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    fatty-acid-synthase-antibody-ab22759.pdf

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遺伝子ノックアウト細胞株 検証済

Anti-Fatty Acid Synthase 抗体 (ab22759)

  • Datasheet
  • SDS
Reviews (14)Q&A (2)References (88)

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Immunocytochemistry - Anti-Fatty Acid Synthase antibody (ab22759)
  • Western blot - Anti-Fatty Acid Synthase antibody (ab22759)
  • Western blot - Anti-Fatty Acid Synthase antibody (ab22759)
  • Immunohistochemistry (PFA fixed) - Anti-Fatty Acid Synthase antibody (ab22759)

Key features and details

  • Rabbit polyclonal to Fatty Acid Synthase
  • Suitable for: WB, IHC (PFA fixed), ICC
  • Knockout validated
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG

リコンビナント抗体で、ロット間での高い再現性を実現

Product image
Anti-Fatty Acid Synthase antibody [EPR7466] (ab128870)
  • 異なるロット間での安定した再現性
  • 容易なスケールアップ
  • 評価試験による特異性の確認済み
  • 倫理基準に準拠 - アニマル・フリーの生産

製品の概要

  • 製品名

    Anti-Fatty Acid Synthase antibody
    Fatty Acid Synthase 一次抗体 製品一覧
  • 製品の詳細

    Rabbit polyclonal to Fatty Acid Synthase
  • 由来種

    Rabbit
  • アプリケーション

    適用あり: WB, IHC (PFA fixed), ICCmore details
  • 種交差性

    交差種: Mouse, Rat, Human
    交差が予測される動物種: Chicken, Cow
  • 免疫原

    Synthetic peptide corresponding to Mouse Fatty Acid Synthase aa 2450 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab25719)

  • 特記事項

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

  • 製品の状態

    Liquid
  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファー

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS

    Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • 精製度

    Immunogen affinity purified
  • ポリ/モノ

    ポリクローナル
  • アイソタイプ

    IgG
  • 研究分野

    • Cardiovascular
    • Lipids / Lipoproteins
    • Fatty Acids
    • Synthesis
    • Signal Transduction
    • Metabolism
    • Energy Metabolism
    • Neuroscience
    • Neurology process
    • Metabolism
    • Signal Transduction
    • Metabolism
    • Lipid metabolism
    • Cancer
    • Tumor biomarkers
    • Enzymes
    • Other
    • Cancer
    • Cancer Metabolism
    • Metabolic signaling pathway
    • Metabolism of lipids and lipoproteins
    • Cancer
    • Cancer Metabolism
    • Metabolic signaling pathway
    • Integration of energy metabolism
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Lipid and lipoprotein metabolism
    • Lipid metabolism
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    • Pathways and Processes
    • Metabolic signaling pathways
    • Energy transfer pathways
    • Energy Metabolism
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
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    • Integration of energy
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    • Types of disease
    • Obesity
    • Metabolism
    • Types of disease
    • Cancer
    • Metabolism
    • Pathways and Processes
    • Redox metabolism
    • Fatty acid oxidation

関連製品

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant Human Fatty Acid Synthase protein (ab131929)

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab22759の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB (7)
Use at an assay dependent concentration. Detects a band of approximately 273 kDa (predicted molecular weight: 273 kDa).
IHC (PFA fixed)
Use a concentration of 2 µg/ml.
ICC
Use a concentration of 5 µg/ml.
特記事項
WB
Use at an assay dependent concentration. Detects a band of approximately 273 kDa (predicted molecular weight: 273 kDa).
IHC (PFA fixed)
Use a concentration of 2 µg/ml.
ICC
Use a concentration of 5 µg/ml.

ターゲット情報

  • 機能

    Fatty acid synthetase catalyzes the formation of long-chain fatty acids from acetyl-CoA, malonyl-CoA and NADPH. This multifunctional protein has 7 catalytic activities and an acyl carrier protein.
  • 組織特異性

    Ubiquitous. Prominent expression in brain, lung, and liver.
  • 配列類似性

    Contains 1 acyl carrier domain.
  • 細胞内局在

    Cytoplasm. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Target information above from: UniProt accession P49327 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 参照データベース

    • Entrez Gene: 281152 Cow
    • Entrez Gene: 2194 Human
    • Entrez Gene: 14104 Mouse
    • Entrez Gene: 50671 Rat
    • Omim: 600212 Human
    • SwissProt: P12276 Chicken
    • SwissProt: Q71SP7 Cow
    • SwissProt: P49327 Human
    • SwissProt: P19096 Mouse
    • SwissProt: P12785 Rat
    • Unigene: 83190 Human
    • Unigene: 236443 Mouse
    • Unigene: 9486 Rat
    see all
  • 別名

    • [Acyl-carrier-protein] S acetyltransferase antibody
    • [Acyl-carrier-protein] S malonyltransferase antibody
    • 3-hydroxypalmitoyl-[acyl-carrier-protein] dehydratase antibody
    • 3-oxoacyl-[acyl-carrier-protein] reductase antibody
    • 3-oxoacyl-[acyl-carrier-protein] synthase antibody
    • Enoyl-[acyl-carrier-protein] reductase antibody
    • FAS antibody
    • FAS_HUMAN antibody
    • FASN antibody
    • Fatty acid synthase antibody
    • MGC14367 antibody
    • MGC15706 antibody
    • OA 519 antibody
    • Oleoyl-[acyl-carrier-protein] hydrolase antibody
    • SDR27X1 antibody
    • Short chain dehydrogenase/reductase family 27X member 1 antibody
    see all

画像

  • Immunocytochemistry - Anti-Fatty Acid Synthase antibody (ab22759)
    Immunocytochemistry - Anti-Fatty Acid Synthase antibody (ab22759)

    ab22759 staining Fatty Acid Synthase in HeLa cells. The cells were fixed with 4% paraformaldehyde (10 min), permeabilized with 0.1% PBS-Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab22759 at 5 µg/ml and ab7291, Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control. Cells were then incubated with ab150081, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 488), pre-adsorbed at 1/1000 dilution (shown in green) and ab150120, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 594), pre-adsorbed at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).

    Also suitable in cells fixed with 100% methanol (5 min).

    Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.

  • Western blot - Anti-Fatty Acid Synthase antibody (ab22759)
    Western blot - Anti-Fatty Acid Synthase antibody (ab22759)

    Lane 1: Wild-type HAP1 cell lysate (20 µg)
    Lane 2: Fatty Acid Synthase knockout HAP1 cell lysate (20 µg)
    Lane 3: A549 cell lysate (20 µg)
    Lane 4: Hu liver tissue lysate (20 µg)
    Lanes 1 - 4: Merged signal (red and green). Green - ab22759 observed at 250 kDa. Red - loading control, ab18058, observed at 124 kDa.

    ab22759 was shown to specifically react with Fatty Acid Synthase in wild-type HAP1 cells. No band was observed when Fatty Acid Synthase knockout samples were examined. Wild-type and Fatty Acid Synthase knockout samples were subjected to SDS-PAGE. ab22759 and ab18058 (loading control to Vinculin) were diluted at 1 µg/ml and 1/10,000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.

  • Western blot - Anti-Fatty Acid Synthase antibody (ab22759)
    Western blot - Anti-Fatty Acid Synthase antibody (ab22759)
    All lanes : Anti-Fatty Acid Synthase antibody (ab22759) at 1 µg/ml

    Lane 1 : 3T3-L1 nuclear extract lysate (ab14632)
    Lane 2 : Brain (Mouse) Tissue Lysate (ab27253)
    Lane 3 : Liver (Mouse) Tissue Lysate (ab7935)
    Lane 4 : 3T3-L1 nuclear extract lysate (ab14632) with Mouse Fatty Acid Synthase peptide (ab25719) at 1 µg/ml
    Lane 5 : Brain (Mouse) Tissue Lysate (ab27253) with Mouse Fatty Acid Synthase peptide (ab25719) at 1 µg/ml
    Lane 6 : Liver (Mouse) Tissue Lysate (ab7935) with Mouse Fatty Acid Synthase peptide (ab25719) at 1 µg/ml

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Alexa Fluor Goat polyclonal to Rabbit IgG (700) at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size: 273 kDa
    Observed band size: 273 kDa
    Additional bands at: 100 kDa, 35 kDa, 50 kDa (possible IgG). We are unsure as to the identity of these extra bands.

  • Immunohistochemistry (PFA fixed) - Anti-Fatty Acid Synthase antibody (ab22759)
    Immunohistochemistry (PFA fixed) - Anti-Fatty Acid Synthase antibody (ab22759)This image is courtesy of Sophie Pezet, King's College London, United Kingdom

    Immunofluorescent staining for Fatty Acid Synthase in the rat striatum using Rabbit polyclonal to Fatty Acid Synthase (ab22759). Abundant staining was observed in the Striatum with lower levels of staining observed in the Corpus callosum. This is a montage of three pictures aquired using a X10 objective. ab22759 was used at 1/200 (2µg/ml) incubated overnight at room temperature.

    Secondary antibody used was anti-rabbit Alexa Fluor® 488 at 1/1000 incubated for 2 hours at room temperature. Rat brain tissue was perfusion fixed with 4% PFA followed by overnight post-fixation in the same fixative, cryoprotected in 20% sucrose and frozen in OCT. 30µm coronal sections were cut on a cyrostat and immunohistochemistry performed by the 'free floating' technique.

プロトコール

  • Immunohistochemistry protocols
  • Immunocytochemistry & immunofluorescence protocols
  • Western blot protocols

Click here to view the general protocols

データシートおよび資料

  • SDS download

  • Datasheet download

    Download

参考文献 (88)

ab22759 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab22759 は 88 報の論文で使用されています。

  • Hulse M  et al. Epstein-Barr Virus-Encoded Latent Membrane Protein 1 and B-Cell Growth Transformation Induce Lipogenesis through Fatty Acid Synthase. J Virol 95:N/A (2021). PubMed: 33208446
  • Sheard JJ  et al. Combined bezafibrate, medroxyprogesterone acetate and valproic acid treatment inhibits osteosarcoma cell growth without adversely affecting normal mesenchymal stem cells. Biosci Rep 41:N/A (2021). PubMed: 33289496
  • Schmidt NH  et al. High-fructose feeding does not induce steatosis or non-alcoholic fatty liver disease in pigs. Sci Rep 11:2807 (2021). PubMed: 33531575
  • Zhong X  et al. Upregulation of fatty acid synthase in MYC and BCL-2 double-expressor lymphoma. Oncol Lett 21:245 (2021). PubMed: 33664809
  • Wu W  et al. MicroRNA-29b/29c targeting CTRP6 influences porcine adipogenesis via the AKT/PKA/MAPK Signalling pathway. Adipocyte 10:264-274 (2021). PubMed: 33938394
View all Publications for this product

レビューと Q&A

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レビューを送る 質問を送る

1-10 of 16 Abreviews or Q&A

Western blot abreview for Anti-Fatty Acid Synthase antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Mouse Tissue lysate - whole (Liver)
Gel Running Conditions
Reduced Denaturing (8%)
Loading amount
50 µg
Treatment
Western diet 12 weeks
Specification
Liver
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Read More

Abcam user community

Verified customer

投稿 Sep 10 2018

Western blot abreview for Anti-Fatty Acid Synthase antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Rat Tissue lysate - whole (LIVER)
Gel Running Conditions
Reduced Denaturing
Loading amount
50 µg
Specification
LIVER
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More

Abcam user community

Verified customer

投稿 Oct 17 2016

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Fatty Acid Synthase antibody

Good
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 1.5% · Temperature: 23°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citra Plus
Sample
Mouse Tissue sections (Colon)
Specification
Colon
Permeabilization
No
Fixative
Formaldehyde
Read More

Abcam user community

Verified customer

投稿 Aug 28 2013

Western blot abreview for Anti-Fatty Acid Synthase antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (293T, HepG2, MEF)
Loading amount
20 µg
Specification
293T, HepG2, MEF
Gel Running Conditions
Reduced Denaturing (8%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 7% · Temperature: 20°C
Read More

Mr. Dongil Kim

Verified customer

投稿 Jan 15 2013

Western blot abreview for Anti-Fatty Acid Synthase antibody

Good
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Mouse Tissue lysate - whole (Liver)
Loading amount
35 µg
Specification
Liver
Gel Running Conditions
Reduced Denaturing (8% SDS-PAGE)
Blocking step
odyssey blocking buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 37°C
Read More

Mr. Ahmed Nayef

Verified customer

投稿 Dec 26 2012

Immunocytochemistry/ Immunofluorescence abreview for Anti-Fatty Acid Synthase antibody

Good
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (C2C12)
Specification
C2C12
Fixative
Formaldehyde
Permeabilization
Yes - 0.1% Triton X-100
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 25°C
Read More

Abcam user community

Verified customer

投稿 Sep 21 2012

Western blot abreview for Anti-Fatty Acid Synthase antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Rat Tissue lysate - whole (Rat liver tissue)
Loading amount
140 µg
Specification
Rat liver tissue
Gel Running Conditions
Reduced Denaturing (6% gel. Sample was heated in laemli buffer at 70 C for 10 min before loading)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More

Mrs. Smitha Koshy

Verified customer

投稿 Aug 13 2012

Immunoprecipitation abreview for Anti-Fatty Acid Synthase antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Immunoprecipitation
Sample
Mouse Cell lysate - whole cell (C2C12)
Total protein in input
200 µg
Specification
C2C12
Immuno-precipitation step
Protein A
Read More

Abcam user community

Verified customer

投稿 Mar 08 2012

Western blot abreview for Anti-Fatty Acid Synthase antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Mouse Tissue lysate - whole (muscle)
Loading amount
20 µg
Specification
muscle
Gel Running Conditions
Reduced Denaturing
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More

Abcam user community

Verified customer

投稿 Mar 25 2011

Question

Hi Abcam tech support,

I had a customer asking about antibody ab22759 anti Fatty Acid Synthase. They were asking if the antibody has ever been tested in pig samples. I told him it had probably never been tested, but they asked if it would be possible to run the immunogen sequence against the pig version to try determine the degree of homology. Would this be possible?

Best regards,

Read More

Abcam community

Verified customer

Asked on Jun 21 2012

Answer

Thank you for your email.

We have not tested ab22759 with pig samples at this time, but I aligned the immunogen sequence against the pig sequence (UniProt accession linked below, in case the customer is wondering what sequence was used) and found they are 93% identical. There is only one amino acid that is different out of 15 total amino acids. So, it will likely cross-react but we are not sure and can not guarantee this until it is tested.

http://www.uniprot.org/uniprot/A5YV76

We do have a testing discount program that your customer may be interested in. In this program, they would purchase the antibody, test it with pig samples, and in exchange we will give them one free antibody on a future purchase. Please let me know if the customer would like more information about this program.

I hope this will be useful, but if you have further questions or if there is anything else that we can do for you please let me know and I'll be happy to help.

Read More

Abcam Scientific Support

Answered on Jun 21 2012

1-10 of 16 Abreviews or Q&A

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