Anti-Fbx32 抗体 (ab74023)
Key features and details
- Rabbit polyclonal to Fbx32
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-Fbx32 antibody
Fbx32 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Fbx32 -
由来種
Rabbit -
特異性
This antibody is only batch tested in WB and therefore we can only guarantee it for this application. Customers have successfully used this antibody in IHC and ICC but we have not tested it's performance in these applications. If you would like an antibody that is tested in ICC, we would recommend our recombinant antibody ab168372. -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Mouse, Rat, Human
交差が予測される動物種: Chicken, Cow, Pig, Zebrafish -
免疫原
Synthetic peptide within Human Fbx32 aa 300 to the C-terminus (internal sequence) conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
(Peptide available asab74022) -
ポジティブ・コントロール
- WB: Human, mouse and rat skeletal muscle tissue lysate.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab74023の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB | (3) |
Use a concentration of 2 µg/ml. Detects a band of approximately 42 kDa (predicted molecular weight: 42 kDa).
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特記事項 |
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WB
Use a concentration of 2 µg/ml. Detects a band of approximately 42 kDa (predicted molecular weight: 42 kDa). |
ターゲット情報
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機能
Substrate recognition component of a (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Probably recognizes and binds to phosphorylated target proteins during skeletal muscle atrophy. Recognizes TERF1. -
組織特異性
Specifically expressed in cardiac and skeletal muscle. -
パスウェイ
Protein modification; protein ubiquitination. -
配列類似性
Contains 1 F-box domain. - Information by UniProt
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参照データベース
- Entrez Gene: 513776 Cow
- Entrez Gene: 114907 Human
- Entrez Gene: 67731 Mouse
- Entrez Gene: 733657 Pig
- Entrez Gene: 171043 Rat
- Omim: 606604 Human
- SwissProt: Q2KHT6 Cow
- SwissProt: Q969P5 Human
see all -
別名
- 4833442G10Rik antibody
- AI430017 antibody
- Atrogin 1 antibody
see all
画像
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All lanes : Anti-Fbx32 antibody (ab74023) at 2 µg/ml
Lane 1 : Human skeletal muscle tissue lysate - total protein (ab29330)
Lane 2 : Mouse skeletal muscle tissue lysate
Lane 3 : Rat skeletal muscle tissue lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 42 kDa
Observed band size: 42 kDa
Additional bands at: 90 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 15 minutes
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (29)
ab74023 は 29 報の論文で使用されています。
- Testa MTJ et al. Resistance Training Attenuates Activation of STAT3 and Muscle Atrophy in Tumor-Bearing Mice. Front Oncol 12:880787 (2022). PubMed: 35847939
- Khin PP et al. Dulaglutide improves muscle function by attenuating inflammation through OPA-1-TLR-9 signaling in aged mice. Aging (Albany NY) 13:21962-21974 (2021). PubMed: 34537761
- Müller E et al. E3-ligase knock down revealed differential titin degradation by autopagy and the ubiquitin proteasome system. Sci Rep 11:21134 (2021). PubMed: 34702928
- Su J et al. The rat model of COPD skeletal muscle dysfunction induced by progressive cigarette smoke exposure: a pilot study. BMC Pulm Med 20:74 (2020). PubMed: 32293377
- Zhou L et al. Herbal complex 'Buyang Huanwu Tang' improves motor endplate function of denervated-dependent skeletal muscle atrophy in rat. J Integr Neurosci 19:89-99 (2020). PubMed: 32259889