Anti-Fatty Acid Synthase 抗体 [EPR7465] (ab128856)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR7465] to Fatty Acid Synthase
- Suitable for: WB, Flow Cyt, ICC/IF
- Knockout validated
- Reacts with: Mouse, Rat, Human
製品の概要
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製品名
Anti-Fatty Acid Synthase antibody [EPR7465]
Fatty Acid Synthase 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR7465] to Fatty Acid Synthase -
由来種
Rabbit -
アプリケーション
適用あり: WB, Flow Cyt, ICC/IFmore details
適用なし: IHC-P or IP -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Synthetic peptide within Human Fatty Acid Synthase aa 1-100. The exact sequence is proprietary.
Database link: P49327 -
ポジティブ･コントロール
- WB: HeLa, 293T, A549, SHSY-5Y and MOLT4 cell lysates. ICC/IF: A549 cells. IHC-Fr: Human peritoneal tumor tissue. Flow Cytometry: A549 cells.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
解離定数（KD 値）
KD = 1.58 x 10 -11 M Learn more about KD -
バッファー
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 0.31% Sodium citrate, 0.175% Sodium chloride, 0.0172% EDTA disodium salt, 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR7465 -
アイソタイプ
IgG -
研究分野
- Metabolism
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Lipid metabolism
- Metabolism
- Pathways and Processes
- Metabolic signaling pathways
- Energy transfer pathways
- Energy Metabolism
関連製品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
Our Abpromise guarantee covers the use of ab128856 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | 1/1000 - 1/10000. Predicted molecular weight: 273 kDa. | |
| Flow Cyt | 1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
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| ICC/IF | 1/250 - 1/500. |
ターゲット情報
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機能
Fatty acid synthetase catalyzes the formation of long-chain fatty acids from acetyl-CoA, malonyl-CoA and NADPH. This multifunctional protein has 7 catalytic activities and an acyl carrier protein. -
組織特異性
Ubiquitous. Prominent expression in brain, lung, and liver. -
配列類似性
Contains 1 acyl carrier domain. -
細胞内局在
Cytoplasm. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. - Information by UniProt
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参照データベース
- Entrez Gene: 2194 Human
- Entrez Gene: 14104 Mouse
- Entrez Gene: 50671 Rat
- Omim: 600212 Human
- SwissProt: P49327 Human
- SwissProt: P19096 Mouse
- SwissProt: P12785 Rat
- Unigene: 83190 Human
see all -
別名
- [Acyl-carrier-protein] S acetyltransferase antibody
- [Acyl-carrier-protein] S malonyltransferase antibody
- 3-hydroxypalmitoyl-[acyl-carrier-protein] dehydratase antibody
see all
画像
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![Western blot - Anti-Fatty Acid Synthase antibody [EPR7465] (ab128856)](https://www.abcam.co.jp/ps/products/128/ab128856/Images/ab128856-260969-anti-fatty-acid-synthase-antibody-epr7465-western-blot.jpg)
Western blot - Anti-Fatty Acid Synthase antibody [EPR7465] (ab128856)Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: Fatty Acid Synthase knockout HAP1 cell lysate (20 µg)
Lane 3: A549 cell lysate (20 µg)
Lane 4: Hu liver tissue lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab128856 observed at 250 kDa. Red - loading control, ab18058, observed at 124 kDa.ab128856 was shown to specifically react with Fatty Acid Synthase in wild-type HAP1 cells. No band was observed when Fatty Acid Synthase knockout samples were examined. Wild-type and Fatty Acid Synthase knockout samples were subjected to SDS-PAGE. ab128856 and ab18058 (loading control to Vinculin) were diluted at 1/1000 and 1/10,000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.
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![Western blot - Anti-Fatty Acid Synthase antibody [EPR7465] (ab128856)](https://www.abcam.co.jp/ps/products/128/ab128856/Images/ab128856-142613-anti-fatty-acid-synthase-antibody-epr7465-western-blot.jpg)
Western blot - Anti-Fatty Acid Synthase antibody [EPR7465] (ab128856)All lanes : Anti-Fatty Acid Synthase antibody [EPR7465] (ab128856) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : 293T cell lysate
Lane 3 : A549 cell lysate
Lane 4 : SHSY-5Y cell lysate
Lane 5 : MOLT4 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 273 kDa -
![Immunocytochemistry/ Immunofluorescence - Anti-Fatty Acid Synthase antibody [EPR7465] (ab128856)](https://www.abcam.co.jp/ps/products/128/ab128856/Images/ab128856-142612-anti-fatty-acid-synthase-antibody-epr7465-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Fatty Acid Synthase antibody [EPR7465] (ab128856)ab128856 at a 1/250 dilution staining Fatty Acid Synthase in A549 cells by immunofluorescence. -
![Flow Cytometry - Anti-Fatty Acid Synthase antibody [EPR7465] (ab128856)](https://www.abcam.co.jp/ps/products/128/ab128856/Images/ab128856-238332-anti-fatty-acid-synthase-antibody-epr7465-flow-cytometry.jpg)
Flow Cytometry - Anti-Fatty Acid Synthase antibody [EPR7465] (ab128856)Overlay histogram showing A549 cells stained with ab128856 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab128856, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in A549 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions. -
![OI-RD Scanning - Anti-Fatty Acid Synthase antibody [EPR7465] (ab128856)](https://www.abcam.co.jp/ps/products/128/ab128856/Images/ab128856-142611-anti-fatty-acid-synthase-antibody-epr7465.jpg)
OI-RD Scanning - Anti-Fatty Acid Synthase antibody [EPR7465] (ab128856) -
![Anti-Fatty Acid Synthase antibody [EPR7465] (ab128856)](https://www.abcam.co.jp/ps/products/128/ab128856/Images/ab128856-7-benefits-of-recombinant-antibodies.png)
Anti-Fatty Acid Synthase antibody [EPR7465] (ab128856)
プロトコール
参考文献 (11)
ab128856 は 11 報の論文で使用されています。
- Ma X et al. Mechanism of continuous high temperature affecting growth performance, meat quality, and muscle biochemical properties of finishing pigs. Genes Nutr 14:23 (2019). PubMed: 31367261
- Xue D et al. Quantitative proteomic analysis of sperm in unexplained recurrent pregnancy loss. Reprod Biol Endocrinol 17:52 (2019). PubMed: 31288842
- Wu Y et al. Berberine (BBR) Attenuated Palmitic Acid (PA)-Induced Lipotoxicity in Human HK-2 Cells by Promoting Peroxisome Proliferator-Activated Receptor a (PPAR-a). Med Sci Monit 25:7702-7708 (2019). PubMed: 31607744
- Tao XR et al. Angiotensinogen in hepatocytes contributes to Western diet-induced liver steatosis. J Lipid Res 60:1983-1995 (2019). PubMed: 31604805
- Carbonetti G et al. FABP5 coordinates lipid signaling that promotes prostate cancer metastasis. Sci Rep 9:18944 (2019). PubMed: 31831821
