Application
Western blot
Sample
Rat Tissue lysate - whole (Rat liver tissue)
Loading amount
140 µg
Specification
Rat liver tissue
Gel Running Conditions
Reduced Denaturing (6% gel. Sample was heated in laemli buffer at 70 C for 10 min before loading)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Dilution
1/1000
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% BSA in TBS-T(0.1%)
Secondary antibody
Name
Non-Abcam antibody was used: Anti-rabbit IgG, HRP-linked Antibody
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/1000
Detection
Detection method
Pierce ECL West Pico
Exposure
2 minute(s) and 0 second(s)
Bands
Specific: 273 kDa
Additional data
Additional Notes
lane1: control rat
lane 2: control rats given plant extract
lane 2: control rats given plant extract
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Mrs. Smitha Koshy
Verified customer
投稿 Aug 13 2012