Anti-Fatty Acid Synthase 抗体 [EPR7465] (ab128856)
![Western blot - Anti-Fatty Acid Synthase antibody [EPR7465] (ab128856)](https://www.abcam.co.jp/ps/products/128/ab128856/Images/ab128856-482474-anti-fatty-acid-synthase-antibody-epr7465-westernblot-human-mouse-rat.jpg "Western blot - Anti-Fatty Acid Synthase antibody [EPR7465] (ab128856)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR7465] to Fatty Acid Synthase
- Suitable for: WB, ICC/IF, Flow Cyt (Intra)
- Knockout validated
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
製品の概要
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製品名
Anti-Fatty Acid Synthase antibody [EPR7465]
Fatty Acid Synthase 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR7465] to Fatty Acid Synthase -
由来種
Rabbit -
アプリケーション
適用あり: WB, ICC/IF, Flow Cyt (Intra)more details
適用なし: IHC-P or IP -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Synthetic peptide within Human Fatty Acid Synthase aa 1-100. The exact sequence is proprietary.
Database link: P49327 -
ポジティブ･コントロール
- WB: HeLa, HEK-293, 293T, A549, SHSY-5Y, NIH/3T3, L6 (Rat skeletal muscle myoblast) and MOLT4 cell lysates; Mouse brain, Rat brain lysates. ICC/IF: A549 cells. IHC-Fr: Human peritoneal tumor tissue. Flow Cyt (intra): A549 cells.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
解離定数（KD 値）
KD = 1.58 x 10 -11 M Learn more about KD -
バッファー
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR7465 -
アイソタイプ
IgG -
研究分野
- Metabolism
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Lipid metabolism
- Metabolism
- Pathways and Processes
- Metabolic signaling pathways
- Energy transfer pathways
- Energy Metabolism
関連製品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab128856の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | (2) |
1/1000 - 1/10000. Predicted molecular weight: 273 kDa.
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| ICC/IF | (1) |
1/50.
For unpurified version, use at 1/250 - 1/500 dilution. |
| Flow Cyt (Intra) |
1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
| 特記事項 |
|---|
|
WB
1/1000 - 1/10000. Predicted molecular weight: 273 kDa. |
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ICC/IF
1/50. For unpurified version, use at 1/250 - 1/500 dilution. |
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Flow Cyt (Intra)
1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
ターゲット情報
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機能
Fatty acid synthetase catalyzes the formation of long-chain fatty acids from acetyl-CoA, malonyl-CoA and NADPH. This multifunctional protein has 7 catalytic activities and an acyl carrier protein. -
組織特異性
Ubiquitous. Prominent expression in brain, lung, and liver. -
配列類似性
Contains 1 acyl carrier domain. -
細胞内局在
Cytoplasm. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. - Information by UniProt
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参照データベース
- Entrez Gene: 2194 Human
- Entrez Gene: 14104 Mouse
- Entrez Gene: 50671 Rat
- Omim: 600212 Human
- SwissProt: P49327 Human
- SwissProt: P19096 Mouse
- SwissProt: P12785 Rat
- Unigene: 83190 Human
see all -
別名
- [Acyl-carrier-protein] S acetyltransferase antibody
- [Acyl-carrier-protein] S malonyltransferase antibody
- 3-hydroxypalmitoyl-[acyl-carrier-protein] dehydratase antibody
see all
画像
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Western blot - Anti-Fatty Acid Synthase antibody [EPR7465] (ab128856)All lanes : Anti-Fatty Acid Synthase antibody [EPR7465] (ab128856) at 1/5000 dilution (Purified)
Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate
Lane 2 : HEK-293 (Human embryonic kidney epithelial cell) whole cell lysate
Lane 3 : A549 (Human lung carcinoma epithelial cell) whole cell lysate
Lane 4 : MOLT-4 (Human lymphoblastic leukemia T lymphoblast) whole cell lysate
Lane 5 : SH-SY5Y (Human neuroblastoma epithelial cell) whole cell lysate
Lane 6 : Mouse brain lysate
Lane 7 : NIH/3T3 (Mouse embryonic fibroblast) whole cell lysate
Lane 8 : Rat brain lysate
Lane 9 : L6 (Rat skeletal muscle myoblast) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 273 kDa
Observed band size: 273 kDa -
![Flow Cytometry - Anti-Fatty Acid Synthase antibody [EPR7465] (ab128856)](https://www.abcam.co.jp/ps/products/128/ab128856/Images/ab128856-10-anti-fatty-acid-synthase-antibody-epr7465-flowcytometry-a549-human.jpg)
Flow Cytometry - Anti-Fatty Acid Synthase antibody [EPR7465] (ab128856)Flow Cytometry analysis of A549 (Human lung carcinoma epithelial cell) cells labelling Fatty Acid Synthase with Purified ab128856 at 1:50 dilution (10 µg/ml) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1:2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabelled control - Cell without incubation with primary antibody and secondary antibody (Blue). -
![Immunocytochemistry - Anti-Fatty Acid Synthase antibody [EPR7465] (ab128856)](https://www.abcam.co.jp/ps/products/128/ab128856/Images/ab128856-9-anti-fatty-acid-synthase-antibody-epr7465-immunocytochemistry-hela-human.jpg)
Immunocytochemistry - Anti-Fatty Acid Synthase antibody [EPR7465] (ab128856)Immunocytochemistry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling Fatty Acid Synthase with Purified ab128856 at 1:50 dilution (4.5 µg/ml). Cells were fixed in 100% Methanol and permeabilized with 0.1% tritonX-100. Cells were counterstained with . Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control. -
![Western blot - Anti-Fatty Acid Synthase antibody [EPR7465] (ab128856)](https://www.abcam.co.jp/ps/products/128/ab128856/Images/ab128856-260969-anti-fatty-acid-synthase-antibody-epr7465-western-blot.jpg)
Western blot - Anti-Fatty Acid Synthase antibody [EPR7465] (ab128856)Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: Fatty Acid Synthase knockout HAP1 cell lysate (20 µg)
Lane 3: A549 cell lysate (20 µg)
Lane 4: Hu liver tissue lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab128856 observed at 250 kDa. Red - loading control, ab18058, observed at 124 kDa.ab128856 was shown to specifically react with Fatty Acid Synthase in wild-type HAP1 cells. No band was observed when Fatty Acid Synthase knockout samples were examined. Wild-type and Fatty Acid Synthase knockout samples were subjected to SDS-PAGE. ab128856 and ab18058 (loading control to Vinculin) were diluted at 1/1000 and 1/10,000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.
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![Anti-Fatty Acid Synthase antibody [EPR7465] (ab128856)](https://www.abcam.co.jp/ps/products/128/ab128856/Images/ab128856-7-benefits-of-recombinant-antibodies.png)
Anti-Fatty Acid Synthase antibody [EPR7465] (ab128856)
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (12)
ab128856 は 12 報の論文で使用されています。
- Zhang H et al. Vitamin D receptor targets hepatocyte nuclear factor 4a and mediates protective effects of vitamin D in nonalcoholic fatty liver disease. J Biol Chem 295:3891-3905 (2020). PubMed: 32051143
- Ma X et al. Mechanism of continuous high temperature affecting growth performance, meat quality, and muscle biochemical properties of finishing pigs. Genes Nutr 14:23 (2019). PubMed: 31367261
- Xue D et al. Quantitative proteomic analysis of sperm in unexplained recurrent pregnancy loss. Reprod Biol Endocrinol 17:52 (2019). PubMed: 31288842
- Wu Y et al. Berberine (BBR) Attenuated Palmitic Acid (PA)-Induced Lipotoxicity in Human HK-2 Cells by Promoting Peroxisome Proliferator-Activated Receptor a (PPAR-a). Med Sci Monit 25:7702-7708 (2019). PubMed: 31607744
- Tao XR et al. Angiotensinogen in hepatocytes contributes to Western diet-induced liver steatosis. J Lipid Res 60:1983-1995 (2019). PubMed: 31604805