Anti-FBXL3 抗体 (ab96645)
Key features and details
- Rabbit polyclonal to FBXL3
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-FBXL3 antibody
FBXL3 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to FBXL3 -
由来種
Rabbit -
アプリケーション
適用あり: WB, ICC/IFmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat, Cow, Zebrafish -
免疫原
Recombinant protein fragment corresponding to a region within amino acids 14 and 279 of Human FBXL3 (NP_036290).
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ポジティブ・コントロール
- 293T, H1299, HeLa, HepG2 and Raji cell lines. MOLT4 whole cell lysate and A431 cells
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab96645の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB | (1) |
1/500 - 1/3000. Predicted molecular weight: 49 kDa.
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ICC/IF |
1/100 - 1/200.
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特記事項 |
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WB
1/500 - 1/3000. Predicted molecular weight: 49 kDa. |
ICC/IF
1/100 - 1/200. |
ターゲット情報
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機能
Substrate-recognition component of some SCF (SKP1-CUL1-F-box protein)-type E3 ubiquitin ligase complex involved in circadian clock function. The SCF(FBXL3) complex acts by mediating ubiquitination and subsequent degradation of CRY1 and CRY2. Recruiter of target protein that may recognize and bind to some phosphorylated proteins and promotes their ubiquitination and degradation. -
組織特異性
Widely expressed. -
配列類似性
Contains 1 F-box domain.
Contains 7 LRR (leucine-rich) repeats. -
細胞内局在
Nucleus. Cytoplasm. Predominantly nuclear. - Information by UniProt
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参照データベース
- Entrez Gene: 26224 Human
- Entrez Gene: 50789 Mouse
- Entrez Gene: 306129 Rat
- Omim: 605653 Human
- SwissProt: Q9UKT7 Human
- SwissProt: Q8C4V4 Mouse
- Unigene: 508284 Human
- Unigene: 214746 Mouse
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別名
- F box and leucine rich repeat protein 3 antibody
- F box and leucine rich repeat protein 3A antibody
- F box protein Fbl3a antibody
see all
画像
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (3)
ab96645 は 3 報の論文で使用されています。
- Ashraf NS et al. Druggable genome screen identifies new regulators of the abundance and toxicity of ATXN3, the Spinocerebellar Ataxia type 3 disease protein. Neurobiol Dis 137:104697 (2020). PubMed: 31783119
- Toledo M et al. Autophagy Regulates the Liver Clock and Glucose Metabolism by Degrading CRY1. Cell Metab 28:268-281.e4 (2018). PubMed: 29937374
- Wang D et al. FBXL3 is regulated by miRNA-4735-3p and suppresses cell proliferation and migration in non-small cell lung cancer. Pathol Res Pract N/A:N/A (2018). PubMed: 30594330