Anti-FBP1 抗体 [EPR4619] (ab109020)

Product price, shipping and contact information

Currently unavailable

申し訳ございませんが、現在表示できません。

こちらにお問い合わせいただくか、またはしばらく経ってから改めてお試しください

Loading size & price…

Abpromise

保証された製品品質、優れたカスタマー・サポート。詳細はこちら。

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FBP1 antibody [EPR4619] (ab109020)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR4619] to FBP1
Suitable for: WB, IHC-P
Reacts with: Mouse, Rat, Human

Carrier Free

製品名

Anti-FBP1 antibody [EPR4619]
FBP1 一次抗体製品一覧
製品の詳細

Rabbit monoclonal [EPR4619] to FBP1
由来種

Rabbit
アプリケーション

適用あり: WB, IHC-Pmore details
適用なし: ICC/IF
種交差性

交差種: Mouse, Rat, Human
免疫原

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
ポジティブ･コントロール
- HL60 lysate treated with vitamin D3, MCF7 and Human fetal liver lysates; Human kidney and liver tissues.
特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

製品の状態

Liquid
保存方法

Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
バッファー

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA, 59% PBS
Concentration information loading...
精製度

Protein A purified
ポリ/モノ

モノクローナル
クローン名

EPR4619
アイソタイプ

IgG
研究分野
- Neuroscience
- Development

Alternative Versions
- Anti-FBP1 antibody [EPR4619] - BSA and Azide free (ab247749)
Isotype control
- Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
Recombinant Protein
- Recombinant human FBP1 protein (Active) (ab208306)

The Abpromise guarantee

Abpromise保証は、次のテスト済みアプリケーションにおけるab109020の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション	Abreviews	特記事項
WB		1/1000 - 1/10000. Predicted molecular weight: 37 kDa.
IHC-P		1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Heat up to 98 &degC, below boiling, and then let cool for 10-20 min.

特記事項
WB 1/1000 - 1/10000. Predicted molecular weight: 37 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Heat up to 98 &degC, below boiling, and then let cool for 10-20 min.

追加情報

Is unsuitable for ICC/IF.

パスウェイ

Carbohydrate biosynthesis; gluconeogenesis.
関連疾患

Defects in FBP1 are the cause of fructose-1,6-bisphosphatase deficiency (FBPD) [MIM:229700]. FBPD is inherited as an autosomal recessive disorder mainly in the liver and causes life-threatening episodes of hypoglycemia and metabolic acidosis (lactacidemia) in newborn infants or young children.
配列類似性

Belongs to the FBPase class 1 family.
Target information above from: UniProt accession P09467 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
参照データベース
- Entrez Gene: 2203 Human
- Entrez Gene: 14121 Mouse
- Entrez Gene: 24362 Rat
- Omim: 611570 Human
- SwissProt: P09467 Human
- SwissProt: Q9QXD6 Mouse
- SwissProt: P19112 Rat
- Unigene: 494496 Human
- Unigene: 423078 Mouse
- Unigene: 33703 Rat
see all
別名
- 6-bisphosphatase 1 antibody
- 6-bisphosphate 1-phosphohydrolase 1 antibody
- D fructose 1 6 bisphosphate 1 phosphohydrolase 1 antibody
- D-fructose-1 antibody
- EC 3.1.3.11 antibody
- F16P1_HUMAN antibody
- FBP antibody
- FBP 1 antibody
- FBP1 antibody
- FBPase 1 antibody
- Fructose 1 6 bisphosphatase 1 antibody
- Fructose bisphosphatase 1 antibody
- Fructose-1 antibody
- Growth inhibiting protein 17 antibody
- Liver fructose bisphosphatase antibody
see all

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FBP1 antibody [EPR4619] (ab109020)

ab109020 at 1:500 staining Anti-FBP1 antibody in mouse kidney tissue by immunohistochemistry (FFPE). Immunohistochemical analysis of paraffin-embedded mouse kidney tissue labeling FBP1 with ab109020 at 1/500 dilution followed by Goat Anti-Rabbit IgG H&L (HRP). Counter stained with hematoxylin.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Western blot - Anti-FBP1 antibody [EPR4619] (ab109020)

All lanes : Anti-FBP1 antibody [EPR4619] (ab109020) at 1/10000 dilution (purified)

Lane 1 : Mouse liver
Lane 2 : Rat liver

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : HRP goat anti-rabbit (H+L) at 1/1000 dilution

Predicted band size: 37 kDa
Observed band size: 37 kDa

Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
Western blot - Anti-FBP1 antibody [EPR4619] (ab109020)

All lanes : Anti-FBP1 antibody [EPR4619] (ab109020) at 1/2000 dilution (purified)

Lane 1 : MCF7 cell lysate
Lane 2 : HL-60 cell lysate treated with vitamin D3

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : HRP goat anti-rabbit (H+L) at 1/2000 dilution

Predicted band size: 37 kDa
Observed band size: 37 kDa

Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FBP1 antibody [EPR4619] (ab109020)

ab109020 at 1:500 staining Anti-FBP1 antibody in human liver tissue by immunohistochemistry (FFPE). Immunohistochemical analysis of paraffin-embedded human liver tissue labeling FBP1 with ab109020 at 1/500 dilution followed by Goat Anti-Rabbit IgG H&L (HRP). Counter stained with hematoxylin.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Western blot - Anti-FBP1 antibody [EPR4619] (ab109020)

All lanes : Anti-FBP1 antibody [EPR4619] (ab109020) at 1/1000 dilution (unpurified)

Lane 1 : MCF7 lysate
Lane 2 : Human fetal liver lysate
Lane 3 : HL60 lysate, treated with vitamin D3

Lysates/proteins at 10 µg per lane.

Predicted band size: 37 kDa

Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FBP1 antibody [EPR4619] (ab109020)

Unpurified ab109020 at 1/50 dilution staining FBP1 in Human Kidney by immunohistochemistry, paraffin-embedded tissue.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FBP1 antibody [EPR4619] (ab109020)

Unpurified ab109020 at 1/50 dilution staining FBP1 in Human liver by immunohistochemistry, paraffin-embedded tissue.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Anti-FBP1 antibody [EPR4619] (ab109020)

Western blot protocols
Immunohistochemistry protocols

Click here to view the general protocols

Datasheet download

Download

ab109020 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab109020 は 12 報の論文で使用されています。

Sadiku P et al. Neutrophils Fuel Effective Immune Responses through Gluconeogenesis and Glycogenesis. Cell Metab 33:411-423.e4 (2021). PubMed: 33306983
Fan Z et al. LOXL2 upregulates hypoxia-inducible factor-1a signaling through Snail-FBP1 axis in hepatocellular carcinoma cells. Oncol Rep 43:1641-1649 (2020). PubMed: 32323822
Becker LM et al. Epigenetic Reprogramming of Cancer-Associated Fibroblasts Deregulates Glucose Metabolism and Facilitates Progression of Breast Cancer. Cell Rep 31:107701 (2020). PubMed: 32492417
Aljaylani A et al. Acid Loading Unmasks Glucose Homeostatic Instability in Proximal-Tubule-Targeted Insulin/Insulin-Like-Growth-Factor-1 Receptor Dual Knockout Mice. Cell Physiol Biochem 54:682-695 (2020). PubMed: 32678535
Selmi-Ruby S et al. In Vivo Characterization of the Toxicological Properties of DPhP, One of the Main Degradation Products of Aryl Phosphate Esters. Environ Health Perspect 128:127006 (2020). PubMed: 33296241

View all Publications for this product

レビューと Q&A

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Switch on our new BETA site

Now available

In the coming months

Key features and details

Related conjugates and formulations

製品の概要

製品名

製品の詳細

由来種

アプリケーション

種交差性

免疫原

ポジティブ･コントロール

特記事項

製品の特性

製品の状態

保存方法

バッファー

精製度

ポリ/モノ

クローン名

アイソタイプ

研究分野

関連製品

Alternative Versions

Isotype control

Recombinant Protein

アプリケーション

The Abpromise guarantee

ターゲット情報

パスウェイ

関連疾患

配列類似性

参照データベース

別名

画像

プロトコール

データシートおよび資料

Datasheet download

参考文献 (12)

レビューと Q&A