proc Natl Acad Sci U S A 104:10613-8 (2007). ChIP ; Mouse . PubMed: 17556541